WO2018194437A3 - 표적 점 돌연변이 유전자 검출을 위한 핵산 분자 템플레이트 및 이를 이용한 유전자 검사방법 - Google Patents

표적 점 돌연변이 유전자 검출을 위한 핵산 분자 템플레이트 및 이를 이용한 유전자 검사방법 Download PDF

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WO2018194437A3
WO2018194437A3 PCT/KR2018/004700 KR2018004700W WO2018194437A3 WO 2018194437 A3 WO2018194437 A3 WO 2018194437A3 KR 2018004700 W KR2018004700 W KR 2018004700W WO 2018194437 A3 WO2018194437 A3 WO 2018194437A3
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point mutation
target point
mutation gene
same
nucleic acid
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PCT/KR2018/004700
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French (fr)
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WO2018194437A2 (ko
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이혁진
김지수
최보람
김주영
조주연
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이화여자대학교 산학협력단
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Publication of WO2018194437A2 publication Critical patent/WO2018194437A2/ko
Publication of WO2018194437A3 publication Critical patent/WO2018194437A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
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  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 표적 점 돌연변이 유전자 검출용 템플레이트, 및 이를 이용한 표적 점 돌연변이 유전자를 검출하는 방법에 관한 것으로, 본 발명에 따른 점 돌연변이 검출용 템플레이트를 이용한 표적 점 돌연변이 유전자의 검출방법은 표적 점 돌연변이 유전자에 높은 민감도 및 특이도를 가지며, 기존의 표적 돌연변이 유전자 검출방법에 비해 검출의 정확성을 높일 수 있다.
PCT/KR2018/004700 2017-04-21 2018-04-23 표적 점 돌연변이 유전자 검출을 위한 핵산 분자 템플레이트 및 이를 이용한 유전자 검사방법 WO2018194437A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0051546 2017-04-21
KR20170051546 2017-04-21

Publications (2)

Publication Number Publication Date
WO2018194437A2 WO2018194437A2 (ko) 2018-10-25
WO2018194437A3 true WO2018194437A3 (ko) 2019-02-28

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PCT/KR2018/004700 WO2018194437A2 (ko) 2017-04-21 2018-04-23 표적 점 돌연변이 유전자 검출을 위한 핵산 분자 템플레이트 및 이를 이용한 유전자 검사방법

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KR (1) KR102330252B1 (ko)
WO (1) WO2018194437A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110343745A (zh) * 2019-06-09 2019-10-18 陈超 一种egfr/l858r突变超敏检测试剂盒
KR102292111B1 (ko) * 2019-10-18 2021-08-20 한국식품연구원 수은 검출을 위한 현장 진단 장치 및 이를 이용하는 수은 검출 방법
KR102300178B1 (ko) * 2019-11-08 2021-09-09 고려대학교 산학협력단 중합효소연쇄반응 및 dna 라이게이션 반응을 기반으로 한 표적 점 돌연변이의 검출 방법

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR950013953B1 (ko) * 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
US6344329B1 (en) * 1995-11-21 2002-02-05 Yale University Rolling circle replication reporter systems
CN102220413B (zh) * 2011-03-08 2013-05-08 中国科学院北京基因组研究所 检测表皮生长因子受体基因外显子19缺失突变和外显子21点突变的方法
US8679788B2 (en) * 2004-03-22 2014-03-25 The Johns Hopkins University Methods for the detection of nucleic acid differences
KR20160052400A (ko) * 2014-10-30 2016-05-12 이화여자대학교 산학협력단 표적 유전자 검출용 미세 유동 장치, 이의 제조방법 및 이를 이용한 검출 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013173774A2 (en) * 2012-05-18 2013-11-21 Pathogenica, Inc. Molecular inversion probes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR950013953B1 (ko) * 1990-01-26 1995-11-18 애보트 래보라토리즈 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법
US6344329B1 (en) * 1995-11-21 2002-02-05 Yale University Rolling circle replication reporter systems
US8679788B2 (en) * 2004-03-22 2014-03-25 The Johns Hopkins University Methods for the detection of nucleic acid differences
CN102220413B (zh) * 2011-03-08 2013-05-08 中国科学院北京基因组研究所 检测表皮生长因子受体基因外显子19缺失突变和外显子21点突变的方法
KR20160052400A (ko) * 2014-10-30 2016-05-12 이화여자대학교 산학협력단 표적 유전자 검출용 미세 유동 장치, 이의 제조방법 및 이를 이용한 검출 방법

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KR20180118561A (ko) 2018-10-31
KR102330252B1 (ko) 2021-11-24
WO2018194437A2 (ko) 2018-10-25

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