MX2020003793A - Medios de diagnostico para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra. - Google Patents

Medios de diagnostico para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra.

Info

Publication number
MX2020003793A
MX2020003793A MX2020003793A MX2020003793A MX2020003793A MX 2020003793 A MX2020003793 A MX 2020003793A MX 2020003793 A MX2020003793 A MX 2020003793A MX 2020003793 A MX2020003793 A MX 2020003793A MX 2020003793 A MX2020003793 A MX 2020003793A
Authority
MX
Mexico
Prior art keywords
sample
analytes present
diagnostic means
quantification
detection
Prior art date
Application number
MX2020003793A
Other languages
English (en)
Inventor
Vincent Chabottaux
Thomas Glouden
Benoit Granier
Original Assignee
Unisensor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from BE2017/5707A external-priority patent/BE1025623B1/fr
Priority claimed from BE2017/5705A external-priority patent/BE1025621B1/fr
Priority claimed from BE2017/5709A external-priority patent/BE1025616B1/fr
Priority claimed from BE2017/5708A external-priority patent/BE1025624B1/fr
Priority claimed from BE2017/5706A external-priority patent/BE1025622B1/fr
Application filed by Unisensor filed Critical Unisensor
Publication of MX2020003793A publication Critical patent/MX2020003793A/es

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/06Investigating concentration of particle suspensions
    • G01N15/0606Investigating concentration of particle suspensions by collecting particles on a support
    • G01N15/0612Optical scan of the deposits
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • G01N33/54387Immunochromatographic test strips
    • G01N33/54388Immunochromatographic test strips based on lateral flow
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446Antibacterials
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7756Sensor type
    • G01N2021/7759Dipstick; Test strip
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251Colorimeters; Construction thereof
    • G01N21/253Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • G01N21/8483Investigating reagent band
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/06Illumination; Optics
    • G01N2201/069Supply of sources
    • G01N2201/0692Regulated sources; stabilised supply
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/06Illumination; Optics
    • G01N2201/069Supply of sources
    • G01N2201/0695Supply to maintain constant beam intensity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Medios de diagnóstico inmunocromatografico (1) para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra esencialmente liquida (E), que comprende: - al menos una mezcla de reacción (2) que contiene moléculas biológicas de reconocimiento y/o ligandos competitivos marcados con al menos una molécula de visualización que es detectable en fluorescencia, estando dicha mezcla de reacción presente en un recipiente separado de dicho sistema de recuperación (3); y - al menos un sistema de recuperación (3) en forma de un soporte solido al que se unen ligandos competitivos y/o moléculas biológicas de reconocimiento en ubicaciones de recuperación distintas y conocidas (4 y 5), que están dispuestas de acuerdo con una disposición de matriz bidimensional definida de acuerdo con un sistema de coordenadas, para identificar mediante la localización de dichas ubicaciones de recuperación (4 y 5) en dicho soporte, dichos analitos presentes en dicha muestra (E).
MX2020003793A 2017-10-04 2018-10-04 Medios de diagnostico para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra. MX2020003793A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
BE2017/5707A BE1025623B1 (fr) 2017-10-04 2017-10-04 Dispositif de lecture optique à la carte d’un support solide amovible pour la détection et/ou la quantification d’analytes présents dans un échantillon
BE2017/5705A BE1025621B1 (fr) 2017-10-04 2017-10-04 Dispositif de lecture optique à intensité de lumière contrôlée d’un support solide amovible pour la détection et/ou la quantification d’analytes présents dans un échantillon
BE2017/5709A BE1025616B1 (fr) 2017-10-04 2017-10-04 Moyen de diagnostic pour la détection et/ou la quantification d’une pluralité d’analytes présents dans un échantillon
BE2017/5708A BE1025624B1 (fr) 2017-10-04 2017-10-04 Dispositif de lecture optique avec choix de méthode de lecture automatisé d’un support solide amovible pour la détection et/ou la quantification d’analytes présents dans un échantillon
BE2017/5706A BE1025622B1 (fr) 2017-10-04 2017-10-04 Dispositif de lecture optique matricielle d’un support solide amovible pour la détection et/ou la quantification d’analytes présents dans un échantillon
PCT/EP2018/076993 WO2019068806A1 (fr) 2017-10-04 2018-10-04 Moyen de diagnostic pour la détection et/ou la quantification d'une pluralité d'analytes présents dans un échantillon

Publications (1)

Publication Number Publication Date
MX2020003793A true MX2020003793A (es) 2020-11-09

Family

ID=63720707

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020003793A MX2020003793A (es) 2017-10-04 2018-10-04 Medios de diagnostico para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra.

Country Status (9)

Country Link
US (2) US20200256797A1 (es)
EP (1) EP3692367A1 (es)
JP (1) JP7361026B2 (es)
CN (1) CN111512157A (es)
AU (1) AU2018346295A1 (es)
BR (1) BR112020006581A2 (es)
CA (1) CA3078150A1 (es)
MX (1) MX2020003793A (es)
WO (3) WO2019068802A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021194635A1 (en) * 2020-03-24 2021-09-30 Mcgrew Stephen P Combinatorial affinity-based analysis assemblies and methods
BE1029224B1 (fr) 2021-03-22 2022-10-17 Unisensor Dispositif de pilotage
BE1029228B1 (fr) 2021-03-22 2022-10-18 Unisensor Dispositif microfluidique
WO2022200270A1 (fr) 2021-03-22 2022-09-29 Unisensor Dispositif de pilotage
BE1029227B1 (fr) 2021-03-22 2022-10-17 Unisensor Dispositif microfluidique rotatif

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6800452B1 (en) * 1994-08-08 2004-10-05 Science Applications International Corporation Automated methods for simultaneously performing a plurality of signal-based assays
CN1138981C (zh) * 2000-04-27 2004-02-18 赵翀 一种高信息量免疫检测方法及其专用检测板
CA2422224A1 (en) 2002-03-15 2003-09-15 Affymetrix, Inc. System, method, and product for scanning of biological materials
US7682782B2 (en) * 2004-10-29 2010-03-23 Affymetrix, Inc. System, method, and product for multiple wavelength detection using single source excitation
US20080003599A1 (en) * 2004-12-28 2008-01-03 Dary Ekaterina L Biological Microchip for Multiple Parallel Immunoassay of Compounds and Immunoassay Metods Using Said Microchip
EP1866653A4 (en) * 2005-03-07 2009-08-26 Novx Systems Inc ANALYSIS MACHINE
EP1712914A1 (fr) 2005-04-14 2006-10-18 Unisensor S.A. Procédé in vitro pour la detection et l'identification simultanées d'antibiotiques de classes differentes et trousse de diagnostic correspondante
EP2294408A4 (en) * 2008-02-21 2011-05-25 Gentel Biosciences Inc SUBSTRATES FOR MULTIPLEX TEST PROCEDURES AND USES THEREFOR
US8446463B2 (en) * 2008-08-22 2013-05-21 Genprime, Inc. Apparatus, method and article to perform assays using assay strips
US20110244597A1 (en) * 2008-11-28 2011-10-06 Konica Minolta Medical & Graphic, Inc. Immunochromatographic medium and immunochromatographic method
US9658222B2 (en) * 2009-03-02 2017-05-23 Mbio Diagnostics, Inc. Planar waveguide based cartridges and associated methods for detecting target analyte
US9212995B2 (en) * 2009-03-02 2015-12-15 Mbio Diagnostics, Inc. System and method for detecting multiple molecules in one assay
CA2684636A1 (en) * 2009-10-30 2011-04-30 Sqi Diagnostics Systems Inc Multiplex microarrays and methods for the quantification of analytes
WO2012012499A1 (en) 2010-07-20 2012-01-26 Nurx Pharmaceuticals, Inc. Optical reader system
US8486717B2 (en) * 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
US9874556B2 (en) * 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
WO2014055559A1 (en) * 2012-10-01 2014-04-10 The Trustees Of The Princeton University Microfluidic sensors with enhanced optical signals
WO2014150853A1 (en) * 2013-03-15 2014-09-25 Inanovate, Inc. Analyte measurement using longitudinal assay
US11352657B2 (en) * 2015-03-05 2022-06-07 Duke University Glucose/galactose biosensors and methods of using same
US20180319657A1 (en) * 2015-11-04 2018-11-08 Biocifer Pty Ltd Multiplex Lateral Flow Devices and Assays

Also Published As

Publication number Publication date
WO2019068804A1 (fr) 2019-04-11
BR112020006581A2 (pt) 2020-10-06
WO2019068802A1 (fr) 2019-04-11
US20240077422A1 (en) 2024-03-07
CN111512157A (zh) 2020-08-07
WO2019068806A1 (fr) 2019-04-11
JP2020536254A (ja) 2020-12-10
CA3078150A1 (en) 2019-04-11
US20200256797A1 (en) 2020-08-13
EP3692367A1 (fr) 2020-08-12
AU2018346295A1 (en) 2020-04-23
RU2020112945A3 (es) 2022-02-10
RU2020112945A (ru) 2021-11-08
JP7361026B2 (ja) 2023-10-13

Similar Documents

Publication Publication Date Title
MX2020003793A (es) Medios de diagnostico para detectar y/o cuantificar una pluralidad de analitos presentes en una muestra.
ES2559108T3 (es) Método de detección de nucleótidos simples
IN2014DN08135A (es)
BR112018071543A2 (pt) execução do teste de suscetibilidade antimicrobiana e sistemas e métodos relacionados
RU2015141509A (ru) Способы и средства для диагностики гриппа
GB201216465D0 (en) Immobilised-bead immunomultiplex assay
JP2016524127A5 (es)
CN206715966U (zh) 一种生物分析用微流控检测装置
WO2020204674A3 (ko) Cfdna를 이용한 암 진단방법
EP3404410A3 (en) Biosensor system for the rapid detection of analytes
Jamiruddin et al. Microfluidics technology in SARS-CoV-2 diagnosis and beyond: a systematic review
Cui et al. A volumetric meter chip for point-of-care quantitative detection of bovine catalase for food safety control
SG10201908265UA (en) L-fabp immunoassay method and assay reagent used in said method
RU2537468C2 (ru) Способ определения маркеров транспортируемых нефти и нефтепродуктов и устройство для его осуществления
ES2640570T3 (es) Ensayo de doble sonda para la detección de VHC
CN103954753A (zh) 一种免疫层析试纸条的定量检测方法
CN105301131A (zh) 一种使用upc2-elsd测定分子蒸馏单硬脂酸甘油酯中单甘酯的方法
Okochi et al. Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system
BR112017016798A2 (pt) métodos e sistemas para determinar regiões ópticas de interesse
Zarzycki Detection and Analysis of Microbes, Bioanalytes, and Micropollutants, Focusing on Food and Environmental Samples, Using Nanoparticle-Based Detection Systems, Microfluidic Analytical Devices, and Related Techniques.
CN105044356A (zh) 一种新型前列腺特异性抗原检测试剂盒
Sailis Detection of miRNA by SMART technology
Hanna-Brown Pharmaceutical Analysis
Hoekstra et al. Sub-base-pair Resolution by Electrophoretic Separation and Fluorescent Detection of DNA Molecules in an Optofluidic Chip
Joo et al. Single molecule protein sequencing