MX2018011534A - Metodos para transfectar plantas y para reducir eventos de integracion aleatoria. - Google Patents

Metodos para transfectar plantas y para reducir eventos de integracion aleatoria.

Info

Publication number
MX2018011534A
MX2018011534A MX2018011534A MX2018011534A MX2018011534A MX 2018011534 A MX2018011534 A MX 2018011534A MX 2018011534 A MX2018011534 A MX 2018011534A MX 2018011534 A MX2018011534 A MX 2018011534A MX 2018011534 A MX2018011534 A MX 2018011534A
Authority
MX
Mexico
Prior art keywords
plants
methods
random integration
integration events
reducing random
Prior art date
Application number
MX2018011534A
Other languages
English (en)
Inventor
Tijsterman Marcel
Van Kregten Maartje
Hooykaas Paul
Original Assignee
Univ Leiden
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leiden filed Critical Univ Leiden
Publication of MX2018011534A publication Critical patent/MX2018011534A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente descripción proporciona métodos para transfectar plantas y para expresar moléculas de ARN o de polipéptido en plantas. En particular, las plantas que tienen una expresión y/o actividad de POLQ reducidas son transfectadas con el fin de reducir los eventos de integración aleatoria. La descripción proporciona además plantas transfectadas y progenie de plantas producidas mediante los métodos descritos en la presente.
MX2018011534A 2016-03-24 2017-03-23 Metodos para transfectar plantas y para reducir eventos de integracion aleatoria. MX2018011534A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16162322 2016-03-24
PCT/NL2017/050182 WO2017164738A1 (en) 2016-03-24 2017-03-23 Methods for transfecting plants and for reducing random integration events

Publications (1)

Publication Number Publication Date
MX2018011534A true MX2018011534A (es) 2019-07-04

Family

ID=55628927

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018011534A MX2018011534A (es) 2016-03-24 2017-03-23 Metodos para transfectar plantas y para reducir eventos de integracion aleatoria.

Country Status (15)

Country Link
US (1) US11525140B2 (es)
EP (1) EP3433367B1 (es)
JP (1) JP2019509053A (es)
KR (1) KR20190003504A (es)
CN (1) CN109196105B (es)
AR (1) AR109117A1 (es)
AU (1) AU2017237643B2 (es)
BR (1) BR112018069506A2 (es)
CA (1) CA3018306A1 (es)
EA (1) EA039928B1 (es)
ES (1) ES2892873T3 (es)
MX (1) MX2018011534A (es)
UA (1) UA127447C2 (es)
WO (1) WO2017164738A1 (es)
ZA (1) ZA201806572B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
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CN109196105B (zh) 2016-03-24 2022-08-05 莱顿大学 转染植物和减少随机整合事件的方法
JP6829306B2 (ja) * 2017-03-29 2021-02-10 パイオニア株式会社 移動体装置、端末装置、情報処理システム及び情報処理方法並びに移動体装置用プログラム及び端末装置用プログラム
BR112020008386A2 (pt) * 2017-10-30 2020-11-03 KWS SAAT SE & Co. KGaA estratégias para edição de genoma de precisão
EP3502259A1 (en) * 2017-12-19 2019-06-26 Universiteit Leiden A combinational strategy for reducing random integration events when transfecting plants
WO2019234132A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Base editing in polymerase theta deficient plants
WO2019234129A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
EP3660164A1 (en) 2018-11-30 2020-06-03 KWS SAAT SE & Co. KGaA Screening of germplasm for desired polymorphisms by assays tested on synthetic dna
CN113881703B (zh) * 2021-10-11 2022-06-21 中国人民解放军军事科学院军事医学研究院 一种提高cho细胞同源重组效率的方法及其相关产品和应用

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NL8401780A (nl) 1984-06-04 1986-01-02 Rijksuniversiteit Leiden En Pr Werkwijze voor het inbouwen van vreemd dna in het genoom van planten.
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
EP0837624A1 (en) 1995-06-30 1998-04-29 DNA Plant Technology Corporation Delayed ripening tomato plants
GB9703146D0 (en) 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
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US6452067B1 (en) 1997-09-19 2002-09-17 Dna Plant Technology Corporation Methods to assay for post-transcriptional suppression of gene expression
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
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Also Published As

Publication number Publication date
ES2892873T3 (es) 2022-02-07
WO2017164738A1 (en) 2017-09-28
UA127447C2 (uk) 2023-08-30
JP2019509053A (ja) 2019-04-04
BR112018069506A2 (pt) 2019-01-29
AU2017237643A1 (en) 2018-10-11
AU2017237643B2 (en) 2023-06-22
EP3433367B1 (en) 2021-07-14
KR20190003504A (ko) 2019-01-09
CN109196105B (zh) 2022-08-05
EA201891919A1 (ru) 2019-04-30
US20190390208A1 (en) 2019-12-26
US11525140B2 (en) 2022-12-13
CN109196105A (zh) 2019-01-11
CA3018306A1 (en) 2017-09-28
EP3433367A1 (en) 2019-01-30
ZA201806572B (en) 2021-02-24
EA039928B1 (ru) 2022-03-29
AR109117A1 (es) 2018-10-31

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