MX2017014516A - Metodo para la elucion continua de un producto de columnas de cromatografia. - Google Patents

Metodo para la elucion continua de un producto de columnas de cromatografia.

Info

Publication number
MX2017014516A
MX2017014516A MX2017014516A MX2017014516A MX2017014516A MX 2017014516 A MX2017014516 A MX 2017014516A MX 2017014516 A MX2017014516 A MX 2017014516A MX 2017014516 A MX2017014516 A MX 2017014516A MX 2017014516 A MX2017014516 A MX 2017014516A
Authority
MX
Mexico
Prior art keywords
time
loading
product
chromatography columns
column
Prior art date
Application number
MX2017014516A
Other languages
English (en)
Inventor
Baumarth Kerstin
Lobedann Martin
Schwan Peter
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Publication of MX2017014516A publication Critical patent/MX2017014516A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La invención ofrece un método para la elución continua de un producto biológico macromolecular biofarmacéutico proveniente de más de una columna de cromatografía, que comprende los siguientes pasos: (a) disponer una corriente de producto por una entrada, (b) cargar simultáneamente n columnas de cromatografía frontal con la corriente de producto en una zona de carga con un tiempo de carga tB, donde una corriente de salida de las n columnas de cromatografía frontal es distribuida simultáneamente entre al menos n-1 columnas de cromatografía de recuperación en una zona de recuperación, donde n es entre 1 y 5, y donde las n columnas de cromatografía frontal presentan en un momento dado, un tiempo de carga tB de duración diferente, de entre 0 y L1, entre L1 y L2, y hasta de entre Ln-1 y Ln, donde cada columna de cromatografía frontal presenta una tiempo de carga total de Ln, caracterizado porque, después de un tiempo de cambio ts constante (Switch Time) de Ln - Ln-1, periódicamente la n columna de cromatografía frontal sale de la zona de carga y una columna de cromatografía de recuperación de la zona de recuperación entra en la zona de carga, (c) lavado de la columna cargada de producto del paso b) con al menos una solución amortiguadora de lavado, (d) elución del producto de la columna lavada del paso c) con un tiempo de elución tE, que es = 80% que el tiempo de cambio tS (Switch-Time), y porque al menos una columna de cromatografía se encuentra en el paso de elución (d) de manera continua más del 80% del tiempo operativo total tG.
MX2017014516A 2015-05-13 2016-05-10 Metodo para la elucion continua de un producto de columnas de cromatografia. MX2017014516A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15167518.8A EP3037513A1 (de) 2015-05-13 2015-05-13 Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen
PCT/EP2016/060368 WO2016180797A1 (de) 2015-05-13 2016-05-10 Verfahren zur kontinuierlichen elution eines produktes von chromatographiesäulen

Publications (1)

Publication Number Publication Date
MX2017014516A true MX2017014516A (es) 2018-03-15

Family

ID=53177194

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017014516A MX2017014516A (es) 2015-05-13 2016-05-10 Metodo para la elucion continua de un producto de columnas de cromatografia.

Country Status (16)

Country Link
US (1) US20180117495A1 (es)
EP (2) EP3037513A1 (es)
JP (1) JP6676073B2 (es)
KR (1) KR20180004726A (es)
CN (1) CN107683285A (es)
AR (1) AR104573A1 (es)
AU (1) AU2016259745B2 (es)
BR (1) BR112017024339A2 (es)
CA (1) CA2985679A1 (es)
HK (1) HK1244822A1 (es)
IL (1) IL255434A0 (es)
MX (1) MX2017014516A (es)
RU (1) RU2017143436A (es)
SG (1) SG11201709192RA (es)
TW (1) TWI679053B (es)
WO (1) WO2016180797A1 (es)

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US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
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Also Published As

Publication number Publication date
SG11201709192RA (en) 2017-12-28
AU2016259745B2 (en) 2020-08-06
RU2017143436A (ru) 2019-06-17
TWI679053B (zh) 2019-12-11
BR112017024339A2 (pt) 2018-07-31
JP2018524146A (ja) 2018-08-30
WO2016180797A1 (de) 2016-11-17
IL255434A0 (en) 2017-12-31
AU2016259745A1 (en) 2017-11-30
TW201709962A (zh) 2017-03-16
EP3037513A1 (de) 2016-06-29
US20180117495A1 (en) 2018-05-03
JP6676073B2 (ja) 2020-04-08
HK1244822A1 (zh) 2018-08-17
KR20180004726A (ko) 2018-01-12
EP3294857A1 (de) 2018-03-21
RU2017143436A3 (es) 2019-11-28
AR104573A1 (es) 2017-08-02
CA2985679A1 (en) 2016-11-17
CN107683285A (zh) 2018-02-09

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