MX2014003326A - Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman. - Google Patents

Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman.

Info

Publication number
MX2014003326A
MX2014003326A MX2014003326A MX2014003326A MX2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A MX 2014003326 A MX2014003326 A MX 2014003326A
Authority
MX
Mexico
Prior art keywords
combination
fab fragments
producing cells
antibody producing
single antibody
Prior art date
Application number
MX2014003326A
Other languages
English (en)
Inventor
Valeria Lifke
Hans-Willi Krell
Alexander Lifke
Kairat Madin
Christian Weilke
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014003326A publication Critical patent/MX2014003326A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En la presente se reporta un método para reacción en cadena de polimerasa específica de gen, por transcriptasa inversa en tiempo real en un tubo, multiplexada para amplificación y cuantificación de ácidos nucleicos que codifican para las cadenas pesada y ligera de IgG a fin (isotipo de IgG humano) a partir de una célula única.
MX2014003326A 2011-09-21 2012-09-20 Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman. MX2014003326A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11182223 2011-09-21
PCT/EP2012/068532 WO2013041617A1 (en) 2011-09-21 2012-09-20 METHOD FOR OBTAINING FAB FRAGMENTS FROM SINGLE ANTIBODY PRODUCING CELLS BY MULTIPLEXED PCR IN COMBINATION WITH TaqMan PROBES

Publications (1)

Publication Number Publication Date
MX2014003326A true MX2014003326A (es) 2014-04-25

Family

ID=46852037

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014003326A MX2014003326A (es) 2011-09-21 2012-09-20 Metodo para obtener fragmentos de fab a partir de celulas unicas productoras de un anticuerpo mediante pcr multiplexado en combinacion con sondas taqman.

Country Status (11)

Country Link
US (1) US20150024434A1 (es)
EP (1) EP2758428A1 (es)
JP (1) JP2014527825A (es)
KR (1) KR20140064857A (es)
CN (1) CN103814047A (es)
BR (1) BR112014004566A2 (es)
CA (1) CA2844838A1 (es)
HK (1) HK1198169A1 (es)
MX (1) MX2014003326A (es)
RU (1) RU2014113678A (es)
WO (1) WO2013041617A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021226917A1 (en) * 2020-05-14 2021-11-18 Singleron (Nanjing) Biotechnologies, Ltd. Novel method of one-step whole transcriptome amplification

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU600575B2 (en) 1987-03-18 1990-08-16 Sb2, Inc. Altered antibodies
US5204244A (en) 1987-10-27 1993-04-20 Oncogen Production of chimeric antibodies by homologous recombination
US5202238A (en) 1987-10-27 1993-04-13 Oncogen Production of chimeric antibodies by homologous recombination
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US5571690A (en) 1991-05-08 1996-11-05 The University Of Virginia Patents Foundation Method for the cell-free synthesis of proteins
GB9613418D0 (en) 1996-06-26 1996-08-28 Crc Technology Ltd A cell-free system for initiation of DNA replication
AU5337198A (en) 1997-01-21 1998-08-07 Daniel Favre Production of biologically active polypeptides
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
CA2365668C (en) 1999-03-17 2014-05-20 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
EP1309347B1 (en) 2000-08-11 2013-02-27 Mmrglobal, Inc. Method and composition for altering a b cell mediated pathology
EP1678327A4 (en) * 2003-10-16 2007-10-10 Genomic Health Inc QRT-PCR TEST SYSTEM FOR GENE EXPRESSION PROFILING
EP2121920B1 (en) 2007-03-01 2011-08-24 Symphogen A/S Method for cloning cognate antibodies
US9399670B2 (en) * 2009-02-20 2016-07-26 Hoffmann-La Roche Inc. Method for obtaining immunoglobulin encoding nucleic acid
US20130029340A1 (en) * 2010-01-15 2013-01-31 Steffen Mergemeier Method for detecting more than one target in a pcr-based approach applying an un-specific dye which is not interfering with the emission of fluorophore-labeled probes

Also Published As

Publication number Publication date
WO2013041617A1 (en) 2013-03-28
EP2758428A1 (en) 2014-07-30
RU2014113678A (ru) 2015-10-27
JP2014527825A (ja) 2014-10-23
CA2844838A1 (en) 2013-03-28
HK1198169A1 (en) 2015-03-13
CN103814047A (zh) 2014-05-21
US20150024434A1 (en) 2015-01-22
BR112014004566A2 (pt) 2017-06-13
KR20140064857A (ko) 2014-05-28

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