MX2012002734A - Metodo mejorado para la cuantificacion de acido nucleico. - Google Patents

Metodo mejorado para la cuantificacion de acido nucleico.

Info

Publication number
MX2012002734A
MX2012002734A MX2012002734A MX2012002734A MX2012002734A MX 2012002734 A MX2012002734 A MX 2012002734A MX 2012002734 A MX2012002734 A MX 2012002734A MX 2012002734 A MX2012002734 A MX 2012002734A MX 2012002734 A MX2012002734 A MX 2012002734A
Authority
MX
Mexico
Prior art keywords
nucleic acid
quantitation method
improved nucleic
acid quantitation
nucleic acids
Prior art date
Application number
MX2012002734A
Other languages
English (en)
Inventor
Karen Annette Duggan
Hong Ha
George Hodge
Original Assignee
Accugen Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2009904258A external-priority patent/AU2009904258A0/en
Application filed by Accugen Pty Ltd filed Critical Accugen Pty Ltd
Publication of MX2012002734A publication Critical patent/MX2012002734A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención describe métodos para cuantificar ácidos nucleicos y en particular a un método universal mejorado para cuantificar ácidos nucleicos para estudios de expresión de gen sin la necesidad de normalizar datos a un gen doméstico o a un gen sintético de interés.
MX2012002734A 2009-09-02 2010-09-02 Metodo mejorado para la cuantificacion de acido nucleico. MX2012002734A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2009904258A AU2009904258A0 (en) 2009-09-02 Improved quantitation method
PCT/AU2010/001131 WO2011026182A1 (en) 2009-09-02 2010-09-02 Improved nucleic acid quantitation method

Publications (1)

Publication Number Publication Date
MX2012002734A true MX2012002734A (es) 2012-07-20

Family

ID=43648774

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012002734A MX2012002734A (es) 2009-09-02 2010-09-02 Metodo mejorado para la cuantificacion de acido nucleico.

Country Status (20)

Country Link
US (1) US9238831B2 (es)
EP (1) EP2473632B1 (es)
JP (1) JP5870030B2 (es)
KR (4) KR20170051544A (es)
CN (1) CN102725421B (es)
AU (1) AU2010291867B2 (es)
BR (1) BR112012004788B1 (es)
CA (1) CA2772770C (es)
CL (1) CL2012000569A1 (es)
DK (1) DK2473632T3 (es)
ES (1) ES2665763T3 (es)
IL (1) IL218423A (es)
MX (1) MX2012002734A (es)
MY (1) MY159569A (es)
NZ (1) NZ598876A (es)
PL (1) PL2473632T3 (es)
RU (1) RU2562161C2 (es)
SG (1) SG178960A1 (es)
WO (1) WO2011026182A1 (es)
ZA (1) ZA201202210B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017007733A2 (pt) * 2014-10-17 2018-01-30 Accugen Pty Ltd método para quantificação de um ácido nucleico alvo, e, kit.
RU2631935C1 (ru) * 2016-08-04 2017-09-28 Федеральное казенное учреждение здравоохранения Волгоградский научно-исследовательский противочумный институт Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Набор олигонуклеотидных праймеров для идентификации медицински значимых микромицетов методом секвенирования днк
CN114341627A (zh) * 2019-11-13 2022-04-12 李峰 核酸检测方法及装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7235358B2 (en) * 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
CN100338227C (zh) * 2005-04-20 2007-09-19 浙江大学医学院附属妇产科医院 实时定量pcr技术检测人21号染色体数目的方法
US20070122818A1 (en) * 2005-10-31 2007-05-31 Abhijit Mazumder Gene methylation assay controls
US20110008328A1 (en) * 2007-04-17 2011-01-13 Pfizer Inc. Method for controlling glucose uptake and insulin sensitivity

Also Published As

Publication number Publication date
CN102725421B (zh) 2018-04-10
BR112012004788B1 (pt) 2021-02-09
KR20170051544A (ko) 2017-05-11
IL218423A (en) 2016-07-31
DK2473632T3 (en) 2018-07-16
ZA201202210B (en) 2014-06-25
RU2012111733A (ru) 2013-10-10
CA2772770C (en) 2019-01-15
JP5870030B2 (ja) 2016-02-24
RU2562161C2 (ru) 2015-09-10
SG178960A1 (en) 2012-04-27
EP2473632A4 (en) 2013-07-17
WO2011026182A1 (en) 2011-03-10
EP2473632A1 (en) 2012-07-11
US20120231465A1 (en) 2012-09-13
AU2010291867A1 (en) 2012-04-12
IL218423A0 (en) 2012-04-30
KR20120100902A (ko) 2012-09-12
MY159569A (en) 2017-01-13
US9238831B2 (en) 2016-01-19
KR20150104639A (ko) 2015-09-15
CN102725421A (zh) 2012-10-10
EP2473632B1 (en) 2018-04-04
CA2772770A1 (en) 2011-03-10
NZ598876A (en) 2014-07-25
KR20160092522A (ko) 2016-08-04
ES2665763T3 (es) 2018-04-27
BR112012004788A2 (pt) 2020-02-04
AU2010291867B2 (en) 2014-08-14
CL2012000569A1 (es) 2012-11-23
JP2013503608A (ja) 2013-02-04
PL2473632T3 (pl) 2018-08-31

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