MX2009008060A - Un metodo de multiples parametros de sensibilidad alta para el analisis de eventos raros en una muestra biologica. - Google Patents

Un metodo de multiples parametros de sensibilidad alta para el analisis de eventos raros en una muestra biologica.

Info

Publication number
MX2009008060A
MX2009008060A MX2009008060A MX2009008060A MX2009008060A MX 2009008060 A MX2009008060 A MX 2009008060A MX 2009008060 A MX2009008060 A MX 2009008060A MX 2009008060 A MX2009008060 A MX 2009008060A MX 2009008060 A MX2009008060 A MX 2009008060A
Authority
MX
Mexico
Prior art keywords
cells
interest
sample
targets
circulating
Prior art date
Application number
MX2009008060A
Other languages
English (en)
Inventor
Mark Carle Connelly
Frank Coumans
Steve Gross
James Michael Kelly
Original Assignee
Veridex Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41611032&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2009008060(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Veridex Llc filed Critical Veridex Llc
Publication of MX2009008060A publication Critical patent/MX2009008060A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Ecology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Una muestra de sangre contiene CTC, u otras células de interés, se tiñe con marcadores fluorescentes para el análisis de imagen y se escanea para identificar la presencia y ubicación dentro del cartucho de células objetivo o elementos subcelulares; una muestra que contiene células objetivo deseadas o elementos subcelulares entonces se procesa adicionalmente, en parte por medio del fotoblanqueado de la muestra, para que aquellos mismos objetivos puedan reanalizarse con biomarcadores adicionales conjugados con los mismos o diferentes fluorocromos utilizando los mismos criterios de imágenes que se utilizaron para el análisis inicial, esta invención tiene aplicaciones con objetivos tales como células epiteliales circulantes, células tumorales circulantes, células endoteliales circulantes, leucocitos, subjuegos de linfocitos, células que contienen un organelo o receptor de interés, residuos celulares, células fraccionadas y sus residuos o cualquier otro elemento formado que pudiera capturarse y tomar la imagen; la invención provee un medio para indagar adicionalmente sobre objetivos individuales de interés, especialmente cuando se acoplan con el análisis genético tal como FISH.
MX2009008060A 2008-07-29 2009-07-29 Un metodo de multiples parametros de sensibilidad alta para el analisis de eventos raros en una muestra biologica. MX2009008060A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US12/181,399 US9134237B2 (en) 2005-09-20 2008-07-29 High sensitivity multiparameter method for rare event analysis in a biological sample

Publications (1)

Publication Number Publication Date
MX2009008060A true MX2009008060A (es) 2010-07-01

Family

ID=41611032

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009008060A MX2009008060A (es) 2008-07-29 2009-07-29 Un metodo de multiples parametros de sensibilidad alta para el analisis de eventos raros en una muestra biologica.

Country Status (15)

Country Link
US (1) US9134237B2 (es)
EP (1) EP2169387B1 (es)
JP (1) JP6109464B2 (es)
KR (2) KR101764597B1 (es)
CN (1) CN101672779B (es)
BR (2) BRPI0902559A2 (es)
CA (2) CA2975186A1 (es)
DK (1) DK2169387T3 (es)
ES (1) ES2542061T3 (es)
HK (1) HK1140820A1 (es)
HU (1) HUE026779T2 (es)
IL (1) IL199798A (es)
MX (1) MX2009008060A (es)
PL (1) PL2169387T3 (es)
PT (1) PT2169387E (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2886923T3 (es) 2005-09-20 2021-12-21 Menarini Silicon Biosystems Spa Métodos y composición para generar sondas de ADN de secuencia única, marcaje de sondas de ADN y el uso de estas sondas
WO2011050103A1 (en) 2009-10-21 2011-04-28 The Scripps Research Institute Method of using non-rare cells to detect rare cells
DK2626431T3 (en) 2010-10-06 2015-12-21 Fundació Inst De Recerca Biomèdica Irb Barcelona A process for the diagnosis, prognosis and treatment of breast cancer metastasis
US10114020B2 (en) 2010-10-11 2018-10-30 Mbio Diagnostics, Inc. System and device for analyzing a fluidic sample
CN103765194B (zh) * 2011-08-30 2016-02-17 奥林巴斯株式会社 目标粒子的检测方法
WO2013072571A1 (fr) * 2011-11-17 2013-05-23 Institut Gustave Roussy Methode de caracterisation de cellules tumorales circulantes et application au diagnostic
EP2650682A1 (en) 2012-04-09 2013-10-16 Fundació Privada Institut de Recerca Biomèdica Method for the prognosis and treatment of cancer metastasis
MX368513B (es) 2012-06-06 2019-10-07 Fundacio Inst De Recerca Biomedica Irb Barcelona Métodos in vitro para el pronóstico, diagnóstico y diseño de una terapia de metástasis de cáncer de pulmón.
US10119171B2 (en) 2012-10-12 2018-11-06 Inbiomotion S.L. Method for the diagnosis, prognosis and treatment of prostate cancer metastasis
WO2014057357A2 (en) 2012-10-12 2014-04-17 Inbiomotion S.L. Method for the diagnosis, prognosis and treatment of prostate cancer metastasis
EP2911791A4 (en) 2012-10-29 2016-11-02 Mbio Diagnostics Inc SYSTEM FOR IDENTIFYING BIOLOGICAL PARTICLES, CARTRIDGE AND ASSOCIATED METHODS
EA201691496A1 (ru) 2014-01-27 2016-12-30 Эпик Сайенсиз, Инк. Диагностика биомаркеров рака предстательной железы с помощью циркулирующих опухолевых клеток
EP3108246B1 (en) * 2014-02-21 2019-10-09 Epic Sciences, Inc. Methods for analyzing rare circulating cells
CN107109477B (zh) * 2014-08-25 2021-01-12 创新微技术公司 在血液中的循环细胞生物标记在检测和诊断疾病中的应用及其分离方法
EP3229909B1 (en) 2014-12-11 2020-10-14 Inbiomotion S.L. Binding members for human c-maf
WO2016115537A2 (en) * 2015-01-15 2016-07-21 Massachusetts Institute Of Technology Systems, methods, and apparatus for in vitro single-cell identification and recovery
GB201507202D0 (en) * 2015-04-28 2015-06-10 Stfc Science & Technology Receptor tyosine kinase biomarkers
EP3400313A4 (en) * 2016-01-07 2019-09-18 Creatv Microtech, Inc. MULTI-PHENOTYPIC SUBTYPIZATION OF BIOLOGICAL SAMPLES THROUGH SEQUENCING FLUORESCENCE DELETION AND RENEWING
AU2017271385B2 (en) 2016-05-25 2023-10-05 Inbiomotion S.L. Therapeutic treatment of breast cancer based on c-MAF status
US11654153B2 (en) 2017-11-22 2023-05-23 Inbiomotion S.L. Therapeutic treatment of breast cancer based on c-MAF status
CN111330311B (zh) * 2020-02-13 2021-02-23 浙江大学 一种相变诱导靶标富集的方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5578444A (en) 1991-06-27 1996-11-26 Genelabs Technologies, Inc. Sequence-directed DNA-binding molecules compositions and methods
AU2636995A (en) * 1994-05-18 1995-12-05 Research And Development Institute, Inc. Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms
JPH10500310A (ja) 1994-05-19 1998-01-13 ダコ アクティーゼルスカブ 淋菌及びトラコーマ クラミジアの検出のためのｐｎａ プローブ
EP0891550A1 (en) 1996-04-05 1999-01-20 The Johns Hopkins University School Of Medicine A method of enriching rare cells
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
GB9716664D0 (en) 1997-08-06 1997-10-15 Norwegian Inst Of Fisheries & A method of removing nucleic acid contamination reactions
US20020172987A1 (en) * 1998-02-12 2002-11-21 Terstappen Leon W.M.M. Methods and reagents for the rapid and efficient isolation of circulating cancer cells
BR9907852A (pt) 1998-02-12 2000-10-24 Immunivest Corp Processos para detectar e enumerar células raras e cancerosas em uma população celular mista, para diagnosticar câncer de estágio precoce em um paciente de teste, para determinar a probabilidade de recorrência de câncer em um paciente humano anteriormente tratado de câncer, para distinguir um carcinoma confinado ao órgão de um carcinoma com propriedades metastáticas, para acompanhar a situação de remissão em um paciente humano com câncer que passa pelo tratamento de terapia contra o câncer e para aumentar quantidades de células epiteliais circulantes em uma amostra de sangue, partìcula magnética revestida, composição, conjuntos de teste para avaliar uma amostra de paciente quanto a presença de células raras circulantes, quanto a presença de células de tumor circulantes, quanto a presença de células de câncer de mama circulantes, quanto a presença de células de câncer de próstata circulantes, quanto a presença de células de câncer de cólon circulantes, quanto a presença de células de câncer de bexiga circulantes e para monitorar um paciente quanto a recorrência de câncer, e, fração de sangue periférico enriquecido quanto a células neoplásticas circulantes
EP1091973A4 (en) 1998-06-02 2005-03-23 Univ Yale IN-SITU HYBRIDIZATION IN MULTIPARAMETER FLUORESIS
US6429293B1 (en) 1998-06-26 2002-08-06 Hsc Research And Development Limited Partnership Sculpin-type antifreeze polypeptides and nucleic acids
US6960449B2 (en) 1999-02-10 2005-11-01 Cell Works Diagnostics, Inc. Class characterization of circulating cancer cells isolated from body fluids and methods of use
WO2001006014A1 (en) 1999-07-19 2001-01-25 American Laboratory Technologies, Inc. Removing repetitive sequences from dna
US6203992B1 (en) 1999-10-15 2001-03-20 Abbott Laboratories Nucleic acid primers and probes for detecting tumor cells
WO2001032930A1 (en) * 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
ATE359300T1 (de) 2001-01-19 2007-05-15 Ludwig Inst Cancer Res Flt4 (vegfr-3) als ein ziel für krebs darstellung und anti-krebs-behandlung
CA2708573C (en) 2001-02-12 2012-05-01 Richard L. Columbus Cartridge for containing a specimen sample for optical analysis
GB0111324D0 (en) * 2001-05-09 2001-07-04 Unilever Plc Ambient stable beverage
JP2005524384A (ja) 2001-09-24 2005-08-18 ボストン　プローブス，インコーポレイテッド ゲノム核酸におけるランダム分布反復配列への検出可能なプローブ結合の抑制に関する方法、キットおよび組成物
US20030082632A1 (en) 2001-10-25 2003-05-01 Cytoprint, Inc. Assay method and apparatus
AU2002360305A1 (en) 2001-10-26 2003-05-06 Immunivest Corporation Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample
AU2002346662A1 (en) 2001-12-04 2003-06-17 Euregen Llc Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture
ES2341948T3 (es) 2001-12-21 2010-06-30 Immunex Corporation Celulas madre endoteliales, poblaciones, metodos de aislamiento y uso de las mismas.
JP2005522224A (ja) 2002-04-12 2005-07-28 キュアレイターズ オブ ザ ユニバーシティー オブ ミズーリ Ｄｎａの高メチル化と遺伝子サイレンシングの両方をスクリーニングするためのｅｃｉｓｔマイクロアレイと、そのｅｃｉｓｔマイクロアレイを利用して遺伝子の発現、ｄｎａのメチル化、ヒストンのアセチル化を評価するための統合システム
AU2003234459A1 (en) 2002-05-03 2003-11-17 Immunivest Corporation Device and method for analytical cell imaging
US7648678B2 (en) 2002-12-20 2010-01-19 Dako Denmark A/S Method and system for pretreatment of tissue slides
ATE489452T1 (de) 2003-02-27 2010-12-15 Veridex Llc Zirkulierende tumorzellen (ctc's): frühe beurteilung von time-to-progression, überleben und ansprechen auf therapie bei patienten mit metastasiertem krebs
EP1604040B1 (en) 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a dna polymerization process
US20050064450A1 (en) 2003-03-13 2005-03-24 Lucas Joe N. Methods of making repetitive sequences removed probes and uses thereof
WO2004103389A2 (en) * 2003-05-22 2004-12-02 Theraptosis Sa Means for preventing and treating cellular death and their biological applications
WO2005028663A2 (en) 2003-09-18 2005-03-31 Immunivest Corporation Operator independent programmable sample preparation and analysis system
DE102005006237A1 (de) * 2005-02-10 2006-08-24 Henkel Kgaa Verfahren zur Bestimmung von Keimen
EP1722230A3 (en) 2005-04-27 2009-05-27 MPB MelTec Patent- und Beteiligungsgesellschaft mbH Method for detection of a disease-specific combinatorial molecular pattern motif in a tissue sample of a patient's skin
ES2886923T3 (es) 2005-09-20 2021-12-21 Menarini Silicon Biosystems Spa Métodos y composición para generar sondas de ADN de secuencia única, marcaje de sondas de ADN y el uso de estas sondas
US7659978B2 (en) * 2006-10-19 2010-02-09 Chemimage Corporation Photobleaching by high power pulses

Also Published As

Publication number Publication date
CN101672779B (zh) 2016-01-06
EP2169387A2 (en) 2010-03-31
KR20170094523A (ko) 2017-08-18
EP2169387A3 (en) 2011-03-09
BRPI0902559A2 (pt) 2011-04-05
EP2169387B1 (en) 2015-04-22
CN101672779A (zh) 2010-03-17
PL2169387T3 (pl) 2015-09-30
JP2010029187A (ja) 2010-02-12
CA2975186A1 (en) 2010-01-29
KR20100012847A (ko) 2010-02-08
DK2169387T3 (en) 2015-05-26
CA2674187C (en) 2017-09-12
BR122018015761B8 (pt) 2021-07-27
HK1140820A1 (en) 2010-10-22
JP6109464B2 (ja) 2017-04-05
PT2169387E (pt) 2015-06-03
US20090029378A1 (en) 2009-01-29
KR101764597B1 (ko) 2017-08-03
HUE026779T2 (en) 2016-07-28
BR122018015761B1 (pt) 2019-10-22
IL199798A0 (en) 2010-05-17
ES2542061T3 (es) 2015-07-30
CA2674187A1 (en) 2010-01-29
IL199798A (en) 2014-08-31
US9134237B2 (en) 2015-09-15

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