MX2009003736A - Metodo de deteccion de tamaños de acidos nucleicos. - Google Patents

Metodo de deteccion de tamaños de acidos nucleicos.

Info

Publication number
MX2009003736A
MX2009003736A MX2009003736A MX2009003736A MX2009003736A MX 2009003736 A MX2009003736 A MX 2009003736A MX 2009003736 A MX2009003736 A MX 2009003736A MX 2009003736 A MX2009003736 A MX 2009003736A MX 2009003736 A MX2009003736 A MX 2009003736A
Authority
MX
Mexico
Prior art keywords
nucleic acid
detection method
size detection
fragmentation
acid size
Prior art date
Application number
MX2009003736A
Other languages
English (en)
Inventor
Donghui Huang
Charles M Strom
Steven J Potts
Jenny Ellen Rooke
Original Assignee
Quest Diagnostics Invest Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quest Diagnostics Invest Inc filed Critical Quest Diagnostics Invest Inc
Publication of MX2009003736A publication Critical patent/MX2009003736A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

La presente invención proporciona métodos para determinar el tamaño de un segmento particular de ácido nucleico de interés en una muestra de ácidos nucleicos a través de fragmentación de ADN, fraccionamiento por tamaños, una segunda fragmentación opcional, e identificación usando una secuencia marcadora. En aspectos particulares, una expansión o reducción de secuencias de repeticiones en tándem puede detectarse. En aspectos posteriores, los portadores e individuos aquejados con síndrome X frágil u otras enfermedades asociadas con repeticiones en tándem pueden distinguirse de los individuos normales.
MX2009003736A 2006-10-05 2007-04-11 Metodo de deteccion de tamaños de acidos nucleicos. MX2009003736A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/544,293 US8163480B2 (en) 2006-10-05 2006-10-05 Nucleic acid size detection method
PCT/US2007/008985 WO2008045136A2 (en) 2006-10-05 2007-04-11 Nucleic acid size detection method

Publications (1)

Publication Number Publication Date
MX2009003736A true MX2009003736A (es) 2009-07-09

Family

ID=39283328

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009003736A MX2009003736A (es) 2006-10-05 2007-04-11 Metodo de deteccion de tamaños de acidos nucleicos.

Country Status (10)

Country Link
US (2) US8163480B2 (es)
EP (1) EP2082055B1 (es)
JP (1) JP5386357B2 (es)
CN (1) CN101595227A (es)
AT (1) ATE515573T1 (es)
AU (1) AU2007307320B2 (es)
BR (1) BRPI0720549A2 (es)
CA (1) CA2665723A1 (es)
MX (1) MX2009003736A (es)
WO (1) WO2008045136A2 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8163480B2 (en) * 2006-10-05 2012-04-24 Quest Diagnostics Investments Incorporated Nucleic acid size detection method
US20080176237A1 (en) * 2006-12-07 2008-07-24 Biocept, Inc. Non-invasive prenatal genetic screen
US8409805B2 (en) * 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
WO2010094061A1 (en) * 2009-02-17 2010-08-26 Murdoch Childrens Research Institute Assay for determining epigenetic profiles of markers of fragile x alleles
CN102449171B (zh) * 2009-03-24 2015-12-02 奥斯瑞根公司 对fmr1和fmr2基因5’非翻译区进行表征的pcr方法
CN101871001B (zh) * 2009-04-22 2013-09-25 中山大学达安基因股份有限公司 检测脆性x综合征的试剂盒
ES2573092T3 (es) * 2009-07-10 2016-06-06 Perkinelmer Health Sciences, Inc. Detección de repeticiones de multinucleótido
US9382586B2 (en) 2010-02-05 2016-07-05 Quest Diagnostics Investments Incorporated Method to detect repeat sequence motifs in nucleic acid
AU2011288917B2 (en) 2010-08-11 2015-01-22 Murdoch Childrens Research Institute Treatment and diagnosis of epigenetic disorders and conditions
WO2012031021A2 (en) * 2010-09-01 2012-03-08 Orion Genomics Llc Improved detection of nucleic acid sequences adjacent to repeated sequences
US9476043B2 (en) * 2011-04-08 2016-10-25 Rula Zain-Luqman Diagnosis and treatment of friedreich's ataxia
AU2012318290B2 (en) 2011-11-04 2015-07-30 Gen-Probe Incorporated Molecular assay reagents and methods
WO2014015273A1 (en) 2012-07-20 2014-01-23 Asuragen, Inc. Comprehensive fmr1 genotyping
EP2732815A1 (en) * 2012-11-16 2014-05-21 Neurochlore Modulators of intracellular chloride concentration for treating fragile X syndrome
GB201301164D0 (en) * 2013-01-23 2013-03-06 Medical Res Council Methods for estimating the size of disease-associated polynucleotide repaet expansions in genes
CA3174919A1 (en) * 2013-03-14 2014-09-25 Quest Diagnostics Investments Incorporated Method for detecting cystic fibrosis
US9273349B2 (en) * 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
GB201418144D0 (en) * 2014-10-14 2014-11-26 Univ Cardiff High throughput sequencing
CN104816447B (zh) * 2015-04-30 2017-03-08 青岛科技大学 一种行星齿轮式混炼装置
CN104862302B (zh) * 2015-05-05 2020-12-15 华南师范大学 一种dna片段化的方法及实现该方法的装置
GB201511508D0 (en) 2015-07-01 2015-08-12 Ge Healthcare Bio Sciences Ab Method for determining a size of biomolecules
JP2019013206A (ja) * 2017-07-11 2019-01-31 国立大学法人帯広畜産大学 アゾール耐性アスペルギルス・フミガーツスの検出方法および検出キット
CN110184344A (zh) * 2019-06-28 2019-08-30 北京和合医学诊断技术股份有限公司 检测htt基因cag三核苷酸重复序列的方法及引物对
CN110923305B (zh) * 2019-11-25 2023-12-29 广州市达瑞生物技术股份有限公司 一种适用于脆性X综合征southern blot印迹杂交检测的DNA分子量标准
US11319613B2 (en) 2020-08-18 2022-05-03 Enviro Metals, LLC Metal refinement
CN115851915A (zh) * 2022-12-13 2023-03-28 长沙金域医学检验实验室有限公司 检测遗传性共济失调致病基因的引物组及方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8606719D0 (en) * 1986-03-19 1986-04-23 Lister Preventive Med Genetic probes
US5135717A (en) * 1986-12-24 1992-08-04 British Technology Group Usa Inc. Tetrabenztriazaporphyrin reagents and kits containing the same
US5206137A (en) * 1988-09-08 1993-04-27 Lifecodes Corporation Compositions and methods useful for genetic analysis
US5714327A (en) * 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
NL9001639A (nl) * 1990-07-19 1992-02-17 Amc Amsterdam Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen.
US5364759B2 (en) * 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
US5652099A (en) * 1992-02-12 1997-07-29 Conrad; Michael J. Probes comprising fluorescent nucleosides and uses thereof
US5695933A (en) * 1993-05-28 1997-12-09 Massachusetts Institute Of Technology Direct detection of expanded nucleotide repeats in the human genome
US5599666A (en) * 1994-03-28 1997-02-04 Promega Corporation Allelic ladders for short tandem repeat loci
US6013444A (en) * 1997-09-18 2000-01-11 Oligotrail, Llc DNA bracketing locus compatible standards for electrophoresis
US6238863B1 (en) * 1998-02-04 2001-05-29 Promega Corporation Materials and methods for indentifying and analyzing intermediate tandem repeat DNA markers
US6074831A (en) * 1998-07-09 2000-06-13 Agilent Technologies, Inc. Partitioning of polymorphic DNAs
EP1384022A4 (en) * 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US7855053B2 (en) 2006-07-19 2010-12-21 The Regents Of The University Of California Methods for detecting the presence of expanded CGG repeats in the FMR1 gene 5′ untranslated region
US8163480B2 (en) * 2006-10-05 2012-04-24 Quest Diagnostics Investments Incorporated Nucleic acid size detection method

Also Published As

Publication number Publication date
US20080124709A1 (en) 2008-05-29
US8163480B2 (en) 2012-04-24
EP2082055A4 (en) 2010-02-17
CN101595227A (zh) 2009-12-02
JP2010505413A (ja) 2010-02-25
CA2665723A1 (en) 2008-04-17
US20100167284A1 (en) 2010-07-01
JP5386357B2 (ja) 2014-01-15
EP2082055A2 (en) 2009-07-29
EP2082055B1 (en) 2011-07-06
WO2008045136A2 (en) 2008-04-17
WO2008045136A3 (en) 2008-12-11
AU2007307320B2 (en) 2013-07-11
ATE515573T1 (de) 2011-07-15
AU2007307320A1 (en) 2008-04-17
US8697399B2 (en) 2014-04-15
BRPI0720549A2 (pt) 2014-07-29

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