MX2007009547A - Metodo de deteccion de fiebre porcina clasica. - Google Patents

Metodo de deteccion de fiebre porcina clasica.

Info

Publication number
MX2007009547A
MX2007009547A MX2007009547A MX2007009547A MX2007009547A MX 2007009547 A MX2007009547 A MX 2007009547A MX 2007009547 A MX2007009547 A MX 2007009547A MX 2007009547 A MX2007009547 A MX 2007009547A MX 2007009547 A MX2007009547 A MX 2007009547A
Authority
MX
Mexico
Prior art keywords
csfv
assay
detection
swine fever
classical swine
Prior art date
Application number
MX2007009547A
Other languages
English (en)
Inventor
Bernd Hoffmann
Klaus Depner
Martin Beer
Original Assignee
Idexx Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idexx Lab Inc filed Critical Idexx Lab Inc
Publication of MX2007009547A publication Critical patent/MX2007009547A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invencion se refiere a un ensayo RT-PCR en tiempo real, multiplex con un sistema de control interno heterologo (es decir, EGFP-RNA) para el diagnostico sencillo y rapido del virus de fiebre porcina clasica (CSFV). Los cebadores y las sondas TaqMan etiquetadas con FAM, especificas para CSFV fueron seleccionados analizando la secuencia consenso de la region 5??-no traducida de diferentes cepas de CSFV. Para determinar la sensibilidad analitica se construyo y analizo un transcrito in vitro (T7-PC3Alf) de la 5-NTR. Ademas, se establecio un sistema cebador-sonda para la deteccion de la secuencia de control interno y un ensayo multiplex utilizando el sistema CSF 1 y la PCR en tiempo real IC podria realizarse como un ensayo en un tubo sin perdida de sensibilidad ni especificidad.
MX2007009547A 2005-02-09 2006-02-08 Metodo de deteccion de fiebre porcina clasica. MX2007009547A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/052,762 US7608433B2 (en) 2005-02-09 2005-02-09 Method of detection of classical swine fever
PCT/US2006/004271 WO2006086377A2 (en) 2005-02-09 2006-02-08 Method of detection of classical swine fever

Publications (1)

Publication Number Publication Date
MX2007009547A true MX2007009547A (es) 2008-03-14

Family

ID=36780400

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2007009547A MX2007009547A (es) 2005-02-09 2006-02-08 Metodo de deteccion de fiebre porcina clasica.

Country Status (7)

Country Link
US (1) US7608433B2 (es)
EP (1) EP1856297B1 (es)
JP (1) JP2008529527A (es)
KR (1) KR101394421B1 (es)
CA (1) CA2597383C (es)
MX (1) MX2007009547A (es)
WO (1) WO2006086377A2 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200700557A (en) * 2005-06-17 2007-01-01 Animal Health Res Inst Council Of Agriculture Rt-pcr detection for differential diagnosis field isolates or lapinized vaccine strain of classical swine fever virus (csfv) in samples
CN101328506B (zh) * 2008-07-24 2010-11-03 湖北省农业科学院畜牧兽医研究所 一种特异检测猪瘟病毒野毒感染的荧光定量pcr快速诊断试剂盒及其应用方法
JP5589543B2 (ja) * 2010-05-10 2014-09-17 富士ゼロックス株式会社 映像信号伝送装置及び信号伝送プログラム
EP2392680A1 (en) * 2010-06-04 2011-12-07 Labor Diagnostik GmbH Leipzig Method for specific detection of classical swine fever virus
CN102212623B (zh) * 2011-06-02 2013-01-16 广东省农业科学院兽医研究所 一种猪瘟病毒和猪蓝耳病病毒的双色荧光定量pcr联合检测方法及其试剂盒
GB201204776D0 (en) * 2012-03-19 2012-05-02 Vela Operations Pte Ltd Positive control for PCR
CN103436633A (zh) * 2013-06-29 2013-12-11 中国农业科学院兰州兽医研究所 一种用于检测猪瘟病毒的Taqman Real-time RT-PCR试剂盒
CN105506185A (zh) * 2016-02-17 2016-04-20 南阳师范学院 一种猪瘟病毒的检测引物和荧光定量rt-pcr检测方法
CN111593139B (zh) * 2020-05-13 2022-08-02 中国农业大学 检测猪瘟病毒的rt-raa引物、探针和试剂盒及应用
CN111471803A (zh) * 2020-05-29 2020-07-31 武汉生命之美科技有限公司 一种新型冠状病毒covid-19感染检测试剂盒
CN111876475A (zh) * 2020-08-10 2020-11-03 湖南冠牧生物科技有限公司 一种内标组合物及其在检测动物病原微生物中的应用
CN113999939A (zh) * 2021-12-30 2022-02-01 北京纳百生物科技有限公司 一种用于猪瘟病毒荧光定量rt-pcr检测的引物及试剂盒

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DE3329892A1 (de) 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4786724A (en) 1984-10-11 1988-11-22 Northwestern University Nucleoside trichlorodimethylethyl phosphite esters
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
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FR2596761B1 (fr) 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
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Also Published As

Publication number Publication date
JP2008529527A (ja) 2008-08-07
EP1856297A2 (en) 2007-11-21
WO2006086377A2 (en) 2006-08-17
CA2597383A1 (en) 2006-08-17
CA2597383C (en) 2015-05-26
EP1856297A4 (en) 2010-02-03
WO2006086377A3 (en) 2009-05-22
KR101394421B1 (ko) 2014-05-14
US7608433B2 (en) 2009-10-27
US20060177818A1 (en) 2006-08-10
KR20070112789A (ko) 2007-11-27
EP1856297B1 (en) 2014-11-26

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