MD705Y - Procedeu de multiplicare microclonala a vitei-de-vie - Google Patents
Procedeu de multiplicare microclonala a vitei-de-vie Download PDFInfo
- Publication number
- MD705Y MD705Y MDS20130071A MDS20130071A MD705Y MD 705 Y MD705 Y MD 705Y MD S20130071 A MDS20130071 A MD S20130071A MD S20130071 A MDS20130071 A MD S20130071A MD 705 Y MD705 Y MD 705Y
- Authority
- MD
- Moldova
- Prior art keywords
- regenerants
- murashige
- transferred
- skoog
- grapevine
- Prior art date
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Inventia se refera la biotehnologiile agricole si poate fi utilizata pentru multiplicarea microclonala a vitei-de-vie.Procedeul, conform inventiei, consta în aceea ca se selecteaza lastari cu lungimea de 6…10 cm, se spala, se clatesc în apa curgatoare, se sterilizeaza, se excizeaza apexuri cu marimea de 0,5…1,0 mm, ce contin meristeme cu 2…3 frunzulite primordiale, care se inoculeaza în mediul nutritiv Murashige-Skoog suplimentat cu 5 µM de 6-benzilaminopurina, 0,5 µM de acid naftalinacetic, 2% de zaharoza, 0,7% de agar, având pH-ul 5,7; dupa 2…3 saptamâni de la inoculare apexurile se iradiaza în regim periodic, timp de 20…25 min, cu unde milimetrice de intensitate joasa cu lungimea de unda de 5,6 mm si frecventa de 53,8 GHz, dupa ce regenerantii prolifereaza si se dezvolta ei se transfera pe mediul Murashige-Skoog, se subcultiva pe parcursul a 6…7 cicluri si se efectueaza microbutasirea lor in vitro, regenerantii cu 3…4 internoduri se transfera pe mediul Murashige-Skoog suplimentat cu 0,4 µM de acid naftalinacetic, apoi regenerantii cu sistem radicular bine dezvoltat se transfera în amestec de sol si turba, iar ulterior pe substrat de sol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130071A MD705Z (ro) | 2013-04-19 | 2013-04-19 | Procedeu de multiplicare microclonală a viţei-de-vie |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20130071A MD705Z (ro) | 2013-04-19 | 2013-04-19 | Procedeu de multiplicare microclonală a viţei-de-vie |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD705Y true MD705Y (ro) | 2013-12-31 |
| MD705Z MD705Z (ro) | 2014-07-31 |
Family
ID=49912588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20130071A MD705Z (ro) | 2013-04-19 | 2013-04-19 | Procedeu de multiplicare microclonală a viţei-de-vie |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD705Z (ro) |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2041609C1 (ru) * | 1993-02-01 | 1995-08-20 | Научно-Производственное Объединение "Виноград" | Способ микроклонального размножения винограда "ин витро" |
| RU2077192C1 (ru) * | 1995-03-29 | 1997-04-20 | Научно-Производственное Объединение "Виноград" | Способ размножения винограда in vitro |
| MD701G2 (ro) * | 1996-09-20 | 1998-02-28 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de cultivare a viţei de vie |
| MD1089G2 (ro) * | 1997-05-07 | 1999-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Procedeu de cultivare a viţei de vie |
| MD1603G2 (ro) * | 1999-12-20 | 2001-08-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de cultivare a viţei de vie |
| RU2265319C2 (ru) * | 2003-09-11 | 2005-12-10 | ГНУ Всероссийский НИИ виноградарства и виноделия им. Я.И. Потапенко (ВНИИВиВ) | Способ регенерации меристем |
| RU2264706C2 (ru) * | 2003-09-11 | 2005-11-27 | ГНУ Всероссийский НИИ виноградарства и виноделия им. Я.И. Потапенко (ВНИИВиВ) | Способ оптимизации клонального микроразмножения винограда in vitro |
| MD2924G2 (ro) * | 2005-04-06 | 2006-07-31 | Институт Физиологии Растений Академии Наук Республики Молдова | Procedeu de tratare a viţei de vie |
| RU2332838C1 (ru) * | 2007-01-31 | 2008-09-10 | Государственное общеобразовательное учреждение высшего профессионального образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Способ вегетативного размножения черенков винограда |
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2013
- 2013-04-19 MD MDS20130071A patent/MD705Z/ro not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD705Z (ro) | 2014-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |