MD705Y - Process for microclonal propagation of grapevine - Google Patents

Process for microclonal propagation of grapevine Download PDF

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Publication number
MD705Y
MD705Y MDS20130071A MDS20130071A MD705Y MD 705 Y MD705 Y MD 705Y MD S20130071 A MDS20130071 A MD S20130071A MD S20130071 A MDS20130071 A MD S20130071A MD 705 Y MD705 Y MD 705Y
Authority
MD
Moldova
Prior art keywords
regenerants
murashige
transferred
skoog
grapevine
Prior art date
Application number
MDS20130071A
Other languages
Moldavian (mo)
Romanian (ro)
Inventor
Svetlana Smerea
Larisa Andronic
Anatol ROTARU
Original Assignee
Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei filed Critical Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei
Priority to MDS20130071A priority Critical patent/MD705Z/en
Publication of MD705Y publication Critical patent/MD705Y/en
Publication of MD705Z publication Critical patent/MD705Z/en

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14—Measures for saving energy, e.g. in green houses

Landscapes

  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to agricultural biotechnology and can be used for microclonal propagation of grapevine.The process, according to the invention, consists in that shoots of a length of 6…10 cm are selected, washed, rinsed in running water, sterilized, apexes of a size of 0.5…1.0 mm are excised, containing meristems with 2…3 primordial leaves, which are inoculated in the Murashige-Skoog nutrient medium supplemented with 5 µM of 6-benzylaminopurine, 0.5 µM of naphthalene-acetic acid, 2% of sucrose, 0.7% of agar, having the pH 5.7; in 2…3 weeks after inoculation apexes are irradiated in periodic regime, during 20…25 min, with low-intensity millimetric waves of a wavelength of 5.6 mm and a frequency of 53.8 GHz, after the regenerants proliferate and develop they are transferred to the Murashige-Skoog medium, are subcultured during 6…7 cycles and is performed their micrografting in vitro, regenerants with 3…4 interstices are transferred to the Murashige-Skoog medium supplemented with 0.4 µM of naphthalene-acetic acid, then the regenerants with well-developed radicular system are transferred into a mixture of soil and peat, and then into soil substrate.
MDS20130071A 2013-04-19 2013-04-19 Process for microclonal propagation of grapevine MD705Z (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDS20130071A MD705Z (en) 2013-04-19 2013-04-19 Process for microclonal propagation of grapevine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDS20130071A MD705Z (en) 2013-04-19 2013-04-19 Process for microclonal propagation of grapevine

Publications (2)

Publication Number Publication Date
MD705Y true MD705Y (en) 2013-12-31
MD705Z MD705Z (en) 2014-07-31

Family

ID=49912588

Family Applications (1)

Application Number Title Priority Date Filing Date
MDS20130071A MD705Z (en) 2013-04-19 2013-04-19 Process for microclonal propagation of grapevine

Country Status (1)

Country Link
MD (1) MD705Z (en)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2041609C1 (en) * 1993-02-01 1995-08-20 Научно-Производственное Объединение "Виноград" Method of microclonal reproduction of grape
RU2077192C1 (en) * 1995-03-29 1997-04-20 Научно-Производственное Объединение "Виноград" Method for in vitro propagation of grape
MD701G2 (en) * 1996-09-20 1998-02-28 Институт Физиологии Растений Академии Наук Республики Молдова Process for cultivation of the grape-vine
MD1089G2 (en) * 1997-05-07 1999-05-31 Институт Генетики И Физиологии Растений Академии Наук Молдовы Vine cultivation process
MD1603G2 (en) * 1999-12-20 2001-08-31 Институт Физиологии Растений Академии Наук Республики Молдова Process for vine-growing
RU2264706C2 (en) * 2003-09-11 2005-11-27 ГНУ Всероссийский НИИ виноградарства и виноделия им. Я.И. Потапенко (ВНИИВиВ) Method for optimization of clonal in vitro micro multiplication of grape
RU2265319C2 (en) * 2003-09-11 2005-12-10 ГНУ Всероссийский НИИ виноградарства и виноделия им. Я.И. Потапенко (ВНИИВиВ) Meristem regeneration method
MD2924G2 (en) * 2005-04-06 2006-07-31 Институт Физиологии Растений Академии Наук Республики Молдова process for vine treatment
RU2332838C1 (en) * 2007-01-31 2008-09-10 Государственное общеобразовательное учреждение высшего профессионального образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) Method of reproduction of grafts of grapes
  • 2013

Also Published As

Publication number Publication date
MD705Z (en) 2014-07-31

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Legal Events

Date Code Title Description
FG9Y Short term patent issued
KA4Y Short-term patent lapsed due to non-payment of fees (with right of restoration)