MD4474C1 - Защитная среда для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 - Google Patents
Защитная среда для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 Download PDFInfo
- Publication number
- MD4474C1 MD4474C1 MDA20160086A MD20160086A MD4474C1 MD 4474 C1 MD4474 C1 MD 4474C1 MD A20160086 A MDA20160086 A MD A20160086A MD 20160086 A MD20160086 A MD 20160086A MD 4474 C1 MD4474 C1 MD 4474C1
- Authority
- MD
- Moldova
- Prior art keywords
- cnmn
- lyophilization
- strain
- canosus
- streptomyces
- Prior art date
Links
- 238000004108 freeze drying Methods 0.000 title claims abstract description 24
- 230000001681 protective effect Effects 0.000 title claims abstract description 13
- 241000187747 Streptomyces Species 0.000 title claims abstract description 11
- 230000007774 longterm Effects 0.000 title claims abstract description 11
- 150000004676 glycans Chemical class 0.000 claims abstract description 13
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 13
- 239000005017 polysaccharide Substances 0.000 claims abstract description 13
- 108010010803 Gelatin Proteins 0.000 claims abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 239000008273 gelatin Substances 0.000 claims abstract description 9
- 229920000159 gelatin Polymers 0.000 claims abstract description 9
- 235000019322 gelatine Nutrition 0.000 claims abstract description 9
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 5
- 239000002028 Biomass Substances 0.000 claims abstract description 5
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 4
- 241001464430 Cyanobacterium Species 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 239000012047 saturated solution Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- WCIILWFNDMVNFM-UHFFFAOYSA-M [Br-].C(CCCCCCCCCCCCCCC)[N+](C)(C)C.[Na] Chemical compound [Br-].C(CCCCCCCCCCCCCCC)[N+](C)(C)C.[Na] WCIILWFNDMVNFM-UHFFFAOYSA-M 0.000 claims 1
- 235000019441 ethanol Nutrition 0.000 claims 1
- 238000004062 sedimentation Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000002609 medium Substances 0.000 description 16
- 230000035899 viability Effects 0.000 description 13
- 239000000203 mixture Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 2
- 239000012225 czapek media Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- -1 Cyclopropane Fatty Acids Chemical class 0.000 description 1
- 108700005443 Microbial Genes Proteins 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 241000320117 Pseudomonas putida KT2440 Species 0.000 description 1
- 241000192500 Spirulina sp. Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 241001446247 uncultured actinomycete Species 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к защитной среде для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02.Среда, согласно изобретению, содержит 7,5% глюкозы, 2,5% желатина и 1,0…50,0%об. экстракта сульфатированных полисахаридов с концентрацией 10 мг/мл, полученного из биомассы штамма цианобактерии Spirulina platensis CNMN-CB-02.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20160086A MD4474C1 (ru) | 2016-07-15 | 2016-07-15 | Защитная среда для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20160086A MD4474C1 (ru) | 2016-07-15 | 2016-07-15 | Защитная среда для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4474B1 MD4474B1 (ru) | 2017-03-31 |
| MD4474C1 true MD4474C1 (ru) | 2017-10-31 |
Family
ID=58412684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20160086A MD4474C1 (ru) | 2016-07-15 | 2016-07-15 | Защитная среда для лиофилизации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4474C1 (ru) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1235Z (ru) * | 2017-06-29 | 2018-09-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Защитная среда для консервации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 |
| MD1226Z (ru) * | 2017-06-29 | 2018-08-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Защитная среда для консервации и длительного хранения штамма Streptomyces canosus CNMN-Ac-02 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05308956A (ja) * | 1991-03-19 | 1993-11-22 | Mitsubishi Heavy Ind Ltd | 硝酸菌の保存と復元方法 |
| JPH05308955A (ja) * | 1991-03-19 | 1993-11-22 | Mitsubishi Heavy Ind Ltd | 亜硝酸菌の保存と復元方法 |
| MD617B1 (en) * | 1995-08-28 | 1996-11-29 | Univ Nicolae Testemitanu | The nutritiv medium for cultivation of campylobacteriums |
| MD1353F1 (en) * | 1998-12-03 | 1999-11-30 | Inst De Microbiologie Al Acade | Nutrient medium for cultivation of streptomycetes |
| MD20010233A (en) * | 2001-07-24 | 2003-02-28 | Inst De Microbiologie Al Acade | Nutrient medium for cultivation of fluorescent pseudomonads |
| UA27038U (en) * | 2007-06-19 | 2007-10-10 | Inst Problems Of Cryobiology & Cryomedicine | A method for conservation of spore culture of streptomyces aureofaciens |
| RU2006138233A (ru) * | 2004-03-31 | 2008-05-10 | Даниско А/С (Dk) | Способ |
-
2016
- 2016-07-15 MD MDA20160086A patent/MD4474C1/ru not_active IP Right Cessation
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05308956A (ja) * | 1991-03-19 | 1993-11-22 | Mitsubishi Heavy Ind Ltd | 硝酸菌の保存と復元方法 |
| JPH05308955A (ja) * | 1991-03-19 | 1993-11-22 | Mitsubishi Heavy Ind Ltd | 亜硝酸菌の保存と復元方法 |
| MD617B1 (en) * | 1995-08-28 | 1996-11-29 | Univ Nicolae Testemitanu | The nutritiv medium for cultivation of campylobacteriums |
| MD1353F1 (en) * | 1998-12-03 | 1999-11-30 | Inst De Microbiologie Al Acade | Nutrient medium for cultivation of streptomycetes |
| MD20010233A (en) * | 2001-07-24 | 2003-02-28 | Inst De Microbiologie Al Acade | Nutrient medium for cultivation of fluorescent pseudomonads |
| RU2006138233A (ru) * | 2004-03-31 | 2008-05-10 | Даниско А/С (Dk) | Способ |
| UA27038U (en) * | 2007-06-19 | 2007-10-10 | Inst Problems Of Cryobiology & Cryomedicine | A method for conservation of spore culture of streptomyces aureofaciens |
Non-Patent Citations (4)
| Title |
|---|
| Chiseliţa Oleg, Burţeva Svetlana, Bîrsa Maxim, Bulimaga Valentina, Vasiliciuc Anastasia. Viability and antimicrobial activity of streptomyces strains from NCNM after lyophilization. Studia Universitatis Moldaviae, Seria Stiinţe reale şi ale naturii, 2016, nr.1(91), p. 61-71 * |
| Yocheva L., Najdenova M., Doncheva D., Antonova-Niconova S. Influence of the long-term preservation on some biological features of three streptomycetes strains, producers of antibiotic substances. Journal of culture collection, 2002, v. 3, p. 25-32 * |
| Куплетская М. Б., Аркадьева З.А. Методы длительного хранения коллекции микроорганизмов кафедры микробиологии МГУ. Микробиология, 1997, т. 66, № 2, р. 283-288 * |
| Нечисляев В. А., Орлова В. Е., Бахтин И. А. Универсальная защитня среда для лиофилизации пробиотических преператов. Казанский медицинский журнал. 2010, том 91, №1. Găsit internet: <URL:http://cyberleninka.ru/article/n/universalnaya-zaschitnaya-sreda-dlya-liofilizatsii-probioticheskih-preparatov> * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4474B1 (ru) | 2017-03-31 |
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|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |