MD3099G2 - Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex - Google Patents
Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complexInfo
- Publication number
- MD3099G2 MD3099G2 MDA20050349A MD20050349A MD3099G2 MD 3099 G2 MD3099 G2 MD 3099G2 MD A20050349 A MDA20050349 A MD A20050349A MD 20050349 A MD20050349 A MD 20050349A MD 3099 G2 MD3099 G2 MD 3099G2
- Authority
- MD
- Moldova
- Prior art keywords
- hyaluronic acid
- ratio
- sediment
- stage
- protein
- Prior art date
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex that may be used in the food, pharmaceutical and cosmetic industries.Summary of the invention consists in that the process includes washing of the hen, cock combs with water at the temperature of 10…15°C during 5…6 hours, comminution and dehydration thereof in acetone or 96% ethanol containing 1% CHCl3, in the ratio of 1:3, afterwards they are left for 6…24 hours at the temperature of 0…4°C, then the solvent is separated and it is carried out the additional dehydration and acetone degreasing by boiling during 2 hours, the obtained sediment is dried and subjected to extraction with aqueous solution of 1 M NaCl in two stages: the I-st stage in cold at 4…10°C and the II-nd stage at heating up to 50…60°C, at the same time in the I-st stage it is carried out the thrice extraction in the ratio of 1:20, it is separated the sediment from the extract, then it is carried out the hyaluronic acid sedimentation from the extract with 96% ethanol in the ratio of 1:3, the sediment is redissolved and there are removed the proteins out of it by heating and cooling at pH 5,0…5,5, it is added CHCl3 in the ratio of 1:1, it is separated the aqueous phase, it is treated with 96% ethanol in the ratio of 1:3, it is separated the formed sodium hyaluronate sediment with a protein content of at most 1%, and the hyaluronic acid is obtained by acidulation with HCl of the aqueous sodium hyaluronate solution; in the II-nd stage the sediment, obtained in the I-st stage, is extracted in the ratio of 1:3, then it is settled with acetone, obtaining a protein-hyaluronic acid complex with a protein content of 65%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20050349A MD3099G2 (en) | 2005-11-29 | 2005-11-29 | Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20050349A MD3099G2 (en) | 2005-11-29 | 2005-11-29 | Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD3099F1 MD3099F1 (en) | 2006-07-31 |
| MD3099G2 true MD3099G2 (en) | 2007-02-28 |
Family
ID=36808911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20050349A MD3099G2 (en) | 2005-11-29 | 2005-11-29 | Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD3099G2 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4141973A (en) * | 1975-10-17 | 1979-02-27 | Biotrics, Inc. | Ultrapure hyaluronic acid and the use thereof |
| RU2055079C1 (en) * | 1992-02-04 | 1996-02-27 | Научно-производственное акционерное общество "Техкон" | Method for production of preparation of hyaluronic acid |
| RU2074196C1 (en) * | 1993-08-23 | 1997-02-27 | Научно-производственная фирма "Нарт" | Method of hyaluronic acid preparing |
| RU2115662C1 (en) * | 1997-07-21 | 1998-07-20 | Самойленко Игорь Иннокентьевич | Method of preparing hyaluronic acid |
| MD1617G2 (en) * | 2000-03-28 | 2001-08-31 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Process for preparation of the hyaluronic acid |
-
2005
- 2005-11-29 MD MDA20050349A patent/MD3099G2/en not_active IP Right Cessation
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4141973A (en) * | 1975-10-17 | 1979-02-27 | Biotrics, Inc. | Ultrapure hyaluronic acid and the use thereof |
| US4141973B1 (en) * | 1975-10-17 | 1989-08-08 | ||
| RU2055079C1 (en) * | 1992-02-04 | 1996-02-27 | Научно-производственное акционерное общество "Техкон" | Method for production of preparation of hyaluronic acid |
| RU2074196C1 (en) * | 1993-08-23 | 1997-02-27 | Научно-производственная фирма "Нарт" | Method of hyaluronic acid preparing |
| RU2115662C1 (en) * | 1997-07-21 | 1998-07-20 | Самойленко Игорь Иннокентьевич | Method of preparing hyaluronic acid |
| MD1617G2 (en) * | 2000-03-28 | 2001-08-31 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Process for preparation of the hyaluronic acid |
Also Published As
| Publication number | Publication date |
|---|---|
| MD3099F1 (en) | 2006-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |