MD3099F1

MD3099F1 - Process for obtaining the sodium hyaluronate, the hyaluronic acid and the protein-hyaluronic acid complex

Info

Publication number: MD3099F1
Application number: MDA20050349A
Authority: MD
Inventors: Larisa Zadorojnai
Original assignee: Universitatea Tehnica A Moldovei
Priority date: 2005-11-29
Filing date: 2005-11-29
Publication date: 2006-07-31
Also published as: MD3099G2

Abstract

Inventia se refera la un procedeu de obtinere a hialuronatului de sodiu, acidului hialuronic si complexului acid hialuronic-proteina, care pot fi utilizate in industria alimentara, farmaceutica si cosmetica. Esenta inventiei consta in aceea ca procedeul include spalarea crestelor de cocosi, gaini cu apa la temperatura de 10...15°C timp de 5...6 ore, maruntirea si deshidratarea lor in acetona sau etanol de 96%, care contine 1% CHCl3, in raport de1:3, dupa care se lasa pentru 6...24 ore la temperatura de 0...4°C, apoi solventul se separa si se efectueaza deshidratarea suplimentara si degresarea cu acetona la fierbere timp de 2 ore, sedimentulobtinut se usuca si se supune extragerii cu solutie apoasa de 1M NaCl in doua stadii: I stadiu la rece la 4...10°C si al II-lea stadiu la incalzire pana la 50...60°C, totodata la I stadiu se efectueaza extragerea in raport de 1:20 de trei ori, se separa reziduul de extract, dupa care se efectueaza sedimentarea acidului hialuronic din extract cu etanol de 96% in raport de 1:3, sedimentul se redizolva si se elimina proteinele din el la incalzire si racire la pH 5,0...5,5, se adauga CHCl3 in raport de 1:1, se separa faza apoasa, se trateaza cu etanol de 96% in raport de 1:3, se separa sedimentulformat de hialuronat de sodiu cu un continut de proteina de cel mult 1%, iar acidul hialuronic se obtine la acidularea cu HCl a solutiei apoase de hialuronat de sodiu; la al II-lea stadiu reziduul obtinut la I stadiu se extrage in raport de 1:3, apoi se sedimenteaza cu acetona obtinand un complex acid hialuronic-proteina cu un continut de proteinade 65%.The invention relates to a process for obtaining sodium hyaluronate, hyaluronic acid and hyaluronic acid-protein complex, which can be used in the food, pharmaceutical and cosmetic industries. The essence of the invention is that the process includes washing coconut ridges, chickens with water at 10 ... 15 ° C for 5 ... 6 hours, grinding and dehydrating them in acetone or 96% ethanol, which contains 1 % CHCl3, in a ratio of 1: 3, then left for 6 ... 24 hours at 0 ... 4 ° C, then the solvent was separated and further dehydration and boiling with acetone were boiled for 2 hours. , the obtained sediment is dried and subjected to extraction with 1M NaCl aqueous solution in two stages: I cold stage at 4 ... 10 ° C and the second stage when heated up to 50 ... 60 ° C, at the same time Stage I extraction is performed in a ratio of 1:20 three times, the residue is separated from the extract, after which the sedimentation of the hyaluronic acid from the extract with 96% ethanol in a ratio of 1: 3 is performed, the sediment is redissolved and the proteins are removed from on heating and cooling to pH 5.0 ... 5.5, add CHCl3 in a ratio of 1: 1, separate the aqueous phase, treat with ethanol of 96% with a ratio of 1: 3, the sediment formed with sodium hyaluronate is separated with a protein content of not more than 1%, and the hyaluronic acid is obtained by acidifying the aqueous sodium hyaluronate with HCl; at the second stage the residue obtained at the first stage is extracted in a ratio of 1: 3, then sedimented with acetone to obtain a hyaluronic acid-protein complex with a protein content of 65%.