MD1624Z - Способ глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз - Google Patents
Способ глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз Download PDFInfo
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- MD1624Z MD1624Z MDS20210053A MDS20210053A MD1624Z MD 1624 Z MD1624 Z MD 1624Z MD S20210053 A MDS20210053 A MD S20210053A MD S20210053 A MDS20210053 A MD S20210053A MD 1624 Z MD1624 Z MD 1624Z
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- Moldova
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- cultivation
- strain
- cnmn
- lipases
- rhizopus arrhizus
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- 239000004367 Lipase Substances 0.000 title claims abstract description 23
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 6
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- SNQQJEJPJMXYTR-UHFFFAOYSA-N dimethyl pyridine-2,6-dicarboxylate Chemical compound COC(=O)C1=CC=CC(C(=O)OC)=N1 SNQQJEJPJMXYTR-UHFFFAOYSA-N 0.000 claims description 3
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- 230000007613 environmental effect Effects 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 101100396546 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) tif-6 gene Proteins 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- KJNGJIPPQOFCSK-UHFFFAOYSA-N [H][Sr][H] Chemical compound [H][Sr][H] KJNGJIPPQOFCSK-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 1
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- 230000036983 biotransformation Effects 0.000 description 1
- 150000001869 cobalt compounds Chemical class 0.000 description 1
- FDBAIUSFRPWFTD-UHFFFAOYSA-L cobalt(2+) dithiocyanate trihydrate Chemical compound O.O.O.[Co++].[S-]C#N.[S-]C#N FDBAIUSFRPWFTD-UHFFFAOYSA-L 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
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- 238000012786 cultivation procedure Methods 0.000 description 1
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- 239000000411 inducer Substances 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
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- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- GWHOGODUVLQCEB-UHFFFAOYSA-N pyridine-2,6-dicarbonyl chloride Chemical compound ClC(=O)C1=CC=CC(C(Cl)=O)=N1 GWHOGODUVLQCEB-UHFFFAOYSA-N 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
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- 229910052712 strontium Inorganic materials 0.000 description 1
- 229910001631 strontium chloride Inorganic materials 0.000 description 1
- AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- -1 titanate anion Chemical class 0.000 description 1
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- 235000019871 vegetable fat Nutrition 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к способу глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз и может быть использовано в микробиологической промышленности для получения липолитических ферментов с широким применением в пищевой промышленности, производстве и переработке жиров и растительных масел, в медицине в качестве терапевтического и диагностического средства.Способ, согласно изобретению, предусматривает получение суспензии спор штамма выращенного в течении 30 дней на среде солод-агар, инокуляцию суспензии в количестве 5 об.% в питательную водную среду, содержащую, г/л: соевую муку - 35,0, (NH4)2SO4 - 1,0, KH2PO4 -5,0, с одновременным введением 0,005 г/л [SrL3][Co(NCS)4], где L - диметилпиридин-2,6-дикарбоксилат, и культивирование при постоянном перемешивания при 180-200 об/мин в течение 24 ч, при температуре 28-30°C.Результат изобретения состоит в увеличении биосинтеза липолитических ферментов и сокращении продолжительности культивирования штамма на 24 часа.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20210053A MD1624Z8 (ru) | 2021-06-23 | 2021-06-23 | Способ глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20210053A MD1624Z8 (ru) | 2021-06-23 | 2021-06-23 | Способ глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD1624Y MD1624Y (ru) | 2022-05-31 |
| MD1624Z true MD1624Z (ru) | 2022-12-31 |
| MD1624Z8 MD1624Z8 (ru) | 2024-01-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20210053A MD1624Z8 (ru) | 2021-06-23 | 2021-06-23 | Способ глубинного культивирования штамма гриба Rhizopus arrhizus CNMN FD 03, продуцента липаз |
Country Status (1)
| Country | Link |
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| MD (1) | MD1624Z8 (ru) |
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2021
- 2021-06-23 MD MDS20210053A patent/MD1624Z8/ru active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| MD1624Y (ru) | 2022-05-31 |
| MD1624Z8 (ru) | 2024-01-31 |
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