MD1072Z - Способ консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью - Google Patents
Способ консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью Download PDFInfo
- Publication number
- MD1072Z MD1072Z MDS20150151A MDS20150151A MD1072Z MD 1072 Z MD1072 Z MD 1072Z MD S20150151 A MDS20150151 A MD S20150151A MD S20150151 A MDS20150151 A MD S20150151A MD 1072 Z MD1072 Z MD 1072Z
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- MD
- Moldova
- Prior art keywords
- antifungal activity
- strain
- pseudomonas aureofaciens
- cnmn
- psb
- Prior art date
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- 230000000843 anti-fungal effect Effects 0.000 title claims abstract description 29
- 241001645955 Pseudomonas chlororaphis subsp. aureofaciens Species 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 12
- 239000005720 sucrose Substances 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 239000002609 medium Substances 0.000 description 14
- 230000001681 protective effect Effects 0.000 description 9
- 230000003032 phytopathogenic effect Effects 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 241000223602 Alternaria alternata Species 0.000 description 6
- 241000228245 Aspergillus niger Species 0.000 description 6
- 241000123650 Botrytis cinerea Species 0.000 description 6
- 241000233866 Fungi Species 0.000 description 6
- 241000223221 Fusarium oxysporum Species 0.000 description 6
- 241000427940 Fusarium solani Species 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 230000004936 stimulating effect Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 229940121375 antifungal agent Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 244000038559 crop plants Species 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000012225 czapek media Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к способу консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью.Способ, согласно изобретению, предусматривает культивирование Pseudomonas aureofaciens CNMN-PsB-07 на питательной среде в течение 48 часов при температуре 28…30°C, ресуспендирование в среде, состоящей из обезжиренного молока и 12% сахарозы с титром 10-8…10-9, быстрое замораживание при температуре -20°C, лиофилизацию и хранение при температуре + 4°C.Результат состоит в повышении антифунгальной активности штамма после хранения.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150151A MD1072Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150151A MD1072Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1072Y MD1072Y (ru) | 2016-09-30 |
| MD1072Z true MD1072Z (ru) | 2017-04-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20150151A MD1072Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Pseudomonas aureofaciens с антифунгальной активностью |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1072Z (ru) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1543C2 (ru) * | 1997-04-25 | 2001-03-31 | Ион АРСЕНЕ | Конвейерный ветродвигатель |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1093698A1 (ru) * | 1981-09-22 | 1984-05-23 | Одесский Ордена Трудового Красного Знамени Государственный Университет Им.И.И.Мечникова | Субстрат дл закреплени бактерий и способ хранени неспорообразующих бактерий рода @ |
| SU1671682A1 (ru) * | 1989-06-30 | 1991-08-23 | Институт Биохимии И Физиологии Микроорганизмов Ан Ссср | Способ хранени микроорганизмов |
| SU1698285A1 (ru) * | 1989-04-11 | 1991-12-15 | Всесоюзный научно-исследовательский институт прикладной энзимологии | Способ консервировани рекомбинантного штамма РSеUDомоNаS SpecIeS продуцента @ -2 интерферона |
| RU2123044C1 (ru) * | 1998-03-02 | 1998-12-10 | Открытое акционерное общество "Русский йогурт" | Способ длительного хранения естественных симбиотических ассоциаций микроорганизмов человека и животных. |
-
2015
- 2015-11-10 MD MDS20150151A patent/MD1072Z/ru not_active IP Right Cessation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1093698A1 (ru) * | 1981-09-22 | 1984-05-23 | Одесский Ордена Трудового Красного Знамени Государственный Университет Им.И.И.Мечникова | Субстрат дл закреплени бактерий и способ хранени неспорообразующих бактерий рода @ |
| SU1698285A1 (ru) * | 1989-04-11 | 1991-12-15 | Всесоюзный научно-исследовательский институт прикладной энзимологии | Способ консервировани рекомбинантного штамма РSеUDомоNаS SpecIeS продуцента @ -2 интерферона |
| SU1671682A1 (ru) * | 1989-06-30 | 1991-08-23 | Институт Биохимии И Физиологии Микроорганизмов Ан Ссср | Способ хранени микроорганизмов |
| RU2123044C1 (ru) * | 1998-03-02 | 1998-12-10 | Открытое акционерное общество "Русский йогурт" | Способ длительного хранения естественных симбиотических ассоциаций микроорганизмов человека и животных. |
Non-Patent Citations (2)
| Title |
|---|
| Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus. nr.3, 2015, p. 96-104 * |
| Slanina V., Lupaşcu L., Tolocichina S., Burţeva S., Postolachi O., Sîrbu T., Stepanov V., Chiseliţa O. Standardizarea procedurii de conservare a tulpinilor de bacterii păstrate in colecţia naţională de microorganisme nepatogene. I. Standardizarea procedurii de conservare a tulpinilor din genul Pseudomonas păstrate în colecţia naţională de microorganisme. Buletinul AŞM. Ştiinţele vieţii. Nr. 2(317) 2012, p160-167, găsit Internet <URL: <http://bsl.asm.md/article/id/22338> * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD1072Y (ru) | 2016-09-30 |
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