MD1071Z - Способ консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью - Google Patents
Способ консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью Download PDFInfo
- Publication number
- MD1071Z MD1071Z MDS20150150A MDS20150150A MD1071Z MD 1071 Z MD1071 Z MD 1071Z MD S20150150 A MDS20150150 A MD S20150150A MD S20150150 A MDS20150150 A MD S20150150A MD 1071 Z MD1071 Z MD 1071Z
- Authority
- MD
- Moldova
- Prior art keywords
- bacillus cereus
- strain
- antifungal activity
- cereus var
- fluorescens
- Prior art date
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- 230000000843 anti-fungal effect Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 13
- 239000005720 sucrose Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 239000002609 medium Substances 0.000 description 14
- 230000001681 protective effect Effects 0.000 description 9
- 241000223602 Alternaria alternata Species 0.000 description 7
- 241000228245 Aspergillus niger Species 0.000 description 7
- 241000123650 Botrytis cinerea Species 0.000 description 7
- 241000223221 Fusarium oxysporum Species 0.000 description 7
- 241000427940 Fusarium solani Species 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 230000003032 phytopathogenic effect Effects 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 229940121375 antifungal agent Drugs 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 239000012225 czapek media Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
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- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к способу консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью.Способ, согласно изобретению, предусматривает культивирование Bacillus cereus var. fluorescens CNMN-BB-07 на питательной среде в течение 48 часов при температуре 28…30°C, ресуспендирование в среде, состоящей из обезжиренного молока и 12% сахарозы с титром 10-8…10-9, быстрое замораживание при температуре -20°C, лиофилизацию и хранение при температуре + 4°C.Результат состоит в повышении антифунгальной активности штамма после хранения.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150150A MD1071Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150150A MD1071Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1071Y MD1071Y (ru) | 2016-09-30 |
| MD1071Z true MD1071Z (ru) | 2017-04-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| MDS20150150A MD1071Z (ru) | 2015-11-10 | 2015-11-10 | Способ консервирования штамма Bacillus cereus var. fluorescens с антифунгальной активностью |
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| MD (1) | MD1071Z (ru) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA49390C2 (en) * | 2001-11-28 | 2002-09-16 | Inst Agroecology And Biotechno | Strain of associative bacteria for bacillus polymyxa 6-m used for production of antigungal biological preparation |
| RU2514023C1 (ru) * | 2012-12-26 | 2014-04-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии Российской академии сельскохозяйственных наук (ГНУ ВНИИСХМ Россельхозакадемии) | Штамм bacillus thuringiensis var. darmstadiensis n 25 в качестве средства комплексного воздействия на вредных жесткокрылых насекомых и фитопатогенные грибы |
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2015
- 2015-11-10 MD MDS20150150A patent/MD1071Z/ru not_active IP Right Cessation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA49390C2 (en) * | 2001-11-28 | 2002-09-16 | Inst Agroecology And Biotechno | Strain of associative bacteria for bacillus polymyxa 6-m used for production of antigungal biological preparation |
| RU2514023C1 (ru) * | 2012-12-26 | 2014-04-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии Российской академии сельскохозяйственных наук (ГНУ ВНИИСХМ Россельхозакадемии) | Штамм bacillus thuringiensis var. darmstadiensis n 25 в качестве средства комплексного воздействия на вредных жесткокрылых насекомых и фитопатогенные грибы |
Non-Patent Citations (1)
| Title |
|---|
| Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus, nr. 3, 2015, p. 96-104 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD1071Y (ru) | 2016-09-30 |
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