MD1071Z - Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity - Google Patents
Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity Download PDFInfo
- Publication number
- MD1071Z MD1071Z MDS20150150A MDS20150150A MD1071Z MD 1071 Z MD1071 Z MD 1071Z MD S20150150 A MDS20150150 A MD S20150150A MD S20150150 A MDS20150150 A MD S20150150A MD 1071 Z MD1071 Z MD 1071Z
- Authority
- MD
- Moldova
- Prior art keywords
- bacillus cereus
- strain
- antifungal activity
- cereus var
- fluorescens
- Prior art date
Links
- 230000000843 anti-fungal effect Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 18
- 229930006000 Sucrose Natural products 0.000 claims abstract description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 13
- 239000005720 sucrose Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 11
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 239000002609 medium Substances 0.000 description 14
- 230000001681 protective effect Effects 0.000 description 9
- 241000223602 Alternaria alternata Species 0.000 description 7
- 241000228245 Aspergillus niger Species 0.000 description 7
- 241000123650 Botrytis cinerea Species 0.000 description 7
- 241000223221 Fusarium oxysporum Species 0.000 description 7
- 241000427940 Fusarium solani Species 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 230000003032 phytopathogenic effect Effects 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 244000005700 microbiome Species 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000004936 stimulating effect Effects 0.000 description 3
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 229940121375 antifungal agent Drugs 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 239000012225 czapek media Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Invenţia se referă la biotehnologie, şi anume la un procedeu de conservare a tulpinii Bacillus cereus var. fluorescens cu activitate antifungică, şi anume asupra diferitor tulpini de fungi fitopatogeni ca: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger ce provoacă diferite boli la plantele de cultură. Datorită capacităţii de sporire a activităţii antifungice, procedeul poate fi utilizat la conservarea microorganismelor pe un termen îndelungat şi utilizarea acestora ca agenţi de protejare a plantelor de cultură de infectarea cu fitopatogeni. The invention relates to biotechnology, namely to a process for preserving the strain Bacillus cereus var. fluorescens with antifungal activity, namely on different strains of phytopathogenic fungi such as: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger which cause various diseases in cultivated plants. Due to the ability to increase antifungal activity, the process can be used for long-term preservation of microorganisms and their use as agents for protecting cultivated plants from infection with phytopathogens.
Afectarea plantelor de cultură de către fitopatogeni duce la scăderea productivităţii lor, la micşorarea recoltei, precum şi la diminuarea calităţii produsului obţinut. Utilizarea microorganismelor în calitate de agenţi cu proprietăţi antimicrobiene pronunţate asupra fitopatogenilor permite atât majorarea productivităţii plantelor, cât şi îmbunătăţirea calităţii produsului. The damage to crop plants by phytopathogens leads to a decrease in their productivity, a decrease in the harvest, as well as a decrease in the quality of the product obtained. The use of microorganisms as agents with pronounced antimicrobial properties on phytopathogens allows both to increase plant productivity and to improve product quality.
În prezent este cunoscut un procedeu de stimulare a activităţii antifungice a tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 asupra fungilor fitopatogeni în urma liofilizării, utilizând ca mediu protector succinat de Na + 12% zaharoză, în rezultatul căruia, după 6 ani de păstrare, zonele de inhibiţie a patogenilor au atins valori de 15,0…25,0 mm, în dependenţă de test-cultura studiată [1]. Currently, a process is known for stimulating the antifungal activity of the Bacillus cereus var. fluorescens CNMN-BB-07 strain on phytopathogenic fungi following lyophilization, using Na succinate + 12% sucrose as a protective medium, as a result of which, after 6 years of storage, the pathogen inhibition zones reached values of 15.0…25.0 mm, depending on the test culture studied [1].
Dezavantajul acestui procedeu constă în aceea că proprietăţile antifungice sunt slabe şi mai puţin pronunţate după păstrarea îndelungată. The disadvantage of this process is that the antifungal properties are weak and less pronounced after prolonged storage.
Problema pe care o rezolvă prezenta invenţie constă în elaborarea unui procedeu de stimulare a activităţii antifungice a tulpinii Bacillus cereus var. fluorescens CNMN-BB-07, în urma liofilizării, cu utilizarea unui mediu protector ce asigură stimularea proprietăţilor antifungice asupra fitopatogenilor, după 6 ani de păstrare. The problem solved by the present invention consists in developing a process for stimulating the antifungal activity of the Bacillus cereus var. fluorescens CNMN-BB-07 strain, following lyophilization, with the use of a protective medium that ensures the stimulation of antifungal properties on phytopathogens, after 6 years of storage.
Procedeul revendicat constă în conservarea tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 cu capacitate antifungică prin liofilizarea acesteia, utilizând mediul de protecţie lapte degresat + 12% zaharoză. Zonele de inhibiţie a creşterii fitopatogenilor, după 6 ani de la conservare, ating valori de 31,0 mm pentru Fusarium oxysporum, 33,7 - Fusarium solani, 30,7 - Alternaria alternata, 28,7 - Botrytis cinerea şi 24,3 mm - Aspergillus niger. The claimed process consists in preserving the Bacillus cereus var. fluorescens CNMN-BB-07 strain with antifungal capacity by lyophilizing it, using the protective medium skimmed milk + 12% sucrose. The zones of inhibition of the growth of phytopathogens, after 6 years of preservation, reach values of 31.0 mm for Fusarium oxysporum, 33.7 - Fusarium solani, 30.7 - Alternaria alternata, 28.7 - Botrytis cinerea and 24.3 mm - Aspergillus niger.
Procedeul de conservare a tulpinii Bacillus cereus var. fluorescens cu activitate antifungică prevede cultivarea tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 pe un mediu nutritiv în decurs de 48 ore la temperatura de 28…30°C, resuspendarea într-un mediu constituit din lapte degresat şi 12% de zaharoză cu un titru de 10-8…10-9, congelarea rapidă la temperatura de -20°C, liofilizarea şi păstrarea la temperatura de + 4°C. The preservation process of the Bacillus cereus var. fluorescens strain with antifungal activity provides for the cultivation of the Bacillus cereus var. fluorescens strain CNMN-BB-07 on a nutrient medium within 48 hours at a temperature of 28…30°C, resuspension in a medium consisting of skimmed milk and 12% sucrose with a titer of 10-8…10-9, rapid freezing at a temperature of -20°C, lyophilization and storage at a temperature of + 4°C.
Rezultatul constă în majorarea activităţii antifungice a tulpinii după păstrare, şi anume stimularea evidentă a proprietăţilor antifungice asupra fitopatogenilor Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger după 6 ani de păstrare, acestea fiind mai înalte faţă de soluţia cea mai apropiată. Zonele de inhibiţie a creşterii fitopatogenilor obţinute în rezultatul liofilizării, cu utilizarea mediului protector succinat de Na + 12% zaharoză (soluţia cea mai apropiată), variază de la 15,0…25,0 mm pentru tulpinile F. oxysporum, F. solani, A. alternata B. cinerea şi A. niger, iar zona de inhibiţie determinată cu utilizarea procedeului propus în invenţie variază între 24,3…33,7 mm, ceea ce depăşeşte de până la 1,62 ori caracteristicile analoage obţinute conform celei mai apropiate soluţii. The result is the increase in the antifungal activity of the strain after storage, namely the obvious stimulation of the antifungal properties on the phytopathogens Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger after 6 years of storage, which are higher than the closest solution. The zones of inhibition of the growth of phytopathogens obtained as a result of lyophilization, using the protective medium Na succinate + 12% sucrose (the closest solution), vary from 15.0…25.0 mm for the strains F. oxysporum, F. solani, A. alternata B. cinerea and A. niger, and the zone of inhibition determined using the method proposed in the invention varies between 24.3…33.7 mm, which exceeds by up to 1.62 times the analogous characteristics obtained according to the closest solution.
Rezultatul tehnic al invenţiei este condiţionat de faptul că, în calitate de mediu protector pentru liofilizarea tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 se utilizează lapte degresat + 12% zaharoză. Proprietăţile antifungice evidente sunt asigurate datorită resuspendării tulpinii în mediul dat, care asigură un efect protector faţă de şocurile osmotice şi termice şi nu permite acestora de a influenţa negativ sinteza metaboliţilor cu activitate antifungică. The technical result of the invention is conditioned by the fact that, as a protective medium for lyophilization of the strain Bacillus cereus var. fluorescens CNMN-BB-07, skimmed milk + 12% sucrose is used. The evident antifungal properties are ensured due to the resuspension of the strain in the given medium, which provides a protective effect against osmotic and thermal shocks and does not allow them to negatively influence the synthesis of metabolites with antifungal activity.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Se prepară mediul nutritiv agarizat King B cu următorul conţinut al ingredientelor: peptonă - 20,0 g; glicerină - 15,0 mL; K2HPO4 - 1,5 g; MgSO4·7H2O - 1,5 g; agar - 15,0 g; apă distilată până la 1,0 litru; pH-ul = 7,0 - 7,2. Pe mediul preparat, în tuburi înclinate, se reînsămânţează tulpina Bacillus cereus var. fluorescens CNMN-BB-07, după 48 ore de incubare la +28…30°C, cultura se resuspendă în mediul protector lapte degresat + 12% zaharoză până la titrul de 10-8…10-9, se repartizează câte 1,0…2,0 mL de suspensie în flacoane şi se congelează rapid la -20°C, după care probele se liofilizează. Probele liofilizate sunt sigilate şi plasate în frigider la temperatura de +4°C. După 6 ani de conservare în stare liofilizată tulpina se reînsămânţează pentru a determina activitatea antifungică. Prepare the King B agarized nutrient medium with the following content of ingredients: peptone - 20.0 g; glycerin - 15.0 mL; K2HPO4 - 1.5 g; MgSO4·7H2O - 1.5 g; agar - 15.0 g; distilled water up to 1.0 liter; pH = 7.0 - 7.2. On the prepared medium, in inclined tubes, the Bacillus cereus var. fluorescens CNMN-BB-07 strain is re-seeded, after 48 hours of incubation at +28…30°C, the culture is resuspended in the protective medium skimmed milk + 12% sucrose up to a titer of 10-8…10-9, 1.0…2.0 mL of suspension is distributed into vials and quickly frozen at -20°C, after which the samples are lyophilized. The lyophilized samples are sealed and placed in a refrigerator at +4°C. After 6 years of preservation in a lyophilized state, the strain is reseeded to determine antifungal activity.
Paralel se pregătesc culturile de fungi fitopatogeni care sunt inoculate pe mediul Czapek cu următoarea componenţă (g/L): zaharoză - 30,0; NaNO3 - 3,0; KH2PO4 - 1,0; MgSO4·7H2O - 0,5; KCl - 0,5; FeSO4 - 0,01; agar - 20,0; apă distilată până la 1,0 litru; pH-ul = 7,0…7,2. Suspensia de spori a fungilor fitopatogeni cu titrul 10-7…10-8 se reînsămânţează submers în plăcile Petri. In parallel, phytopathogenic fungi cultures are prepared and inoculated on Czapek medium with the following composition (g/L): sucrose - 30.0; NaNO3 - 3.0; KH2PO4 - 1.0; MgSO4·7H2O - 0.5; KCl - 0.5; FeSO4 - 0.01; agar - 20.0; distilled water up to 1.0 liter; pH = 7.0…7.2. The spore suspension of phytopathogenic fungi with a titer of 10-7…10-8 is reseeded submerged in Petri dishes.
Determinarea activităţii antifungice a tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 după 6 ani de la liofilizare pe mediul protector lapte degresat + 12% zaharoză a fost efectuată conform metodei de difuziune a metaboliţilor prin utilizarea discurilor de geloză. În calitate de culturi de referinţă în experimentul in vitro au fost folosite tulpinile fitopatogene de micromicete: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger. După 4…5 zile de incubare se măsoară zonele de inhibiţie aparente în jurul discurilor de geloză. Determination of the antifungal activity of the Bacillus cereus var. fluorescens CNMN-BB-07 strain after 6 years of lyophilization on the protective medium skimmed milk + 12% sucrose was performed according to the metabolite diffusion method using agarose discs. As reference cultures in the in vitro experiment, the phytopathogenic strains of micromycetes were used: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger. After 4…5 days of incubation, the apparent inhibition zones around the agar discs were measured.
Proprietăţile antifungice pronunţate depistate în urma elaborării procedeului de conservare şi stimulare a activităţii antifungice a tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 asupra fitopatogenilor prezintă interes din punct de vedere al extinderii arsenalului de microorganisme cu capacitate antifungică pronunţată, cu utilizarea acestora ca agenţi de prevenire şi protejare a plantelor de cultură. The pronounced antifungal properties detected following the development of the process for preserving and stimulating the antifungal activity of the Bacillus cereus var. fluorescens CNMN-BB-07 strain on phytopathogens are of interest from the point of view of expanding the arsenal of microorganisms with pronounced antifungal capacity, with their use as agents for the prevention and protection of cultivated plants.
Rezultatele studiului activităţii antifungice după 6 ani de conservare, în urma liofilizării tulpinii Bacillus cereus var. fluorescens CNMN-BB-07 utilizând ca mediu protector laptele degresat + 12% zaharoză, sunt prezentate în tabel, care relevă o activitate mai sporită faţă de cea mai apropiată soluţie. Astfel, datele experimentale obţinute demonstrează că procedeul revendicat manifestă activitate antifungică mai sporită faţă de fitopatogenii Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger, care depăşeşte de până la 1,62 ori caracteristicile analoage ale procedeului descris în cea mai apropiată soluţie. The results of the study of the antifungal activity after 6 years of preservation, following lyophilization of the Bacillus cereus var. fluorescens CNMN-BB-07 strain using skimmed milk + 12% sucrose as a protective medium, are presented in the table, which reveals a higher activity compared to the closest solution. Thus, the experimental data obtained demonstrate that the claimed process exhibits higher antifungal activity against the phytopathogens Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger, which exceeds by up to 1.62 times the analogous characteristics of the process described in the closest solution.
Tabel Table
Activitatea antifungică a tulpinii Bacillus cereus var. fluorescens CNMN-BB-07, după 6 ani de conservare în stare liofilizată conform procedeului propus în invenţie şi celei mai apropiate soluţii Antifungal activity of the Bacillus cereus var. fluorescens strain CNMN-BB-07, after 6 years of preservation in a lyophilized state according to the process proposed in the invention and the closest solution
Metoda utilizată Zona de inhibiţie a fungilor fitopatogeni (mm) Fusarium oxysporum Fusarium solani Alternaria alternata Botrytis cinerea Aspergillus niger Conform celei mai apropiate soluţii Liofilizarea pe mediul succinat de Na + 12% zaharoză 23,0±1,1 25,0±1,1 19,3±1,3 23,7±1,7 15,0±1,1 Conform soluţiei propuse în invenţie Liofilizarea pe mediul lapte degresat + 12% zaharoză 31,0±1,1 33,7±1,7 30,7±1,3 28,7±2,4 24,3±1,7Method used Inhibition zone of phytopathogenic fungi (mm) Fusarium oxysporum Fusarium solani Alternaria alternata Botrytis cinerea Aspergillus niger According to the closest solution Lyophilization on Na succinate medium + 12% sucrose 23.0±1.1 25.0±1.1 19.3±1.3 23.7±1.7 15.0±1.1 According to the solution proposed in the invention Lyophilization on skim milk medium + 12% sucrose 31.0±1.1 33.7±1.7 30.7±1.3 28.7±2.4 24.3±1.7
Datele din tabel demonstrează că zonele de inhibiţie determinate conform procedeului propus în invenţie sunt mai mari şi le depăşesc de până la 1,62 ori pe cele descrise în cea mai apropiată soluţie. The data in the table demonstrate that the inhibition zones determined according to the method proposed in the invention are larger and exceed by up to 1.62 times those described in the closest solution.
Proprietăţile depistate ale tulpinii Bacillus cereus var. fluorescens CNMN-BB-07, liofilizată conform procedeului revendicat, prezintă interes din punct de vedere al extinderii arsenalului de preparate cu acţiune antifungică pronunţată asupra fungilor fitopatogeni - dăunători ai plantelor de cultură. The discovered properties of the Bacillus cereus var. fluorescens strain CNMN-BB-07, lyophilized according to the claimed process, are of interest from the point of view of expanding the arsenal of preparations with pronounced antifungal action on phytopathogenic fungi - pests of cultivated plants.
1. Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus, nr. 3, 2015, p. 96-104 1. Batîr L., Slanina V., Sârbu T., Chiseliţa O. Antifungal activity of some indigenous strains of microorganisms after 3 and 6 years of preservation in lyophilized state. Intelectus Intellectual Property Journal, no. 3, 2015, p. 96-104
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150150A MD1071Z (en) | 2015-11-10 | 2015-11-10 | Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20150150A MD1071Z (en) | 2015-11-10 | 2015-11-10 | Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1071Y MD1071Y (en) | 2016-09-30 |
| MD1071Z true MD1071Z (en) | 2017-04-30 |
Family
ID=57014931
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20150150A MD1071Z (en) | 2015-11-10 | 2015-11-10 | Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1071Z (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA49390C2 (en) * | 2001-11-28 | 2002-09-16 | Inst Agroecology And Biotechno | Strain of associative bacteria for bacillus polymyxa 6-m used for production of antigungal biological preparation |
| RU2514023C1 (en) * | 2012-12-26 | 2014-04-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии Российской академии сельскохозяйственных наук (ГНУ ВНИИСХМ Россельхозакадемии) | Strain bacillus thuringiensis var darmstadiensis n25 as means of integrated effect on harmful coleopteran insects and phytopathogenic fungi |
-
2015
- 2015-11-10 MD MDS20150150A patent/MD1071Z/en not_active IP Right Cessation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA49390C2 (en) * | 2001-11-28 | 2002-09-16 | Inst Agroecology And Biotechno | Strain of associative bacteria for bacillus polymyxa 6-m used for production of antigungal biological preparation |
| RU2514023C1 (en) * | 2012-12-26 | 2014-04-27 | Государственное научное учреждение Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии Российской академии сельскохозяйственных наук (ГНУ ВНИИСХМ Россельхозакадемии) | Strain bacillus thuringiensis var darmstadiensis n25 as means of integrated effect on harmful coleopteran insects and phytopathogenic fungi |
Non-Patent Citations (1)
| Title |
|---|
| Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus, nr. 3, 2015, p. 96-104 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD1071Y (en) | 2016-09-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | Volatile organic compounds (VOCs) from Bacillus subtilis CF-3 reduce anthracnose and elicit active defense responses in harvested litchi fruits | |
| Yu et al. | Effect of chitin on the antagonistic activity of Cryptococcus laurentii against Penicillium expansum in pear fruit | |
| Jones et al. | Biocontrol of Sclerotinia sclerotiorum infection of cabbage by Coniothyrium minitans and Trichoderma spp. | |
| Yang et al. | Antifungal activity and mechanism of tea polyphenols against Rhizopus stolonifer | |
| Çorbacı et al. | Purification, characterization and in vivo biocontrol efficiency of killer toxins from Debaryomyces hansenii strains | |
| Ma et al. | Purification and characterization of chitinase from Gliocladium catenulatum strain HL-1-1 | |
| Wachid | Inhibitory Power Test of Two Trichoderma Isolates in In Vitro Way Againts Fusarium oxysporum The Cause of Red Chilli Stem Rot | |
| Agrillo et al. | An alternative biocontrol agent of soil-borne phytopathogens: A new antifungal compound produced by a plant growth promoting bacterium isolated from North Algeria | |
| Magallon-Andalon et al. | Parasitism and substrate competitions effect of antagonistic yeasts for biocontrol of Colletotrichum gloeosporioides in papaya (Carica papaya L.) var Maradol | |
| Talib et al. | Isolation, culture, and maintenance of Extremophilic fungi | |
| Guo et al. | Effect of antagonistic yeast Pichia membranaefaciens on black spot decay of postharvest broccoli | |
| MD1071Z (en) | Process for conservation of Bacillus cereus var. fluorescens strain with antifungal activity | |
| MD1072Z (en) | Process for conservation of Pseudomonas aureofaciens strain with antifungal activity | |
| MD1056Z (en) | Process for conservation of Pseudomonas aurantiaca strain with antifungal activity | |
| Zargaryan et al. | Infection of grain crops with fungi of the genus Fusarium | |
| Minamor et al. | Influence of the culture filtrate of three Paecilomyces species on some growth parameters, chlorophyll content, and root anatomy of two Ghanaian maize varieties (Abeleehi and Obaatanpa) and on germination capacity of tomato and pepper seeds | |
| KR101481897B1 (en) | Streptomyces sp. BS063 and biological control of plant pathogenic fungi by using the same | |
| Summerell et al. | Factors influencing survival of Pyrenophora tritici-repentis: water potential and temperature | |
| Jankiewicz et al. | Purification, characteristics and identification of chitinases synthesized by the bacterium Serratia plymuthica MP44 antagonistic against phytopathogenic fungi | |
| Ghewande | Biological control of groundnut (Arachis hypogaea L.) rust (Puccinia arachidis Speg.) in India | |
| Ji et al. | Biocontrol potential of Bacillus licheniformis W10 against peach shoot blight caused by Phomopsis amygdali | |
| Zhu et al. | Biocontrol efficiency and multiple mechanisms of Bacillus velezensis BV07 against postharvest green mold on citrus fruit | |
| CN103937689A (en) | Pseudozyma fusiformata for inhibiting diseases after fresh fruit collection and application thereof | |
| Goswami et al. | Aspergillus niger, a dominant phylloplane coloniser, influences the activity of defense enzymes in Solanum lycopersicum | |
| de Almeida et al. | In vitro control of phytopathogenic fungi and damping-off of tomato by Bacillus velezensis LABIM40 (CMRP 4489) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| MK4Y | Short term patent expired |