MD1056Z - Process for conservation of Pseudomonas aurantiaca strain with antifungal activity - Google Patents
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- MD1056Z MD1056Z MDS20150149A MDS20150149A MD1056Z MD 1056 Z MD1056 Z MD 1056Z MD S20150149 A MDS20150149 A MD S20150149A MD S20150149 A MDS20150149 A MD S20150149A MD 1056 Z MD1056 Z MD 1056Z
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- pseudomonas aurantiaca
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- 230000000843 anti-fungal effect Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241001670013 Pseudomonas chlororaphis subsp. aurantiaca Species 0.000 title claims abstract description 17
- 229930006000 Sucrose Natural products 0.000 claims abstract description 13
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 13
- 239000005720 sucrose Substances 0.000 claims abstract description 13
- 238000004108 freeze drying Methods 0.000 claims abstract description 12
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 241000589516 Pseudomonas Species 0.000 abstract description 3
- 238000004448 titration Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 14
- 241000228245 Aspergillus niger Species 0.000 description 9
- 230000001681 protective effect Effects 0.000 description 9
- 241000223602 Alternaria alternata Species 0.000 description 8
- 241000123650 Botrytis cinerea Species 0.000 description 8
- 241000223221 Fusarium oxysporum Species 0.000 description 8
- 241000427940 Fusarium solani Species 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 8
- 230000003032 phytopathogenic effect Effects 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 230000004936 stimulating effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 239000012225 czapek media Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008659 phytopathology Effects 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Invenţia se referă la biotehnologie, şi anume la un procedeu de conservare a tulpinii Pseudomonas auraniaca cu activitate antifungică.Procedeul, conform invenţiei, prevede cultivarea tulpinii Pseudomonas aurantiaca CNMN-PsB-08 pe un mediu nutritiv în decurs de 48 ore la temperatura de 28…30°C, resuspendarea într-un mediu constituit din lapte degresat şi 12% de zaharoză cu un titru de 10-8…10 -9, congelarea rapidă la temperatura de -20°C, liofilizarea şi păstrarea la temperatura de + 4°C.Rezultatul constă în majorarea activităţii antifungice a tulpinii după păstrare.The invention relates to biotechnology, namely to a process for the conservation of the Pseudomonas auraniaca strain with antifungal activity. The process according to the invention provides for cultivation of the Pseudomonas aurantiaca CNMN-PsB-08 strain on a nutrient medium within 48 hours at a temperature of 28 ... 30 ° C, resuspension in an environment consisting of skim milk and 12% sucrose with a titration of 10-8 ... 10 -9, rapid freezing at -20 ° C, lyophilization and storage at + 4 ° C .The result is an increase in the antifungal activity of the strain after storage.
Description
Invenţia se referă la biotehnologie, şi anume la un procedeu de conservare a tulpinii Pseudomonas auraniaca cu activitate antifungică. The invention relates to biotechnology, namely to a process for preserving the Pseudomonas auraniaca strain with antifungal activity.
Invenţia poate fi utilizată în fitopatologie, şi anume pentru stimularea activităţii antifungice a tulpinii Pseudomonas aurantiaca CNMN-PsB-08 asupra fungilor fitopatogeni ca: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger ce provoacă diferite boli la plantele de cultură. Datorită capacităţii de păstrare şi sporire a activităţii antifungice, la conservarea microorganismelor pe un termen îndelungat, utilizarea acestora ca agenţi de protejare a plantelor de cultură previne infectarea cu fitopatogeni. The invention can be used in phytopathology, namely for stimulating the antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08 on phytopathogenic fungi such as: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger which cause various diseases in cultivated plants. Due to the ability to preserve and increase the antifungal activity, when preserving microorganisms for a long time, their use as agents for protecting cultivated plants prevents infection with phytopathogens.
Afectarea plantelor de cultură de către fitopatogeni duce la scăderea productivităţii lor, micşorarea recoltei şi diminuarea calităţii produsului obţinut. Astfel, utilizarea microorganismelor în calitate de agenţi cu proprietăţi antimicrobiene pronunţate asupra fitopatogenilor ar permite atât majorarea productivităţii plantelor, cât şi îmbunătăţirea calităţii produsului, metoda dovedindu-se a fi eficientă şi ecologică. The damage of crop plants by phytopathogens leads to a decrease in their productivity, a decrease in the harvest and a decrease in the quality of the product obtained. Thus, the use of microorganisms as agents with pronounced antimicrobial properties on phytopathogens would allow both an increase in plant productivity and an improvement in product quality, the method proving to be efficient and ecological.
În prezent este cunoscut un procedeu de stimulare a activităţii antifungice a tulpinii Pseudomonas aurantiaca CNMN-PsB-08 asupra fungilor fitopatogeni în rezultatul liofilizării utilizând ca mediu protector succinat de Na + 12% zaharoză. În urma realizării procedeului, după 6 ani de păstrare, zonele de inhibiţie a patogenilor au atins valori de 21,3…23,0 mm, în dependenţă de test-cultura studiată. Dezavantajul procedeului constă în aceea că proprietăţile antifungice ale tulpinii sunt relativ slabe după păstrarea îndelungată [1]. Currently, a process for stimulating the antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08 on phytopathogenic fungi as a result of lyophilization using Na succinate + 12% sucrose as a protective medium is known. Following the implementation of the process, after 6 years of storage, the pathogen inhibition zones reached values of 21.3…23.0 mm, depending on the test culture studied. The disadvantage of the process is that the antifungal properties of the strain are relatively weak after long-term storage [1].
Problema pe care o rezolvă prezenta invenţie constă în stimularea activităţii antifungice a tulpinii Pseudomonas aurantiaca CNMN-PsB-08, în urma liofilizării, cu utilizarea unui mediu protector ce asigură stimularea proprietăţilor antifungice asupra fitopatogenilor, după 6 ani de păstrare. The problem solved by the present invention consists in stimulating the antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08, following lyophilization, with the use of a protective medium that ensures the stimulation of antifungal properties on phytopathogens, after 6 years of storage.
Procedeul de conservare a tulpinii Pseudomonas auraniaca cu activitate antifungică prevede cultivarea tulpinii Pseudomonas aurantiaca CNMN-PsB-08 pe un mediu nutritiv în decurs de 48 ore la temperatura de 28…30°C, resuspendarea într-un mediu constituit din lapte degresat şi 12% de zaharoză cu un titru de 10-8…10-9, congelarea rapidă la temperatura de -20°C, liofilizarea şi păstrarea la temperatura de + 4°C. The preservation process of the Pseudomonas auraniaca strain with antifungal activity involves cultivating the Pseudomonas aurantiaca strain CNMN-PsB-08 on a nutrient medium for 48 hours at a temperature of 28…30°C, resuspension in a medium consisting of skimmed milk and 12% sucrose with a titer of 10-8…10-9, rapid freezing at a temperature of -20°C, lyophilization and storage at a temperature of + 4°C.
Rezultatul constă în majorarea activităţii antifungice a tulpinii după păstrare. The result is an increase in the antifungal activity of the strain after storage.
Rezultatul se datorează liofilizării tulpinii Pseudomonas aurantiaca CNMN-PsB-08, utilizând mediul de protecţie lapte degresat + 12% zaharoză. Zonele de inhibiţie a creşterii fitopatogenilor, după 6 ani de la conservare, ating valori de 25,7 mm pentru Fusarium oxysporum, 26,0 - Fusarium solani, 25,3 - Alternaria alternata, 27,0 - Botrytis cinerea şi 20,0 mm - Aspergillus niger. The result is due to the lyophilization of the Pseudomonas aurantiaca strain CNMN-PsB-08, using the protective medium skimmed milk + 12% sucrose. The zones of inhibition of the growth of phytopathogens, after 6 years of conservation, reach values of 25.7 mm for Fusarium oxysporum, 26.0 - Fusarium solani, 25.3 - Alternaria alternata, 27.0 - Botrytis cinerea and 20.0 mm - Aspergillus niger.
Rezultatul constă în stimularea activităţii antifungice a tulpinii Pseudomonas aurantiaca CNMN-PsB-08 care permite amplificarea evidentă a proprietăţilor antifungice asupra fitopatogenilor Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger după 6 ani de păstrare, acestea fiind mai înalte faţă de cea mai apropiată soluţie. Zonele de inhibiţie a creşterii fitopatogenilor obţinute în rezultatul liofilizării cu utilizarea mediului protector succinat de Na + 12% zaharoză, cea mai apropiată soluţie, variază de la 21,3…23,0 mm pentru tulpinile F. oxysporum, F. solani, A. alternata şi B. cinerea şi lipseşte pentru tulpina A. niger, iar zonele de inhibiţie determinate cu utilizarea procedeului propus în invenţie variază între 25,3…27,0 mm pentru tulpinile F. oxysporum, F. solani, A. alternata şi B. cinerea şi 20,0 mm pentru tulpina A. niger, ceea ce depăşeşte de până la 1,2 ori caracteristicile analoage obţinute conform prototipului. The result consists in the stimulation of the antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08 which allows the obvious amplification of the antifungal properties on the phytopathogens Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger after 6 years of storage, these being higher than the closest solution. The zones of inhibition of the growth of phytopathogens obtained as a result of lyophilization using the protective medium Na succinate + 12% sucrose, the closest solution, vary from 21.3…23.0 mm for the strains F. oxysporum, F. solani, A. alternata and B. cinerea and are absent for the strain A. niger, and the zones of inhibition determined using the method proposed in the invention vary between 25.3…27.0 mm for the strains F. oxysporum, F. solani, A. alternata and B. cinerea and 20.0 mm for the strain A. niger, which exceeds by up to 1.2 times the analogous characteristics obtained according to the prototype.
Rezultatul tehnic al invenţiei este condiţionat de faptul că pentru prima dată în calitate de mediu protector pentru liofilizarea tulpinii Pseudomonas aurantiaca CNMN-PsB-08 se utilizează laptele degresat + 12% zaharoză. Proprietăţile antifungice evidente sunt asigurate prin resuspendarea tulpinii în mediul dat, care asigură un efect protector faţă de şocurile osmotice şi termice şi nu permite acestora de a influenţa negativ sinteza metaboliţilor cu activitate antifungică. The technical result of the invention is conditioned by the fact that for the first time as a protective medium for lyophilization of the Pseudomonas aurantiaca CNMN-PsB-08 strain, skimmed milk + 12% sucrose is used. The evident antifungal properties are ensured by resuspending the strain in the given medium, which provides a protective effect against osmotic and thermal shocks and does not allow them to negatively influence the synthesis of metabolites with antifungal activity.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Se prepară mediul nutritiv agarizat King B cu următorul conţinut al ingredientelor: peptonă - 20,0 g, glicerină - 15,0 ml, K2HPO4 - 1,5 g, MgSO4·7H2O - 1,5 g, agar - 15,0 g, apă distilată până la 1,0 litru, pH-ul = 7,0…7,2. Pe mediul preparat, în tuburi înclinate, se însămânţează tulpina Pseudomonas aurantiaca CNMN-PsB-08, după 48 ore de incubare la temperatura de 28…30°C, cultura se resuspendă în mediul protector lapte degresat + 12% zaharoză până la titrul de 10-8…10-9, se repartizează câte 1,0…2,0 m de suspensie în flacoane şi se congelează rapid la -20°C, după care probele se liofilizează. Probele liofilizate sunt sigilate şi plasate în frigider la temperatura de +4°C. După 6 ani de conservare în stare liofilizată tulpina se reînsămânţează pentru a determina activitatea antifungică. Prepare the King B agarized nutrient medium with the following content of ingredients: peptone - 20.0 g, glycerin - 15.0 ml, K2HPO4 - 1.5 g, MgSO4·7H2O - 1.5 g, agar - 15.0 g, distilled water up to 1.0 liter, pH = 7.0…7.2. On the prepared medium, in inclined tubes, the Pseudomonas aurantiaca strain CNMN-PsB-08 is inoculated, after 48 hours of incubation at a temperature of 28…30°C, the culture is resuspended in the protective medium skimmed milk + 12% sucrose up to a titer of 10-8…10-9, 1.0…2.0 m of suspension is distributed into vials and quickly frozen at -20°C, after which the samples are lyophilized. The lyophilized samples are sealed and placed in a refrigerator at a temperature of +4°C. After 6 years of preservation in a lyophilized state, the strain is reseeded to determine antifungal activity.
Paralel se pregătesc culturile de fungi fitopatogeni care sunt inoculate pe mediul Czapek cu următoarea componenţă (g/L): zaharoză - 30,0, NaNO3 - 3,0, KH2PO4 - 1,0, MgSO4·7H2O - 0,5, KCl - 0,5, FeSO4 - 0,01, agar - 20,0, apă distilată până la 1,0 litru, pH-ul = 7,0…7,2. Suspensia de spori a fungilor fitopatogeni cu titrul 10-7…10-8 se reînsămânţează submers în plăcile Petri. In parallel, phytopathogenic fungi cultures are prepared and inoculated on Czapek medium with the following composition (g/L): sucrose - 30.0, NaNO3 - 3.0, KH2PO4 - 1.0, MgSO4·7H2O - 0.5, KCl - 0.5, FeSO4 - 0.01, agar - 20.0, distilled water up to 1.0 liter, pH = 7.0…7.2. The spore suspension of phytopathogenic fungi with a titer of 10-7…10-8 is reseeded submerged in Petri dishes.
Activitatea antifungică a tulpinii Pseudomonas aurantiaca CNMN-PsB-08 după 6 ani de la liofilizare pe mediul protector lapte degresat + 12% zaharoză a fost determinată conform metodei de difuziune a metaboliţilor prin utilizarea discurilor de geloză. În calitate de culturi de referinţă în experimentul ,,in vitro” au fost folosite tulpinile fitopatogene de micromicete: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger. După 4…5 zile de incubare se măsoară zonele de inhibiţie, aparente în jurul discurilor de geloză. The antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08 after 6 years of lyophilization on the protective medium skimmed milk + 12% sucrose was determined according to the metabolite diffusion method using agar discs. As reference cultures in the "in vitro" experiment, the phytopathogenic strains of micromycetes were used: Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger. After 4...5 days of incubation, the inhibition zones, apparent around the agar discs, were measured.
Proprietăţile antifungice, depistate în urma elaborării procedeului de conservare şi stimulare a activităţii antifungice a tulpinii Pseudomonas aurantiaca CNMN-PsB-08 asupra fitopatogenilor, prezintă interes din punct de vedere al extinderii arsenalului de microorganisme cu capacitate antifungică pronunţată, cu utilizarea acestora ca agenţi de prevenire şi protejare a plantelor de cultură. The antifungal properties, discovered following the development of the process for preserving and stimulating the antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08 on phytopathogens, are of interest from the point of view of expanding the arsenal of microorganisms with pronounced antifungal capacity, with their use as agents for the prevention and protection of cultivated plants.
Rezultatele studiului activităţii antifungice după 6 ani de conservare, în urma liofilizării tulpinii Pseudomonas aurantiaca CNMN-PsB-08 utilizând ca mediu protector laptele degresat + 12% zaharoză, sunt prezentate în tabel, care relevă o activitate mai sporită faţă de cea mai apropiată soluţie. Astfel, datele experimentale obţinute demonstrează că procedeul revendicat manifestă activitate antifungică mai sporită faţă de fitopatogenii Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea şi Aspergillus niger, care depăşeşte de până la 1,2 ori caracteristicile analoage ale procedeului descris în cea mai apropiată soluţie. The results of the study of antifungal activity after 6 years of preservation, following lyophilization of the Pseudomonas aurantiaca strain CNMN-PsB-08 using skimmed milk + 12% sucrose as a protective medium, are presented in the table, which reveals a higher activity compared to the closest solution. Thus, the experimental data obtained demonstrate that the claimed process exhibits higher antifungal activity against the phytopathogens Fusarium oxysporum, Fusarium solani, Alternaria alternata, Botrytis cinerea and Aspergillus niger, which exceeds by up to 1.2 times the analogous characteristics of the process described in the closest solution.
Tabel Table
Activitatea antifungică a tulpinii Pseudomonas aurantiaca CNMN-PsB-08, după 6 ani de conservare în stare liofilizată conform invenţiei şi celei mai apropiate soluţii Antifungal activity of the Pseudomonas aurantiaca strain CNMN-PsB-08, after 6 years of preservation in a lyophilized state according to the invention and the closest solution
Procedeul utilizat Zona de inhibiţie a fungilor fitopatogeni (mm) Fusarium oxysporum Fusarium solani Alternaria alternata Botrytis cinerea Aspergillus niger Conform celei mai apropiate soluţii Liofilizarea pe mediul succinat de Na + 12% zaharoză 21,3±0,7 22,0±1,1 21,3±0,7 23,0±1,1 - Conform procedeului propus Liofilizarea pe mediul lapte degresat + 12% zaharoză 25,7±2,4 26,0±1,1 25,3±0,7 27,0±1,1 20,0±1,1Process used Zone of inhibition of phytopathogenic fungi (mm) Fusarium oxysporum Fusarium solani Alternaria alternata Botrytis cinerea Aspergillus niger According to the closest solution Lyophilization on Na succinate medium + 12% sucrose 21.3±0.7 22.0±1.1 21.3±0.7 23.0±1.1 - According to the proposed process Lyophilization on skim milk medium + 12% sucrose 25.7±2.4 26.0±1.1 25.3±0.7 27.0±1.1 20.0±1.1
Datele din tabel demonstrează că zonele de inhibiţie determinate conform procedeului propus sunt mai mari şi le depăşesc de până la 1,2 ori pe cele descrise conform celei mai apropiate soluţii, iar pentru tulpina Aspergillus niger zona de inhibiţie lipseşte în cazul utilizării celei mai apropiate soliţii şi atinge valoarea de 20,0 mm la utilizarea procedeului propus în invenţie. The data in the table demonstrate that the inhibition zones determined according to the proposed process are larger and exceed by up to 1.2 times those described according to the closest solution, and for the Aspergillus niger strain the inhibition zone is absent when using the closest solution and reaches the value of 20.0 mm when using the process proposed in the invention.
Proprietăţile depistate ale tulpinii Pseudomonas aurantiaca CNMN-PsB-08 liofilizate, conform procedeului revendicat, prezintă interes din punct de vedere al extinderii arsenalului de preparate cu acţiune antifungică pronunţată asupra fungilor fitopatogeni, dăunători ai plantelor de cultură. The discovered properties of the lyophilized Pseudomonas aurantiaca strain CNMN-PsB-08, according to the claimed process, are of interest from the point of view of expanding the arsenal of preparations with pronounced antifungal action on phytopathogenic fungi, pests of cultivated plants.
1. Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus. nr.3, 2015, p. 96-104 1. Batîr L., Slanina V., Sârbu T., Chiseliţa O. Antifungal activity of some indigenous strains of microorganisms after 3 and 6 years of preservation in lyophilized state. Intellectual Property Journal Intelectus. no. 3, 2015, p. 96-104
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1698285A1 (en) * | 1989-04-11 | 1991-12-15 | Всесоюзный научно-исследовательский институт прикладной энзимологии | Method to conserve recombinant pseudomonas species strain of @@@-interferon producent |
| RU2123044C1 (en) * | 1998-03-02 | 1998-12-10 | Открытое акционерное общество "Русский йогурт" | Method of the prolonged storage of natural symbiotic of human and animal microorganism associations |
| MD865Y (en) * | 2014-06-30 | 2015-01-31 | Instituţia Publică Institutul Ştiinţifico-Practic De Horticultură Şi Tehnologii Alimentare | Process for producing a powdery fermented dairy product |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1698285A1 (en) * | 1989-04-11 | 1991-12-15 | Всесоюзный научно-исследовательский институт прикладной энзимологии | Method to conserve recombinant pseudomonas species strain of @@@-interferon producent |
| RU2123044C1 (en) * | 1998-03-02 | 1998-12-10 | Открытое акционерное общество "Русский йогурт" | Method of the prolonged storage of natural symbiotic of human and animal microorganism associations |
| MD865Y (en) * | 2014-06-30 | 2015-01-31 | Instituţia Publică Institutul Ştiinţifico-Practic De Horticultură Şi Tehnologii Alimentare | Process for producing a powdery fermented dairy product |
Non-Patent Citations (1)
| Title |
|---|
| Batîr L., Slanina V., Sârbu T., Chiseliţa O. Activitatea antifungică a unor tulpini autohtone de microorganisme după 3 şi 6 ani de conservare în stare liofilizată. Revista de Proprietate Intelectuală Intelectus. nr.3, 2015, p. 96-104 * |
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| MD1056Y (en) | 2016-07-31 |
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