MA52015A - Digestion séquentielle de polypeptides pour une analyse par spectrométrie de masse - Google Patents
Digestion séquentielle de polypeptides pour une analyse par spectrométrie de masseInfo
- Publication number
- MA52015A MA52015A MA052015A MA52015A MA52015A MA 52015 A MA52015 A MA 52015A MA 052015 A MA052015 A MA 052015A MA 52015 A MA52015 A MA 52015A MA 52015 A MA52015 A MA 52015A
- Authority
- MA
- Morocco
- Prior art keywords
- polypeptides
- analysis
- mass spectrometry
- sequential digestion
- digestion
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title 1
- 230000029087 digestion Effects 0.000 title 1
- 238000004949 mass spectrometry Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21037—Leukocyte elastase (3.4.21.37), i.e. neutrophil-elastase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862642549P | 2018-03-13 | 2018-03-13 |
Publications (1)
Publication Number | Publication Date |
---|---|
MA52015A true MA52015A (fr) | 2021-01-20 |
Family
ID=66182633
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MA052015A MA52015A (fr) | 2018-03-13 | 2019-03-13 | Digestion séquentielle de polypeptides pour une analyse par spectrométrie de masse |
Country Status (12)
Country | Link |
---|---|
US (1) | US20210255075A1 (fr) |
EP (1) | EP3765857A1 (fr) |
JP (1) | JP7411560B2 (fr) |
KR (1) | KR20200131266A (fr) |
CN (1) | CN111868531A (fr) |
AU (1) | AU2019234846A1 (fr) |
CA (1) | CA3093396A1 (fr) |
IL (1) | IL277106B2 (fr) |
MA (1) | MA52015A (fr) |
MX (1) | MX2020009458A (fr) |
SG (1) | SG11202008869UA (fr) |
WO (1) | WO2019178280A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111024863A (zh) * | 2019-12-31 | 2020-04-17 | 上海博威生物医药有限公司 | 一种雷莫芦单抗的肽图的测试方法 |
US20220187197A1 (en) * | 2020-12-16 | 2022-06-16 | Hach Company | Electrochemical digestion |
CN112285247B (zh) * | 2020-12-30 | 2021-06-08 | 南京广祺医药科技有限公司 | 一种定量测定血浆中PEGylated-IL-2(1A)的方法及应用 |
CN114965839A (zh) * | 2022-05-11 | 2022-08-30 | 朗肽生物制药股份有限公司 | 人碱性成纤维细胞生长因子的肽图分析方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SI1425389T1 (sl) | 2001-08-23 | 2012-02-29 | Genmab As | Humana protitelesa, specifična za interlevkin 15 (IL-15) |
JP2004301715A (ja) * | 2003-03-31 | 2004-10-28 | Institute Of Physical & Chemical Research | タンパク質の一次構造情報収集方法、それに用いる疎水性微細粒子担体及びタンパク質一次構造情報自動取得システム |
US20040253659A1 (en) * | 2003-06-11 | 2004-12-16 | Michele Rasamoelisolo | Method for identification of glycosylated polypeptides |
CA2589860A1 (fr) | 2005-01-24 | 2006-08-03 | Amgen Inc. | Anticorps anti-amyloide humanise |
US7592429B2 (en) | 2005-05-03 | 2009-09-22 | Ucb Sa | Sclerostin-binding antibody |
US8003108B2 (en) | 2005-05-03 | 2011-08-23 | Amgen Inc. | Sclerostin epitopes |
US7550259B2 (en) * | 2006-07-12 | 2009-06-23 | Agilent Technologies, Inc. | Linear high-throughput proteomics |
US20090256068A1 (en) * | 2008-04-10 | 2009-10-15 | Konstantinos Petritis | Solution fragmentation systems and processes for proteomics analysis |
KR20100034015A (ko) | 2007-06-20 | 2010-03-31 | 아이알엠 엘엘씨 | 알레르기 질환 치료를 위한 방법 및 조성물 |
US7982016B2 (en) | 2007-09-10 | 2011-07-19 | Amgen Inc. | Antigen binding proteins capable of binding thymic stromal lymphopoietin |
TWI516501B (zh) | 2008-09-12 | 2016-01-11 | 禮納特神經系統科學公司 | Pcsk9拮抗劑類 |
DE102010054580B3 (de) * | 2010-12-15 | 2012-04-26 | Bruker Daltonik Gmbh | Proteomanalyse in Massenspektrometern mit HF-Ionenfallen |
US20130210050A1 (en) * | 2012-02-15 | 2013-08-15 | Northwestern University | Protease for proteomics |
US20210255194A1 (en) * | 2016-02-04 | 2021-08-19 | Oncobiologics, Inc. | Methods for identifying and analyzing amino acid sequences of proteins |
-
2019
- 2019-03-13 WO PCT/US2019/022126 patent/WO2019178280A1/fr unknown
- 2019-03-13 CN CN201980018060.7A patent/CN111868531A/zh active Pending
- 2019-03-13 EP EP19717990.6A patent/EP3765857A1/fr active Pending
- 2019-03-13 CA CA3093396A patent/CA3093396A1/fr active Pending
- 2019-03-13 JP JP2020547033A patent/JP7411560B2/ja active Active
- 2019-03-13 SG SG11202008869UA patent/SG11202008869UA/en unknown
- 2019-03-13 KR KR1020207028464A patent/KR20200131266A/ko unknown
- 2019-03-13 MA MA052015A patent/MA52015A/fr unknown
- 2019-03-13 MX MX2020009458A patent/MX2020009458A/es unknown
- 2019-03-13 US US16/980,344 patent/US20210255075A1/en active Pending
- 2019-03-13 IL IL277106A patent/IL277106B2/en unknown
- 2019-03-13 AU AU2019234846A patent/AU2019234846A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA3093396A1 (fr) | 2019-09-19 |
KR20200131266A (ko) | 2020-11-23 |
IL277106A (en) | 2020-10-29 |
IL277106B2 (en) | 2024-03-01 |
US20210255075A1 (en) | 2021-08-19 |
SG11202008869UA (en) | 2020-10-29 |
EP3765857A1 (fr) | 2021-01-20 |
IL277106B1 (en) | 2023-11-01 |
JP7411560B2 (ja) | 2024-01-11 |
AU2019234846A1 (en) | 2020-09-24 |
CN111868531A (zh) | 2020-10-30 |
MX2020009458A (es) | 2020-10-12 |
WO2019178280A1 (fr) | 2019-09-19 |
JP2021515893A (ja) | 2021-06-24 |
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