SG11202008869UA - Sequential digestion of polypeptides for mass spectrometric analysis - Google Patents
Sequential digestion of polypeptides for mass spectrometric analysisInfo
- Publication number
- SG11202008869UA SG11202008869UA SG11202008869UA SG11202008869UA SG11202008869UA SG 11202008869U A SG11202008869U A SG 11202008869UA SG 11202008869U A SG11202008869U A SG 11202008869UA SG 11202008869U A SG11202008869U A SG 11202008869UA SG 11202008869U A SG11202008869U A SG 11202008869UA
- Authority
- SG
- Singapore
- Prior art keywords
- polypeptides
- mass spectrometric
- spectrometric analysis
- sequential digestion
- digestion
- Prior art date
Links
- 230000029087 digestion Effects 0.000 title 1
- 238000004949 mass spectrometry Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21037—Leukocyte elastase (3.4.21.37), i.e. neutrophil-elastase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
- G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201862642549P | 2018-03-13 | 2018-03-13 | |
PCT/US2019/022126 WO2019178280A1 (fr) | 2018-03-13 | 2019-03-13 | Digestion séquentielle de polypeptides pour une analyse par spectrométrie de masse |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11202008869UA true SG11202008869UA (en) | 2020-10-29 |
Family
ID=66182633
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202008869UA SG11202008869UA (en) | 2018-03-13 | 2019-03-13 | Sequential digestion of polypeptides for mass spectrometric analysis |
Country Status (12)
Country | Link |
---|---|
US (1) | US20210255075A1 (fr) |
EP (1) | EP3765857A1 (fr) |
JP (1) | JP7411560B2 (fr) |
KR (1) | KR20200131266A (fr) |
CN (1) | CN111868531A (fr) |
AU (1) | AU2019234846A1 (fr) |
CA (1) | CA3093396A1 (fr) |
IL (1) | IL277106B2 (fr) |
MA (1) | MA52015A (fr) |
MX (1) | MX2020009458A (fr) |
SG (1) | SG11202008869UA (fr) |
WO (1) | WO2019178280A1 (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111024863A (zh) * | 2019-12-31 | 2020-04-17 | 上海博威生物医药有限公司 | 一种雷莫芦单抗的肽图的测试方法 |
EP4263443A1 (fr) * | 2020-12-16 | 2023-10-25 | Hach Company | Digestion électrochimique |
CN112285247B (zh) * | 2020-12-30 | 2021-06-08 | 南京广祺医药科技有限公司 | 一种定量测定血浆中PEGylated-IL-2(1A)的方法及应用 |
CN114965839A (zh) * | 2022-05-11 | 2022-08-30 | 朗肽生物制药股份有限公司 | 人碱性成纤维细胞生长因子的肽图分析方法 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1425389B1 (fr) | 2001-08-23 | 2011-11-02 | Genmab A/S | Anticorps humains specifiques diriges contre l'interleukine 15 (il-15) |
JP2004301715A (ja) * | 2003-03-31 | 2004-10-28 | Institute Of Physical & Chemical Research | タンパク質の一次構造情報収集方法、それに用いる疎水性微細粒子担体及びタンパク質一次構造情報自動取得システム |
US20040253659A1 (en) * | 2003-06-11 | 2004-12-16 | Michele Rasamoelisolo | Method for identification of glycosylated polypeptides |
EP1841455A1 (fr) | 2005-01-24 | 2007-10-10 | Amgen Inc. | Anticorps anti-amyloide humanise |
US7592429B2 (en) | 2005-05-03 | 2009-09-22 | Ucb Sa | Sclerostin-binding antibody |
US8003108B2 (en) | 2005-05-03 | 2011-08-23 | Amgen Inc. | Sclerostin epitopes |
US7550259B2 (en) * | 2006-07-12 | 2009-06-23 | Agilent Technologies, Inc. | Linear high-throughput proteomics |
US20090256068A1 (en) * | 2008-04-10 | 2009-10-15 | Konstantinos Petritis | Solution fragmentation systems and processes for proteomics analysis |
EA201000006A1 (ru) | 2007-06-20 | 2010-10-29 | Айрм Ллк | Способы и композиции для лечения аллергических заболеваний |
US7982016B2 (en) | 2007-09-10 | 2011-07-19 | Amgen Inc. | Antigen binding proteins capable of binding thymic stromal lymphopoietin |
TWI516501B (zh) | 2008-09-12 | 2016-01-11 | 禮納特神經系統科學公司 | Pcsk9拮抗劑類 |
DE102010054580B3 (de) * | 2010-12-15 | 2012-04-26 | Bruker Daltonik Gmbh | Proteomanalyse in Massenspektrometern mit HF-Ionenfallen |
US20130210050A1 (en) * | 2012-02-15 | 2013-08-15 | Northwestern University | Protease for proteomics |
MX2018009339A (es) * | 2016-02-04 | 2019-01-10 | Oncobiologics Inc | Métodos para la identificación y análisis de secuencias de aminoácidos de las proteínas. |
-
2019
- 2019-03-13 KR KR1020207028464A patent/KR20200131266A/ko unknown
- 2019-03-13 MX MX2020009458A patent/MX2020009458A/es unknown
- 2019-03-13 EP EP19717990.6A patent/EP3765857A1/fr active Pending
- 2019-03-13 SG SG11202008869UA patent/SG11202008869UA/en unknown
- 2019-03-13 WO PCT/US2019/022126 patent/WO2019178280A1/fr unknown
- 2019-03-13 CN CN201980018060.7A patent/CN111868531A/zh active Pending
- 2019-03-13 US US16/980,344 patent/US20210255075A1/en active Pending
- 2019-03-13 MA MA052015A patent/MA52015A/fr unknown
- 2019-03-13 JP JP2020547033A patent/JP7411560B2/ja active Active
- 2019-03-13 CA CA3093396A patent/CA3093396A1/fr active Pending
- 2019-03-13 AU AU2019234846A patent/AU2019234846A1/en active Pending
- 2019-03-13 IL IL277106A patent/IL277106B2/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP7411560B2 (ja) | 2024-01-11 |
WO2019178280A1 (fr) | 2019-09-19 |
MX2020009458A (es) | 2020-10-12 |
US20210255075A1 (en) | 2021-08-19 |
CN111868531A (zh) | 2020-10-30 |
IL277106A (en) | 2020-10-29 |
CA3093396A1 (fr) | 2019-09-19 |
IL277106B2 (en) | 2024-03-01 |
KR20200131266A (ko) | 2020-11-23 |
EP3765857A1 (fr) | 2021-01-20 |
IL277106B1 (en) | 2023-11-01 |
MA52015A (fr) | 2021-01-20 |
AU2019234846A1 (en) | 2020-09-24 |
JP2021515893A (ja) | 2021-06-24 |
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