MA49352A - Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome - Google Patents

Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome

Info

Publication number
MA49352A
MA49352A MA049352A MA49352A MA49352A MA 49352 A MA49352 A MA 49352A MA 049352 A MA049352 A MA 049352A MA 49352 A MA49352 A MA 49352A MA 49352 A MA49352 A MA 49352A
Authority
MA
Morocco
Prior art keywords
high yield
transcriptome analysis
polynucleotide library
library sequencing
yield polynucleotide
Prior art date
Application number
MA049352A
Other languages
English (en)
French (fr)
Inventor
Adrian Wrangham Briggs
Rajagopal Chari
Stephen Jacob Goldfless
Ronald Hause
Yue Jiang
François Vigneault
Original Assignee
Abvitro Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abvitro Llc filed Critical Abvitro Llc
Publication of MA49352A publication Critical patent/MA49352A/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
MA049352A 2017-05-26 2018-05-25 Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome MA49352A (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762511949P 2017-05-26 2017-05-26

Publications (1)

Publication Number Publication Date
MA49352A true MA49352A (fr) 2020-04-08

Family

ID=62621068

Family Applications (1)

Application Number Title Priority Date Filing Date
MA049352A MA49352A (fr) 2017-05-26 2018-05-25 Séquençage de bibliothèque de polynucléotides à haut rendement et analyse de transcriptome

Country Status (10)

Country Link
US (1) US12049667B2 (cg-RX-API-DMAC10.html)
EP (1) EP3631012B1 (cg-RX-API-DMAC10.html)
JP (1) JP7308187B2 (cg-RX-API-DMAC10.html)
KR (1) KR102550778B1 (cg-RX-API-DMAC10.html)
CN (1) CN111148849A (cg-RX-API-DMAC10.html)
AU (1) AU2018273999A1 (cg-RX-API-DMAC10.html)
CA (1) CA3064205A1 (cg-RX-API-DMAC10.html)
ES (1) ES2926722T3 (cg-RX-API-DMAC10.html)
MA (1) MA49352A (cg-RX-API-DMAC10.html)
WO (1) WO2018218222A1 (cg-RX-API-DMAC10.html)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
US12049667B2 (en) 2017-05-26 2024-07-30 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
CA3124887A1 (en) * 2018-12-28 2020-07-02 National University Of Singapore Methods for targeted complementary dna enrichment
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
JP2022543278A (ja) * 2019-08-05 2022-10-11 ミッション バイオ インコーポレイテッド 細胞軌道を測定するためのシングルセル分析のための方法及び装置
WO2021072075A1 (en) * 2019-10-09 2021-04-15 Edward Fritsch Multi-domain protein vaccine
EP4055160B1 (en) * 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021168015A1 (en) * 2020-02-17 2021-08-26 Universal Sequencing Technology Corporation Methods of barcoding nucleic acid for detection and sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021231779A1 (en) * 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
AU2021275128A1 (en) * 2020-05-20 2023-01-19 Element Biosciences, Inc. Methods for paired-end sequencing library preparation
US20230203600A1 (en) * 2020-05-29 2023-06-29 Riken Method for treating cell population and method for analyzing genes included in cell population
CN115803445A (zh) * 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
KR102468839B1 (ko) 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법
CN111549025B (zh) * 2020-06-08 2021-09-14 郑州大学第一附属医院 链置换引物和细胞转录组文库构建方法
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CA3189006A1 (en) * 2020-09-07 2022-03-10 Matthew Asuka KUBIT Methods of assaying a biological cell
JP2023547732A (ja) * 2020-11-03 2023-11-13 エイシーティ・ジェノミックス(アイピー)リミテッド 遺伝子改変を検出するための標的化シークエンシング方法およびそのキット
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12378596B2 (en) * 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
US11111489B1 (en) 2021-01-06 2021-09-07 Think Therapeutics, Inc. Multiplexed testing of lymphocytes for antigen specificity
CN113512767B (zh) * 2021-04-30 2022-09-23 天津诺禾致源生物信息科技有限公司 用于构建small RNA文库的接头、试剂盒及small RNA文库的构建方法
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN113462750B (zh) * 2021-06-29 2024-08-27 浙江峻山生物科技有限公司 一种提高病毒核酸检测灵敏度的样本保存液及其制备方法
WO2023004358A1 (en) * 2021-07-20 2023-01-26 Donald Danforth Plant Science Center All-in-one rna sequencing assay and uses thereof
WO2023086819A1 (en) * 2021-11-09 2023-05-19 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using randomer reverse primers
WO2023141604A2 (en) * 2022-01-21 2023-07-27 Mission Bio, Inc. Methods of molecular tagging for single-cell analysis
CN116010022B (zh) * 2022-11-29 2026-03-17 兴业银行股份有限公司 Presto自动化伸缩的系统、方法及介质
CN116377024A (zh) * 2023-02-20 2023-07-04 迈恒生物技术(上海)有限公司 一种同时测单b细胞抗体基因和转录组的高通量测序方法
WO2025019313A1 (en) * 2023-07-14 2025-01-23 The Broad Institute, Inc. Method for assembling gene libraries from a pool of oligonucleotides
CN118280446B (zh) * 2024-05-31 2024-08-13 浙江大学 植物单细胞非编码基因鉴定和功能预测方法、装置及应用
CN118727162B (zh) * 2024-06-05 2025-04-04 首都医科大学附属北京口腔医院 单细胞4c文库构建方法和检测方法
WO2026058704A1 (ja) * 2024-09-10 2026-03-19 国立大学法人 東京大学 Dnaバーコード配列を含む一本鎖 dnaの合成方法(method for synthesizing single-stranded dna containing dna barcode sequences)
CN120400136A (zh) * 2025-07-01 2025-08-01 杭州华大生命科学研究院 模板转换寡核苷酸、试剂盒及其应用

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4766067A (en) 1985-05-31 1988-08-23 President And Fellows Of Harvard College Gene amplification
GB8601597D0 (en) 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4795699A (en) 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4921794A (en) 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5168038A (en) 1988-06-17 1992-12-01 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5142033A (en) 1988-09-23 1992-08-25 Hoffmann-La Roche Inc. Structure-independent DNA amplification by the polymerase chain reaction
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US4994370A (en) 1989-01-03 1991-02-19 The United States Of America As Represented By The Department Of Health And Human Services DNA amplification technique
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69233528T2 (de) 1991-11-25 2006-03-16 Enzon, Inc. Verfahren zur Herstellung von multivalenten antigenbindenden Proteinen
JPH07503132A (ja) 1991-12-17 1995-04-06 ジェンファーム インターナショナル,インコーポレイティド 異種抗体を産生することができるトランスジェニック非ヒト動物
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AU730633B2 (en) 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
EP1068356B8 (en) 1998-04-03 2007-01-03 Adnexus Therapeutics, Inc. Addressable protein arrays
JP3813818B2 (ja) 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ オリゴヌクレオチドおよびdna分子のヌクレオチド配列の決定方法
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB9928787D0 (en) 1999-12-03 2000-02-02 Medical Res Council Direct screening method
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
PT1421115E (pt) 2001-08-31 2005-07-29 Avidex Ltd Receptor de celulas t soluvel
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
AU2003271904B2 (en) 2002-10-09 2009-03-05 Adaptimmune Limited Single chain recombinant T cell receptors
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7643818B2 (en) 2004-11-22 2010-01-05 Seven Networks, Inc. E-mail messaging to/from a mobile terminal
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
EP3150727B1 (en) 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
GB2502409B8 (en) 2008-09-23 2014-10-15 Bio Rad Laboratories Droplet-based assay system
US20120010091A1 (en) 2009-03-30 2012-01-12 Illumina, Inc. Gene expression analysis in single cells
WO2011044186A1 (en) 2009-10-06 2011-04-14 The Board Of Trustees Of The University Of Illinois Human single-chain t cell receptors
US9902950B2 (en) 2010-10-08 2018-02-27 President And Fellows Of Harvard College High-throughput single cell barcoding
EP3561073A1 (en) 2010-10-08 2019-10-30 President and Fellows of Harvard College High-throughput immune sequencing
WO2013033271A2 (en) 2011-08-29 2013-03-07 Derren Barken Method to augment immune system in response to disease or injury
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
RU2578009C2 (ru) 2013-05-08 2016-03-20 Закрытое акционерное общество "ЕВРОГЕН" Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках
KR102366116B1 (ko) * 2013-06-27 2022-02-23 10엑스 제노믹스, 인크. 샘플 처리를 위한 조성물 및 방법
GB201317301D0 (en) * 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
US10208338B2 (en) * 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
EP3134536B1 (en) 2014-04-21 2019-12-18 President and Fellows of Harvard College Systems and methods for barcoding nucleic acids
EP4574974A3 (en) * 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
JP2017532024A (ja) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド コンポジット単一細胞核酸分析のための液滴ベースの方法および機器
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
EP3234130B1 (en) * 2014-12-19 2020-11-25 The Broad Institute, Inc. Methods for profiling the t-cell- receptor repertoire
CN107530654A (zh) * 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN107614684A (zh) * 2015-04-17 2018-01-19 哈佛学院院长及董事 用于基因测序和其它应用的条形编码系统及方法
CN107849560B (zh) 2015-04-27 2021-10-22 阿布维特罗有限责任公司 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
KR20180085717A (ko) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용
US20180274021A1 (en) 2015-09-24 2018-09-27 Abvitro Llc Single amplicon activated exclusion pcr
ES2768957T3 (es) 2015-09-24 2020-06-24 Abvitro Llc Composiciones de anticuerpos contra el VIH y métodos de uso
AU2016326734B2 (en) 2015-09-25 2022-07-07 Abvitro Llc High throughput process for T cell receptor target identification of natively-paired T cell receptor sequences
AU2017332495A1 (en) 2016-09-24 2019-04-11 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2018083849A1 (ja) 2016-11-02 2018-05-11 三菱電機株式会社 水処理装置及び水処理方法
PL3618842T3 (pl) 2017-05-01 2024-02-19 Juno Therapeutics, Inc. Kombinacja terapii komórkowej i związku immunomodulującego
US12049667B2 (en) 2017-05-26 2024-07-30 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
EP4516896A3 (en) 2017-08-09 2025-05-14 Juno Therapeutics, Inc. Methods and compositions for preparing genetically engineered cells
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
MA50079A (fr) 2017-09-07 2020-07-15 Juno Therapeutics Inc Procédés d'identification de caractéristiques cellulaires relatives à des réponses associées à une thérapie cellulaire
US11851679B2 (en) 2017-11-01 2023-12-26 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors

Also Published As

Publication number Publication date
RU2019143662A3 (cg-RX-API-DMAC10.html) 2022-02-25
KR20200039623A (ko) 2020-04-16
US20200354784A1 (en) 2020-11-12
CN111148849A (zh) 2020-05-12
EP3631012A1 (en) 2020-04-08
CA3064205A1 (en) 2018-11-29
EP3631012B1 (en) 2022-06-08
RU2019143662A (ru) 2021-06-28
ES2926722T3 (es) 2022-10-27
AU2018273999A1 (en) 2019-12-05
US12049667B2 (en) 2024-07-30
KR102550778B1 (ko) 2023-07-03
JP2020523034A (ja) 2020-08-06
WO2018218222A1 (en) 2018-11-29
JP7308187B2 (ja) 2023-07-13

Similar Documents

Publication Publication Date Title
IL283416A (en) Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis
IL272234A (en) High-throughput single-cell transcriptome libraries and methods of making and of using
EP3445876C0 (en) ANALYSIS OF ACCESSIBLE CHROMATIN BY A SINGLE CELL TRANSPOSASE
EP3323070A4 (en) Neo-antigen-ANALYSIS
DK3171765T3 (da) Indsamling og analyse af data til diagnostiske formål
EP3449438A4 (en) Customer experience analytics
DK3896171T5 (da) Fremgangsmåde for behandling af polynukleotider til analyse af individuelle celler
EP4282977C0 (en) ANALYSIS OF MULTIPLE SPATIALLY DIFFERENTIATED NUCLEIC ACIDS FROM ORGANIC SPECIMENS
MA42847A (fr) Analyse de micro-moment
DK3429543T3 (da) Selektivt ventilerede biologiske analyseanordninger og tilhørende fremgangsmåder
DK4071469T3 (da) Fremgangsmåder til analyse af kromatografidata
EP3623462A4 (en) GENE SEQUENCING CHIP AND GENE SEQUENCING PROCEDURES
DK3256605T3 (da) Påvisning af mutationer til cancerscreening og føtal analyse
DK3967775T3 (da) Analyse af fragmenteringsmønstre af cellefrit DNA
DK3332034T3 (da) Systemer og fremgangsmåder til genomisk analyse
EP4112738C0 (en) MULTIGENE ANALYSIS OF TUMOR SAMPLES
DK3302804T3 (da) Prøveholder og analysesystem til udførelse af specifikke reaktioner
DK3792378T3 (da) Fremgangsmåder og anordninger til analyse af partikler
DK3350342T3 (da) Sondesæt til analyse af en DNA-prøve og fremgangsmåde til anvendelse deraf
EP3439547A4 (en) METHOD FOR ANALYZING DIGITAL DATA
EP3358346A4 (en) PROCESS FOR ENANTIOMER ANALYSIS
EP3645213A4 (en) DRIVER TOOL AND PROCEDURE
FR3026745B1 (fr) Methode d'analyse et interpretation automatisee d'un antibiogramme
EP3353536A4 (en) ANALYSIS OF ELEMENTS THROUGH SOLUTION CATHODE GLIMETRY LOAD
EP3497241A4 (en) GENOME SEQUENCING WITH EXTREMELY LOW COVERAGE AND USES THEREOF