LV10119B - Method of preparing lysocyme dimers - Google Patents
Method of preparing lysocyme dimers Download PDFInfo
- Publication number
- LV10119B LV10119B LVP-92-506A LV920506A LV10119B LV 10119 B LV10119 B LV 10119B LV 920506 A LV920506 A LV 920506A LV 10119 B LV10119 B LV 10119B
- Authority
- LV
- Latvia
- Prior art keywords
- lysozyme
- solution
- elution
- elution step
- fractions
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Communicable Diseases (AREA)
- Zoology (AREA)
- Oncology (AREA)
- Wood Science & Technology (AREA)
- Rheumatology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Pain & Pain Management (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Claims (9)
- 5 10 15 20 25 LIZOCĪMA DIMĒRA IEGŪŠANAS METODE LV 10119 PATENTA FORMULA 1. Metode attīrīta lizocīma dimēra iegūšanai ar sekojošām stadijām: a) lizocīma šķīduma pagatavošana, pievienojot lizocīma monomēru bufera šķīdumam, labāk dinātrija hidrogenfosfata dihidrāta bufera šķīdumam, noregulējot pH vismaz apmēram 9, labāk apmēram 10, pievienojot, piemēram, NaOH; b) suberimidāta, labāk dimetil-suberimidāta, kā savienojoša līdzekļa pievienošana, lai dimerizētu lizocīma monomēru lizocīma šķīdumā, uzturot pH vismaz vienādu ar apmēram 9, labāk apmēram 10; c) dimerizācijas reakcijas pārtraukšana dotā brīdī, pazeminot pH līdz aptuveni 7; d) dimerizētā lizocīma šķīduma attīrīšana, veicot eluēšanas pirmo stadiju, kurā šķīdumu laiž caur jonu apmaiņas kolonu un savāc frakcijas , kas sastāv galvenokārt no lizocīma dimērās formas; e) pirmajā eluēšanas stadijā savākto frakciju filtrēšana otrreizējas eluēšanas ceļā, kurā frakcijas, kas savāktas pēc pirmās attīrīšanas stadijas, no jauna laiž caur jonu apmaiņas kolonu; un f) otrajā eluēšanas stadijā iegūtā augsti attīrītā lizocīma dimēra produkta savākšana. 2. Metode, saskaņā ar 1. punktu, kur dimerizācijas stadijas veic vismaz kādos no sekojošiem apstākļiem: - pie istabas temperatūras; - šķīdums tiek nepārtraukti maisīts; * dimerizācijas reakciju pārtrauc, pievienojot aģentu, kas izvēlēts no grupas, kurā ietilpst HC1 un amonija acetāta šķīdums, vai ir to abu kombinācija. 30 2
- 3. Metode, saskaņā ar 1. vai 2. punktu, kur turpmāk paredzēta pēc dimerizācijas neizšķīdušo daļiņu filtrēšana ar poraina filtra palīdzību, kura poru izmērs ir labāk apmēram 0.4 -0.5 μπι.
- 4. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, kur turpmāk paredzēta nedimerizētā lizocīma monomēra savākšana pēc dimerizācijas un nedimerizēto monomēru atgriešana dimerizācijas procesa atkārtotā ciklā.
- 5. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, 10 kur lizocīma monomēra šķīdums satur 40 - 60 g lizocīma monomēra un 4 - 6 1 bufera, un kuram pievieno sašūšanas reaģentu daudzumā apmēram 4 - 6 g.
- 6. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, kur frakcijas pēc eluēšanas pirmās stadijas un/vai frakcijas, kas 15 savāktas pēc otrās eluēšanas stadijas, analizē uz lizocīma saturu ar gēla elektroforēzes palīdzību.
- 7. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, kur frakcijas, kas savāktas pēc pirmās eluēšanas stadijas un/vai frakcijas, kas savāktas pēc otrās eluēšanas stadijas, koncentrē ar 20 tangenciālas caurteces sistēmas palīdzību.
- 8. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, kur pirmo eluēšanas stadiju veic, izmantojot kā jonu apmaiņas sveķus sašūtu dekstrānu.
- 9. Metode, saskaņā ar jebkuru no iepriekšējiem punktiem, 25 kur otro eluēšanas stadiju veic, izmantojot kā jonu apmaiņas sveķus sašūtu dekstrānu.
- 10. Metode saskaņā ar jebkuru no iepriekšējiem punktiem, kur attīrīto lizocīma dimēra produktu, kas savākts pēc otrās eluēšanas stadijas, liofilizē. 30
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1990/000140 WO1991010731A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| CA002073351A CA2073351C (en) | 1990-01-08 | 1990-01-08 | Lysozyme dimers and method of preparing them |
| HU9202250A HU214509B (hu) | 1990-01-08 | 1990-01-08 | Eljárás tisztított lizozim dimerek előállítására |
| SG1996007945A SG49871A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| RO92-0931A RO114805B1 (ro) | 1990-01-08 | 1990-01-08 | Dimer de lizozim purificat si procedeu de obtinere a acestuia |
| OA60242A OA09706A (en) | 1990-01-08 | 1992-07-07 | Method of preparing lysozyme dimers. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LV10119A LV10119A (lv) | 1994-05-10 |
| LV10119B true LV10119B (en) | 1995-02-20 |
Family
ID=33136306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LVP-92-506A LV10119B (en) | 1990-01-08 | 1992-12-28 | Method of preparing lysocyme dimers |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP0509984B1 (lv) |
| JP (1) | JP2732514B2 (lv) |
| KR (1) | KR0158216B1 (lv) |
| AU (1) | AU654651B2 (lv) |
| BG (1) | BG61040B1 (lv) |
| BR (1) | BR9007971A (lv) |
| CA (1) | CA2073351C (lv) |
| DE (1) | DE69032375T2 (lv) |
| DK (1) | DK0509984T3 (lv) |
| ES (1) | ES2118719T3 (lv) |
| FI (1) | FI101399B1 (lv) |
| HK (1) | HK1007456A1 (lv) |
| HU (1) | HU214509B (lv) |
| LT (1) | LT3422B (lv) |
| LV (1) | LV10119B (lv) |
| MC (2) | MC2242A1 (lv) |
| NO (1) | NO302484B1 (lv) |
| OA (1) | OA09706A (lv) |
| RO (1) | RO114805B1 (lv) |
| SG (1) | SG49871A1 (lv) |
| WO (1) | WO1991010731A1 (lv) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL173978B1 (pl) * | 1992-07-13 | 1998-05-29 | Nika Health Products Ltd | Sposób oddziaływania na wydzielanie cytokin w hodowli limfocytów krwi obwodowej |
| US6183742B1 (en) | 1992-07-13 | 2001-02-06 | Nika Health Products, Limited | Applications of lysozyme dimer |
| DZ1964A1 (fr) * | 1995-01-13 | 2002-10-15 | Nika Health Products Ltd | Nouvelle applications d'un dimère de lysozyme. |
| US6123937A (en) * | 1997-03-14 | 2000-09-26 | Nika Health Products, Limited | Applications of lysozyme dimer |
| US7348301B2 (en) * | 2006-02-16 | 2008-03-25 | Buckman Laboratories International, Inc. | Lysozyme-based method and composition to control the growth of microorganisms in aqueous systems |
| CN108424464A (zh) * | 2011-10-05 | 2018-08-21 | 洛克菲勒大学 | 二聚噬菌体溶素 |
| CN111751468A (zh) * | 2020-07-03 | 2020-10-09 | 上海艾魁英生物科技有限公司 | 溶菌酶二聚体的纯化及其标准品制备的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7850731B2 (en) | 2006-10-04 | 2010-12-14 | Seaspine, Inc. | Articulating spinal implant |
-
1990
- 1990-01-08 MC MC90US9000140D patent/MC2242A1/xx unknown
- 1990-01-08 EP EP90902033A patent/EP0509984B1/en not_active Expired - Lifetime
- 1990-01-08 WO PCT/US1990/000140 patent/WO1991010731A1/en active IP Right Grant
- 1990-01-08 DK DK90902033T patent/DK0509984T3/da active
- 1990-01-08 JP JP2502143A patent/JP2732514B2/ja not_active Expired - Fee Related
- 1990-01-08 SG SG1996007945A patent/SG49871A1/en unknown
- 1990-01-08 KR KR1019920701566A patent/KR0158216B1/ko not_active IP Right Cessation
- 1990-01-08 MC MC90US9000140D patent/MC2243A1/xx unknown
- 1990-01-08 HU HU9202250A patent/HU214509B/hu unknown
- 1990-01-08 BR BR909007971A patent/BR9007971A/pt not_active Application Discontinuation
- 1990-01-08 DE DE69032375T patent/DE69032375T2/de not_active Expired - Fee Related
- 1990-01-08 ES ES90902033T patent/ES2118719T3/es not_active Expired - Lifetime
- 1990-01-08 CA CA002073351A patent/CA2073351C/en not_active Expired - Lifetime
- 1990-01-08 AU AU48474/90A patent/AU654651B2/en not_active Ceased
- 1990-01-08 RO RO92-0931A patent/RO114805B1/ro unknown
-
1992
- 1992-07-03 NO NO922626A patent/NO302484B1/no not_active IP Right Cessation
- 1992-07-07 FI FI923123A patent/FI101399B1/fi active
- 1992-07-07 BG BG96578A patent/BG61040B1/bg unknown
- 1992-07-07 OA OA60242A patent/OA09706A/en unknown
- 1992-12-28 LV LVP-92-506A patent/LV10119B/en unknown
-
1993
- 1993-06-02 LT LTIP594A patent/LT3422B/lt not_active IP Right Cessation
-
1998
- 1998-06-25 HK HK98106623A patent/HK1007456A1/xx not_active IP Right Cessation
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