LU81699A1 - Gels d'agarose conditionnee dans des tubes metalliques - Google Patents
Gels d'agarose conditionnee dans des tubes metalliques Download PDFInfo
- Publication number
- LU81699A1 LU81699A1 LU81699A LU81699A LU81699A1 LU 81699 A1 LU81699 A1 LU 81699A1 LU 81699 A LU81699 A LU 81699A LU 81699 A LU81699 A LU 81699A LU 81699 A1 LU81699 A1 LU 81699A1
- Authority
- LU
- Luxembourg
- Prior art keywords
- weight
- aqueous gels
- aqueous
- gels according
- gel
- Prior art date
Links
- 239000000499 gel Substances 0.000 title claims description 92
- 229910052751 metal Inorganic materials 0.000 title claims description 9
- 239000002184 metal Substances 0.000 title claims description 9
- 230000001143 conditioned effect Effects 0.000 title 1
- 229920000936 Agarose Polymers 0.000 claims description 30
- 229920001817 Agar Polymers 0.000 claims description 20
- 235000010419 agar Nutrition 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000872 buffer Substances 0.000 claims description 18
- 241000206672 Gelidium Species 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 16
- 238000001962 electrophoresis Methods 0.000 claims description 10
- 238000000760 immunoelectrophoresis Methods 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 9
- 235000018102 proteins Nutrition 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 8
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 239000000600 sorbitol Substances 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 125000000524 functional group Chemical group 0.000 claims description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 4
- 229930195725 Mannitol Natural products 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 239000000594 mannitol Substances 0.000 claims description 4
- 235000010355 mannitol Nutrition 0.000 claims description 4
- 125000003277 amino group Chemical group 0.000 claims description 3
- 238000001155 isoelectric focusing Methods 0.000 claims description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- 229920003169 water-soluble polymer Polymers 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 230000002378 acidificating effect Effects 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims description 2
- 125000001931 aliphatic group Chemical group 0.000 claims description 2
- 239000005018 casein Substances 0.000 claims description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 2
- 235000021240 caseins Nutrition 0.000 claims description 2
- 238000009792 diffusion process Methods 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 230000000951 immunodiffusion Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- -1 alicyclic carboxylic acid Chemical class 0.000 claims 1
- 239000000022 bacteriostatic agent Substances 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 239000000243 solution Substances 0.000 description 28
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 15
- 229960002319 barbital Drugs 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- 239000007983 Tris buffer Substances 0.000 description 7
- 235000010356 sorbitol Nutrition 0.000 description 7
- 229960002920 sorbitol Drugs 0.000 description 7
- 238000009835 boiling Methods 0.000 description 6
- 239000000470 constituent Substances 0.000 description 6
- 239000011543 agarose gel Substances 0.000 description 5
- 229920002401 polyacrylamide Polymers 0.000 description 5
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 4
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000001879 gelation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000036571 hydration Effects 0.000 description 3
- 238000006703 hydration reaction Methods 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 239000012954 diazonium Substances 0.000 description 2
- 150000001989 diazonium salts Chemical class 0.000 description 2
- 229960001031 glucose Drugs 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000010258 microfractionation Methods 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 239000003505 polymerization initiator Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 159000000000 sodium salts Chemical class 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 239000002966 varnish Substances 0.000 description 2
- AVYGCQXNNJPXSS-UHFFFAOYSA-N 2,5-dichloroaniline Chemical compound NC1=CC(Cl)=CC=C1Cl AVYGCQXNNJPXSS-UHFFFAOYSA-N 0.000 description 1
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical class NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 125000002723 alicyclic group Chemical group 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000007982 barbital buffer Substances 0.000 description 1
- 229960000796 barbital sodium Drugs 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- ZVSKZLHKADLHSD-UHFFFAOYSA-N benzanilide Chemical compound C=1C=CC=CC=1C(=O)NC1=CC=CC=C1 ZVSKZLHKADLHSD-UHFFFAOYSA-N 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229960003260 chlorhexidine Drugs 0.000 description 1
- OSASVXMJTNOKOY-UHFFFAOYSA-N chlorobutanol Chemical compound CC(C)(O)C(Cl)(Cl)Cl OSASVXMJTNOKOY-UHFFFAOYSA-N 0.000 description 1
- 229960004926 chlorobutanol Drugs 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 235000014510 cooky Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- QMMMCTXNYMSXLI-UHFFFAOYSA-N fast blue B Chemical compound C1=C([N+]#N)C(OC)=CC(C=2C=C(OC)C([N+]#N)=CC=2)=C1 QMMMCTXNYMSXLI-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N hexane Substances CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- IZXGZAJMDLJLMF-UHFFFAOYSA-N methylaminomethanol Chemical compound CNCO IZXGZAJMDLJLMF-UHFFFAOYSA-N 0.000 description 1
- CNXZLZNEIYFZGU-UHFFFAOYSA-N n-(4-amino-2,5-diethoxyphenyl)benzamide Chemical compound C1=C(N)C(OCC)=CC(NC(=O)C=2C=CC=CC=2)=C1OCC CNXZLZNEIYFZGU-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 229920003170 water-soluble synthetic polymer Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Polymers & Plastics (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Colloid Chemistry (AREA)
- Medicinal Preparation (AREA)
- Jellies, Jams, And Syrups (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7827179 | 1978-09-22 | ||
| FR7827179A FR2436791A1 (fr) | 1978-09-22 | 1978-09-22 | Gels d'agarose conditionnes dans des tubes metalliques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LU81699A1 true LU81699A1 (fr) | 1981-04-17 |
Family
ID=9212916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LU81699A LU81699A1 (fr) | 1978-09-22 | 1979-09-19 | Gels d'agarose conditionnee dans des tubes metalliques |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0009430B1 (pt) |
| JP (1) | JPS5544396A (pt) |
| AT (1) | ATA615579A (pt) |
| BE (1) | BE878846A (pt) |
| CA (1) | CA1143681A (pt) |
| CH (1) | CH643575A5 (pt) |
| DE (1) | DE2962292D1 (pt) |
| FR (1) | FR2436791A1 (pt) |
| IT (1) | IT1119166B (pt) |
| LU (1) | LU81699A1 (pt) |
| PT (1) | PT70194A (pt) |
| SE (1) | SE7907857L (pt) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59126236A (ja) * | 1983-01-08 | 1984-07-20 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| GB8428876D0 (en) * | 1984-11-15 | 1984-12-27 | Ciba Geigy Ag | Diagnostic strips |
| GB8514052D0 (en) * | 1985-06-04 | 1985-07-10 | Geistlich Soehne Ag | Compositions |
| CH666907A5 (fr) * | 1985-11-13 | 1988-08-31 | Medinational Harley Street Lim | Procede de preparation d'un complexe de polygalactane macromoleculaire et de compose azote, et utilisation de ce complexe. |
| DE3737815A1 (de) * | 1987-11-06 | 1989-05-18 | Wacker Chemitronic | Siliciumscheiben zur erzeugung von oxidschichten hoher durchschlagsfestigkeit und verfahren zur ihrer herstellung |
| US5068019A (en) * | 1989-05-19 | 1991-11-26 | Fuji Photo Film Co., Ltd. | Electrophoresis film support |
| DE3935020A1 (de) * | 1989-10-20 | 1991-04-25 | Serva Feinbiochem Gmbh & Co | Verbesserte elektrophoresegele aus agarose |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE26199E (en) * | 1967-05-09 | Agar preparation and process of producing the same | ||
| US2963373A (en) * | 1958-11-17 | 1960-12-06 | American Molasses Company | Icings and method of preparing them and cold-water soluble agar and carrageen gums for said icings |
| BE666282A (pt) * | 1966-04-18 | |||
| US3766047A (en) * | 1972-09-11 | 1973-10-16 | F Elevitch | Gel for electrophoresis |
-
1978
- 1978-09-22 FR FR7827179A patent/FR2436791A1/fr active Granted
-
1979
- 1979-09-04 DE DE7979400607T patent/DE2962292D1/de not_active Expired
- 1979-09-04 EP EP79400607A patent/EP0009430B1/fr not_active Expired
- 1979-09-18 BE BE0/197191A patent/BE878846A/fr not_active IP Right Cessation
- 1979-09-18 PT PT70194A patent/PT70194A/pt unknown
- 1979-09-19 AT AT0615579A patent/ATA615579A/de not_active Application Discontinuation
- 1979-09-19 LU LU81699A patent/LU81699A1/fr unknown
- 1979-09-20 IT IT68841/79A patent/IT1119166B/it active
- 1979-09-20 CA CA000336039A patent/CA1143681A/fr not_active Expired
- 1979-09-20 CH CH849779A patent/CH643575A5/fr not_active IP Right Cessation
- 1979-09-21 SE SE7907857A patent/SE7907857L/ not_active Application Discontinuation
- 1979-09-21 JP JP12188279A patent/JPS5544396A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IT7968841A0 (it) | 1979-09-20 |
| IT1119166B (it) | 1986-03-03 |
| JPS5544396A (en) | 1980-03-28 |
| PT70194A (fr) | 1979-10-01 |
| ATA615579A (de) | 1984-05-15 |
| EP0009430B1 (fr) | 1982-03-17 |
| FR2436791A1 (fr) | 1980-04-18 |
| CH643575A5 (fr) | 1984-06-15 |
| EP0009430A1 (fr) | 1980-04-02 |
| DE2962292D1 (en) | 1982-04-15 |
| CA1143681A (fr) | 1983-03-29 |
| BE878846A (fr) | 1980-03-18 |
| SE7907857L (sv) | 1980-03-23 |
| FR2436791B1 (pt) | 1981-02-13 |
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