LU503414B1 - Clean Dietary Fiber and Its Preparation Method - Google Patents

Clean Dietary Fiber and Its Preparation Method Download PDF

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Publication number
LU503414B1
LU503414B1 LU503414A LU503414A LU503414B1 LU 503414 B1 LU503414 B1 LU 503414B1 LU 503414 A LU503414 A LU 503414A LU 503414 A LU503414 A LU 503414A LU 503414 B1 LU503414 B1 LU 503414B1
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Luxembourg
Prior art keywords
powder
bagasse
mixed
clean
dietary fiber
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LU503414A
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French (fr)
Inventor
Jianjun Lu
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Univ Zhejiang
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Birds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a clean dietary fiber and its preparation method, which are relate to the technical field of pig feed. The clean dietary fiber is prepared from 30-50% of bamboo powder, 20-40% of grape branches and leaves powder, 10-30% of bagasse powder, 0.6-0.8% of urea, 0.05-0.2% of mixed microbial inoculum and 0.5-5% of enzymolysis liquid. The invention makes full use of bamboo fiber, grape branches and leaves and bagasse, and realizes efficient utilization of resources. At the same time, when the clean dietary fiber is added to the pig feed, the palatability and nutritional value of the feed can be significantly improved.

Description

DESCRIPTION LUS03414
Clean Dietary Fiber and Its Preparation Method
TECHNICAL FIELD
The invention belongs to the technical field of pig feed, in particular to a clean dietary fiber and its preparation method.
BACKGROUND
Dietary fiber is considered to be the seventh nutrient of animals, and it is particularly important to supplement proper dietary fiber to improve the intestinal health of animals.
At present, dietary fiber in pigs mainly includes bran, green feed, bean skin, dregs and so on, but these are perishable in production and application, and the mycotoxin exceeds the standard, which has adverse effects on the health of sows. In order to reduce the harm of mycotoxin to sows from the source, promote the intestinal peristalsis of sows, promote the growth of beneficial bacteria and inhibit the growth of harmful bacteria, it is of great practical significance to prepare new functional clean dietary fiber for pigs under the current intensive large-scale pig breeding conditions in China.
Bamboo stems are rich in fibrous tissues, and the chemical components are mainly composed of cellulose, hemicellulose and lignin. Vineyard pruning produces a large number of discarded branches and leaves every year, and most of these resources are piled up or burned at will, which has not been effectively developed and utilized, resulting in serious resource waste and environmental pollution. The rich organic matter and nutrient elements in grape branches and leaves are processed and converted into high-quality fiber feed, which has important utilization value; Bagasse is the residue of sugar cane after being squeezed in a sugar factory, which is abundant, centralized in source and low in price. Bagasse is a valuable feed resource, and 70% ~ 80% of bagasse is directly used as boiler fuel in the sugar factory, which is not effectively used, resulting in resource waste and certain pollution to the ecological environment.
Therefore, how to use these resources as raw materials for processing dietary fibék503414 has high economic value.
SUMMARY
Based on this, the present invention provides a clean dietary fiber and its preparation method, which is a clean dietary fiber made from bamboo powder, grape branches and leaves and bagasse through fermentation. The clean dietary fiber of the invention can improve the palatability and physical and chemical properties of the feed when added to the pig feed.
According to the invention, the clean dietary fiber comprises the following raw materials in percentage by mass: 30-50% of bamboo powder, 20-40% of grape branches and leaves powder, 10-30% of bagasse powder, 0.6-0.8% of urea, 0.05-0.2% of mixed microbial inoculum, 0.5-5% of enzymolysis liquid,
The sum of the mass percentages of the above raw materials is 100%;
The mixed microbial inoculum is formed by mixing plant lactic acid bacteria,
Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, Bacteroides amylophilus and cellulase;
The enzymolysis liquid is prepared by adding 300g of cellulase (1,500,000U/g) and 300g of hemicellulase (4500U/g) into 5kg distilled water.
Preferably, the active bacteria content in the plant lactic acid bacteria powder is 2*10°cfu/g, the active bacteria content in Saccharomyces cerevisiae powder is 2*10"°cfu/g, the active bacteria content in Bacillus subtilis powder is 1*10""cfu/g, the active bacteria content in Bifidobacterium powder is 1*10"°cfu/g and the active bacteria content in Bacteroides amylophilus powder is 5*10""cfu/g.
More preferably, the mass percentages of lactic acid bacteria powder,
Saccharomyces cerevisiae powder, Bacillus subtilis powder, Bifidobacterium powder and Bacteroides amylophilus powder in the mixed microbial inoculum are 20-40%U503414 10-20%, 15-30%, 15-30% and 15-30% respectively.
The preparation method of the clean dietary fiber comprises that following steps: (1) Pretreatment of bamboo stems: crush fresh bamboo stems, and control the water content to be 30-35% to obtain bamboo powder; (2) Pretreatment of grape branches and leaves: crush the freshly trimmed grape branches and leaves, and control the water content to be 30-35% to obtain grape branches and leaves powder; (3) Pretreatment of bagasse: crush fresh bagasse, add 2mol/L hydrochloric acid solution to adjust the pH value of bagasse to be 4-6, stand for 1 day, then adjust the pH value of bagasse to be neutral, and control the water content to be 30-35% to obtain bagasse powder; (4) Mix and crush the raw materials: mix bamboo powder, grape branches and leaves powder and bagasse powder, add urea in proportion, uniformly mix, crush, and sieve with a 10-30 meshes sieve to obtain the plant fiber mixture; (5) Sterilization: sterilize the plant fiber mixture at high temperature; (6) Mix the mixed microbial inoculum and the enzymolysis liquid in proportion to obtain the mixed fermentation microbial inoculum: (7) Mixing: cool the plant fiber mixed material sterilized in the step (5) to 30-40°C, add the mixed fermentation inoculum in the step (6), uniformly stir, and adjust the water content of the fermented material to 25-35% to obtain the mixed fermented material; (8) Carry out vacuum constant temperature fermentation on the mixed fermented material, wherein the fermentation temperature is 25-35°C, and the fermentation time is 120-300h, and obtain the clean dietary fiber after the fermentation.
Compared with the prior art, the invention has the following beneficial effects:
According to the invention, the rich fiber content of bamboo stems, the high protein content of grape branches and leaves and the sugar content in bagasse are fully utilized, and three kinds of mixed fibers of bamboo fiber, grape branches and leaves and bagasse are processed and modified by biological fermentation to prepare clean dietary fibers for pigs. Compared with traditional fiber feed raw materials, the clean dietary fibers have no mycotoxin, strong fermentability and water swelling, and stable componentsU503414
After fermentation, the anti-nutritional factor content of the mixed fibers is not only reduced, but also the contents of organic acids, beneficial bacteria and the like are increased, and flavor compounds are generated. When the clean dietary fiber of the invention is added to the pig feed (the addition amount is 1-6wt%), the palatability and physical and chemical properties of the feed can be significantly improved.
DETAILED DESCRIPTION OF THE INVENTION
The present invention will be further explained with specific embodiments.
Embodiment 1
The clean dietary fiber is composed of the following raw materials in percentage by mass: 43.9% of bamboo powder, 30% of grape branches and leaves powder, 21.5% of bagasse powder, 0.6% of urea, 0.05% of mixed microbial inoculum and 3.95% of enzymolysis liquid.
Wherein, the mixed microbial inoculum is formed by mixing plant lactic acid bacteria,
Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, Bacteroides amylophilus and cellulase; And the mass percentages of lactic acid bacteria powder, Saccharomyces cerevisiae powder, Bacillus subtilis powder, Bifidobacterium powder and Bacteroides amylophilus powder in the mixed microbial inoculum are 25%, 15%, 20%, 20% and 20% respectively.
The enzymolysis liquid is prepared by adding 300g of cellulase (1,500,000U/g) and 300g of hemicellulase (4500U/g) into 5kg distilled water.
The preparation method of the clean dietary fiber comprises that following steps: (1) Pretreatment of bamboo stems: crush fresh bamboo stems, and control the water content to be 31% to obtain bamboo powder; (2) Pretreatment of grape branches and leaves: crush the freshly trimmed grape branches and leaves, and control the water content to be 31% to obtain grape branches and leaves powder;
(3) Pretreatment of bagasse: crush fresh bagasse, add 2mol/L hydrochloric acld/503414 solution to adjust the pH value of bagasse to be 5, stand for 1 day, then adjust the pH value of bagasse to be neutral, and control the water content to be 30-35% to obtain bagasse powder; (4) Mix and crush the raw materials: mix bamboo powder, grape branches and leaves powder and bagasse powder, add urea in proportion, uniformly mix, crush, and sieve with a 20 meshes sieve to obtain the plant fiber mixture; (5) Sterilization: sterilize the plant fiber mixture by high-pressure steam sterilization, wherein the steam pressure is 1atm, the temperature is 120°C, and the time is 30min; (6) Mix the mixed microbial inoculum and the enzymolysis liquid in proportion to obtain the mixed fermentation microbial inoculum: (7) Mixing: cool the plant fiber mixed material sterilized in the step (5) to 35°C, add the mixed fermentation inoculum in the step (6), uniformly stir, and adjust the water content of the fermented material to 32% to obtain the mixed fermented material; (8) Carry out vacuum constant temperature fermentation on the mixed fermented material, wherein the fermentation temperature is 30°C, and the fermentation time is 200h, and obtain the clean dietary fiber after the fermentation. And the crude fiber content is more than or equal to 65%, and moisture content is less than or equal to 30%.
Embodiment 2
The clean dietary fiber is composed of the following raw materials in percentage by mass: 50% of bamboo powder, 25% of grape branches and leaves powder, 20% of bagasse powder, 0.7% of urea, 0.1% of mixed microbial inoculum and 4.2% of enzymolysis liquid.
Wherein, the mixed microbial inoculum is formed by mixing plant lactic acid bacteria,
Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, Bacteroides amylophilus and cellulase; And the mass percentages of lactic acid bacteria powder, Saccharomyces cerevisiae powder, Bacillus subtilis powder, Bifidobacterium powder and Bacteroides amylophilus powder in the mixed microbial inoculum are 30%, 20%, 15%, 15% and 20% respectively.
The enzymolysis liquid is prepared by adding 300g of cellulase (1,500,000U/g) and/503414 300g of hemicellulase (4500U/g) into 5kg distilled water.
The preparation method of the clean dietary fiber comprises that following steps: (1) Pretreatment of bamboo stems: crush fresh bamboo stems, and control the water content to be 31% to obtain bamboo powder; (2) Pretreatment of grape branches and leaves: crush the freshly trimmed grape branches and leaves, and control the water content to be 31% to obtain grape branches and leaves powder; (3) Pretreatment of bagasse: crush fresh bagasse, add 2mol/L hydrochloric acid solution to adjust the pH value of bagasse to be 5, stand for 1 day, then adjust the pH value of bagasse to be neutral, and control the water content to be 30-35% to obtain bagasse powder; (4) Mix and crush the raw materials: mix bamboo powder, grape branches and leaves powder and bagasse powder, add urea in proportion, uniformly mix, crush, and sieve with a 10 meshes sieve to obtain the plant fiber mixture; (5) Sterilization: sterilize the plant fiber mixture by high-pressure steam sterilization, wherein the steam pressure is 1atm, the temperature is 120°C, and the time is 30min; (6) Mix the mixed microbial inoculum and the enzymolysis liquid in proportion to obtain the mixed fermentation microbial inoculum: (7) Mixing: cool the plant fiber mixed material sterilized in the step (5) to 35°C, add the mixed fermentation inoculum in the step (6), uniformly stir, and adjust the water content of the fermented material to 32% to obtain the mixed fermented material; (8) Carry out vacuum constant temperature fermentation on the mixed fermented material, wherein the fermentation temperature is 33°C, and the fermentation time is 150h, and obtain the clean dietary fiber after the fermentation. And the crude fiber content is more than or equal to 65%, and moisture content is less than or equal to 30%.
Embodiment 3
The clean dietary fiber is composed of the following raw materials in percentage by mass:
45% of bamboo powder, 35% of grape branches and leaves powder, 18.5% b#503414 bagasse powder, 0.8% of urea, 0.2% of mixed microbial inoculum and 0.5% of enzymolysis liquid.
Wherein, the mixed microbial inoculum is formed by mixing plant lactic acid bacteria,
Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, Bacteroides amylophilus and cellulase; And the mass percentages of lactic acid bacteria powder, Saccharomyces cerevisiae powder, Bacillus subtilis powder, Bifidobacterium powder and Bacteroides amylophilus powder in the mixed microbial inoculum are 28%, 12%, 20%, 20% and 20% respectively.
The enzymolysis liquid is prepared by adding 300g of cellulase (1,500,000U/g) and 300g of hemicellulase (4500U/g) into 5kg distilled water.
The preparation method of the clean dietary fiber comprises that following steps: (1) Pretreatment of bamboo stems: crush fresh bamboo stems, and control the water content to be 31% to obtain bamboo powder; (2) Pretreatment of grape branches and leaves: crush the freshly trimmed grape branches and leaves, and control the water content to be 31% to obtain grape branches and leaves powder; (3) Pretreatment of bagasse: crush fresh bagasse, add 2mol/L hydrochloric acid solution to adjust the pH value of bagasse to be 5, stand for 1 day, then adjust the pH value of bagasse to be neutral, and control the water content to be 30-35% to obtain bagasse powder; (4) Mix and crush the raw materials: mix bamboo powder, grape branches and leaves powder and bagasse powder, add urea in proportion, uniformly mix, crush, and sieve with a 20 meshes sieve to obtain the plant fiber mixture; (5) Sterilization: sterilize the plant fiber mixture by high-pressure steam sterilization, wherein the steam pressure is 1atm, the temperature is 120°C, and the time is 30min; (6) Mix the mixed microbial inoculum and the enzymolysis liquid in proportion to obtain the mixed fermentation microbial inoculum:
(7) Mixing: cool the plant fiber mixed material sterilized in the step (5) to 35°C, add/503414 the mixed fermentation inoculum in the step (6), uniformly stir, and adjust the water content of the fermented material to 32% to obtain the mixed fermented material; (8) Carry out vacuum constant temperature fermentation on the mixed fermented material, wherein the fermentation temperature is 31°C, and the fermentation time is 180h, and obtain the clean dietary fiber after the fermentation. And the crude fiber content is more than or equal to 65%, and moisture content is less than or equal to 30%.
It should be noted that the above-mentioned embodiments are only a few specific embodiments of the present invention, and it is obvious that the present invention is not limited to the above-mentioned embodiments, and there may be other variations. All variations directly or indirectly derived by those skilled in the art from the disclosure of the present invention should be considered as the protection scope of the present invention.

Claims (4)

CLAIMS LU503414
1. A clean dietary fiber is characterized in that it comprises the following raw materials in percentage by mass: 30-50% of bamboo powder, 20-40% of grape branches and leaves powder, 10-30% of bagasse powder,
0.6-0.8% of urea,
0.05-0.2% of mixed microbial inoculum,
0.5-5% of enzymolysis liquid, the sum of the mass percentages of the above raw materials is 100%; the mixed microbial inoculum is formed by mixing plant lactic acid bacteria, Saccharomyces cerevisiae, Bacillus subtilis, Bifidobacterium, Bacteroides amylophilus and cellulase; the enzymolysis liquid is prepared by adding 300g of cellulase and 300g of hemicellulase into 5kg distilled water.
2. The clean dietary fiber according to claim 1 is characterized in that the active bacteria content in the plant lactic acid bacteria powder is 2*10°cfu/g, the active bacteria content in Saccharomyces cerevisiae powder is 2*10"°cfu/g, the active bacteria content in Bacillus subtilis powder is 1*10"cfu/g, the active bacteria content in Bifidobacterium powder is 1*10"°cfu/g and the active bacteria content in Bacteroides amylophilus powder is 5*10""cfu/g.
3. The clean dietary fiber according to claim 2 is characterized in that the mass percentages of lactic acid bacteria powder, Saccharomyces cerevisiae powder, Bacillus subtilis powder, Bifidobacterium powder and Bacteroides amylophilus powder in the mixed microbial inoculum are 20-40%, 10-20%, 15-30%, 15-30% and 15-30% respectively.
4. The preparation method of the clean dietary fiber according to any of claims 1-4 is characterized in that it comprises that following steps: (1) pretreatment of bamboo stems: crush fresh bamboo stems, and control the water content to be 30-35% to obtain bamboo powder;
(2) pretreatment of grape branches and leaves: crush the freshly trimmed grapé/503414 branches and leaves, and control the water content to be 30-35% to obtain grape branches and leaves powder;
(3) pretreatment of bagasse: crush fresh bagasse, add 2mol/L hydrochloric acid solution to adjust the pH value of bagasse to be 4-6, stand for 1 day, then adjust the pH value of bagasse to be neutral, and control the water content to be 30-35% to obtain bagasse powder;
(4) mix and crush the raw materials: mix bamboo powder, grape branches and leaves powder and bagasse powder, add urea in proportion, uniformly mix, crush, and sieve with a 10-30 meshes sieve to obtain the plant fiber mixture;
(5) sterilization: sterilize the plant fiber mixture at high temperature;
(6) mix the mixed microbial inoculum and the enzymolysis liquid in proportion to obtain the mixed fermentation microbial inoculum:
(7) mixing: cool the plant fiber mixed material sterilized in the step (5) to 30-40°C, add the mixed fermentation inoculum in the step (6), uniformly stir, and adjust the water content of the fermented material to 25-35% to obtain the mixed fermented material;
(8) carry out vacuum constant temperature fermentation on the mixed fermented material, wherein the fermentation temperature is 25-35°C, and the fermentation time is 120-300h, and obtain the clean dietary fiber after the fermentation.
LU503414A 2023-02-01 2023-02-01 Clean Dietary Fiber and Its Preparation Method LU503414B1 (en)

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LU503414A LU503414B1 (en) 2023-02-01 2023-02-01 Clean Dietary Fiber and Its Preparation Method

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LU503414B1 true LU503414B1 (en) 2023-08-01

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