LT3422B - Method of preparing lysozyme dimers - Google Patents
Method of preparing lysozyme dimers Download PDFInfo
- Publication number
- LT3422B LT3422B LTIP594A LTIP594A LT3422B LT 3422 B LT3422 B LT 3422B LT IP594 A LTIP594 A LT IP594A LT IP594 A LTIP594 A LT IP594A LT 3422 B LT3422 B LT 3422B
- Authority
- LT
- Lithuania
- Prior art keywords
- lysozyme
- solution
- fractions
- monomer
- dimer
- Prior art date
Links
- 102000016943 Muramidase Human genes 0.000 title claims abstract description 84
- 108010014251 Muramidase Proteins 0.000 title claims abstract description 84
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 title claims abstract description 84
- 229960000274 lysozyme Drugs 0.000 title claims abstract description 84
- 239000004325 lysozyme Substances 0.000 title claims abstract description 84
- 235000010335 lysozyme Nutrition 0.000 title claims abstract description 84
- 239000000539 dimer Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 37
- 239000000243 solution Substances 0.000 claims abstract description 63
- 238000010828 elution Methods 0.000 claims abstract description 31
- 239000000178 monomer Substances 0.000 claims abstract description 24
- 238000006471 dimerization reaction Methods 0.000 claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 230000008878 coupling Effects 0.000 claims abstract description 10
- 238000010168 coupling process Methods 0.000 claims abstract description 10
- 238000005859 coupling reaction Methods 0.000 claims abstract description 10
- 239000007853 buffer solution Substances 0.000 claims abstract description 3
- 239000000872 buffer Substances 0.000 claims description 19
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 10
- 239000005695 Ammonium acetate Substances 0.000 claims description 10
- 235000019257 ammonium acetate Nutrition 0.000 claims description 10
- 229940043376 ammonium acetate Drugs 0.000 claims description 10
- 238000005342 ion exchange Methods 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000001502 gel electrophoresis Methods 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 7
- KDQPSPMLNJTZAL-UHFFFAOYSA-L disodium hydrogenphosphate dihydrate Chemical compound O.O.[Na+].[Na+].OP([O-])([O-])=O KDQPSPMLNJTZAL-UHFFFAOYSA-L 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 229920002684 Sepharose Polymers 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 229920005654 Sephadex Polymers 0.000 claims description 4
- 239000012507 Sephadex™ Substances 0.000 claims description 4
- FRTGEIHSCHXMTI-UHFFFAOYSA-N dimethyl octanediimidate Chemical group COC(=N)CCCCCCC(=N)OC FRTGEIHSCHXMTI-UHFFFAOYSA-N 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 3
- 241000272525 Anas platyrhynchos Species 0.000 claims 2
- 239000004615 ingredient Substances 0.000 claims 1
- 230000001472 cytotoxic effect Effects 0.000 abstract description 7
- 230000003612 virological effect Effects 0.000 abstract description 6
- 208000035143 Bacterial infection Diseases 0.000 abstract description 5
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract description 2
- 231100000433 cytotoxic Toxicity 0.000 abstract description 2
- 239000003456 ion exchange resin Substances 0.000 abstract description 2
- 229920003303 ion-exchange polymer Polymers 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 238000004440 column chromatography Methods 0.000 abstract 1
- 230000000447 dimerizing effect Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 238000001962 electrophoresis Methods 0.000 description 11
- 239000008363 phosphate buffer Substances 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 239000000203 mixture Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 241000191938 Micrococcus luteus Species 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 238000000638 solvent extraction Methods 0.000 description 5
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000000840 anti-viral effect Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 2
- GUGOEEXESWIERI-UHFFFAOYSA-N Terfenadine Chemical compound C1=CC(C(C)(C)C)=CC=C1C(O)CCCN1CCC(C(O)(C=2C=CC=CC=2)C=2C=CC=CC=2)CC1 GUGOEEXESWIERI-UHFFFAOYSA-N 0.000 description 2
- 239000002390 adhesive tape Substances 0.000 description 2
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000001387 anti-histamine Effects 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 239000000739 antihistaminic agent Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 239000007862 dimeric product Substances 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 238000009499 grossing Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- -1 dimethylsulfurimidate Chemical compound 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Communicable Diseases (AREA)
- Genetics & Genomics (AREA)
- Oncology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Rheumatology (AREA)
- Pain & Pain Management (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002073351A CA2073351C (en) | 1990-01-08 | 1990-01-08 | Lysozyme dimers and method of preparing them |
| RO92-0931A RO114805B1 (ro) | 1990-01-08 | 1990-01-08 | Dimer de lizozim purificat si procedeu de obtinere a acestuia |
| HU9202250A HU214509B (hu) | 1990-01-08 | 1990-01-08 | Eljárás tisztított lizozim dimerek előállítására |
| SG1996007945A SG49871A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| PCT/US1990/000140 WO1991010731A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
| OA60242A OA09706A (en) | 1990-01-08 | 1992-07-07 | Method of preparing lysozyme dimers. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LTIP594A LTIP594A (en) | 1994-12-27 |
| LT3422B true LT3422B (en) | 1995-09-25 |
Family
ID=33136306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTIP594A LT3422B (en) | 1990-01-08 | 1993-06-02 | Method of preparing lysozyme dimers |
Country Status (20)
| Country | Link |
|---|---|
| EP (1) | EP0509984B1 (ro) |
| JP (1) | JP2732514B2 (ro) |
| KR (1) | KR0158216B1 (ro) |
| AU (1) | AU654651B2 (ro) |
| BG (1) | BG61040B1 (ro) |
| BR (1) | BR9007971A (ro) |
| CA (1) | CA2073351C (ro) |
| DE (1) | DE69032375T2 (ro) |
| DK (1) | DK0509984T3 (ro) |
| ES (1) | ES2118719T3 (ro) |
| FI (1) | FI101399B1 (ro) |
| HU (1) | HU214509B (ro) |
| LT (1) | LT3422B (ro) |
| LV (1) | LV10119B (ro) |
| MC (2) | MC2243A1 (ro) |
| NO (1) | NO302484B1 (ro) |
| OA (1) | OA09706A (ro) |
| RO (1) | RO114805B1 (ro) |
| SG (1) | SG49871A1 (ro) |
| WO (1) | WO1991010731A1 (ro) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL173978B1 (pl) * | 1992-07-13 | 1998-05-29 | Nika Health Products Ltd | Sposób oddziaływania na wydzielanie cytokin w hodowli limfocytów krwi obwodowej |
| US6183742B1 (en) | 1992-07-13 | 2001-02-06 | Nika Health Products, Limited | Applications of lysozyme dimer |
| DZ1964A1 (fr) * | 1995-01-13 | 2002-10-15 | Nika Health Products Ltd | Nouvelle applications d'un dimère de lysozyme. |
| US6123937A (en) * | 1997-03-14 | 2000-09-26 | Nika Health Products, Limited | Applications of lysozyme dimer |
| US7348301B2 (en) * | 2006-02-16 | 2008-03-25 | Buckman Laboratories International, Inc. | Lysozyme-based method and composition to control the growth of microorganisms in aqueous systems |
| WO2013052643A1 (en) * | 2011-10-05 | 2013-04-11 | The Rockefeller University | Dimeric bacteriophage lysins |
| CN111751468A (zh) * | 2020-07-03 | 2020-10-09 | 上海艾魁英生物科技有限公司 | 溶菌酶二聚体的纯化及其标准品制备的方法 |
| CN118995673B (zh) * | 2024-08-08 | 2025-08-12 | 南宁庞博生物工程有限公司 | 热催化与化学修饰联合应用制备溶菌酶二聚体的方法与应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8801785B2 (en) | 2006-10-04 | 2014-08-12 | Seaspine, Inc. | Articulating spinal implant |
-
1990
- 1990-01-08 CA CA002073351A patent/CA2073351C/en not_active Expired - Lifetime
- 1990-01-08 HU HU9202250A patent/HU214509B/hu unknown
- 1990-01-08 MC MC90US9000140D patent/MC2243A1/xx unknown
- 1990-01-08 ES ES90902033T patent/ES2118719T3/es not_active Expired - Lifetime
- 1990-01-08 DE DE69032375T patent/DE69032375T2/de not_active Expired - Fee Related
- 1990-01-08 WO PCT/US1990/000140 patent/WO1991010731A1/en not_active Ceased
- 1990-01-08 AU AU48474/90A patent/AU654651B2/en not_active Ceased
- 1990-01-08 JP JP2502143A patent/JP2732514B2/ja not_active Expired - Fee Related
- 1990-01-08 MC MC90US9000140D patent/MC2242A1/xx unknown
- 1990-01-08 KR KR1019920701566A patent/KR0158216B1/ko not_active Expired - Fee Related
- 1990-01-08 DK DK90902033T patent/DK0509984T3/da active
- 1990-01-08 RO RO92-0931A patent/RO114805B1/ro unknown
- 1990-01-08 SG SG1996007945A patent/SG49871A1/en unknown
- 1990-01-08 EP EP90902033A patent/EP0509984B1/en not_active Expired - Lifetime
- 1990-01-08 BR BR909007971A patent/BR9007971A/pt not_active Application Discontinuation
-
1992
- 1992-07-03 NO NO922626A patent/NO302484B1/no not_active IP Right Cessation
- 1992-07-07 FI FI923123A patent/FI101399B1/fi active
- 1992-07-07 OA OA60242A patent/OA09706A/en unknown
- 1992-07-07 BG BG96578A patent/BG61040B1/bg unknown
- 1992-12-28 LV LVP-92-506A patent/LV10119B/en unknown
-
1993
- 1993-06-02 LT LTIP594A patent/LT3422B/lt not_active IP Right Cessation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8801785B2 (en) | 2006-10-04 | 2014-08-12 | Seaspine, Inc. | Articulating spinal implant |
Non-Patent Citations (1)
| Title |
|---|
| J O THOMAS ET AL.: "An octamer of histones in chromatin and free in solution", PROC. NATL. ACAD. SCI. USA, 1975, pages 2626 |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SU1012786A3 (ru) | Способ получени протеинового комплекса,стимулирующего секрецию инсулина | |
| Barteling | A simple method for the preparation of agarose | |
| AU654651B2 (en) | Method of preparing lysozyme dimers | |
| Phillips et al. | Techniques for the fractionation of microbiologically active peptides derived from casein | |
| Vitković et al. | Purification of the extracellular protease of Bacillus licheniformis and its inhibition by bacitracin | |
| Willstätter | Problems and methods in enzyme research | |
| US5314816A (en) | Method of preparing lysozyme dimers | |
| RU2067617C1 (ru) | Способ получения димера лизоцима | |
| US6316236B1 (en) | Lysozyme dimer | |
| SAKURAI et al. | Isolation of a peptidyl factor controlling sexual agglutination in Saccharomyces cerevisiae | |
| CA2073350C (en) | Ribonuclease dimers and method of preparing them | |
| CA2067973A1 (en) | Yeast-derived mitogen | |
| KAWASAKI et al. | Isolation and some characteristics of placental alkaline phosphatase | |
| SU767121A1 (ru) | Способ выделени и очистки трипсина и химотрипсина | |
| Shooter et al. | Macromolecular aspects of the nerve growth factor proteins | |
| JPH0995407A (ja) | 成長促進物質 | |
| JPS6169730A (ja) | 可溶性蛋白質ｆｂｐ及びその製造方法 | |
| GB1188656A (en) | Substance Having Growth Promoting and Metabolism Promoting Action and Process for Recovering It | |
| JPH0143548B2 (ro) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM9A | Lapsed patents |
Effective date: 20020602 |