KR970000018A - Manufacturing method of ginger artificial seedlings - Google Patents

Manufacturing method of ginger artificial seedlings Download PDF

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Publication number
KR970000018A
KR970000018A KR1019950016656A KR19950016656A KR970000018A KR 970000018 A KR970000018 A KR 970000018A KR 1019950016656 A KR1019950016656 A KR 1019950016656A KR 19950016656 A KR19950016656 A KR 19950016656A KR 970000018 A KR970000018 A KR 970000018A
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South Korea
Prior art keywords
ginger
artificial
seedlings
medium
growth
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KR1019950016656A
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Korean (ko)
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KR0160086B1 (en
Inventor
장영선
최인록
김태수
노경희
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김광희
농촌진흥청
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Priority to KR1019950016656A priority Critical patent/KR0160086B1/en
Publication of KR970000018A publication Critical patent/KR970000018A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002Culture media for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0025Culture media for plant cell or plant tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04Plant cells or tissues
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S47/00Plant husbandry
    • Y10S47/03Propagation of plant by cuttings

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

본 발명은 생강농사를 주로 하는 재배농가에서 별도의 선별 및 저장 과정을 거치지 않고 파종용 종강을 연중 확보할 수있는 방법에 관한 것이다. 본 발명에서는 생강의 인공종묘의 눈이 1 내지 2cm 정도 신장하였을 때 생장점을 절취, 치상하여 생육시킨 다음, 분화된 식물체의 근경비대를 유도하고 순화시켜 파종용 종강을 제조하였다. 본 발명의 종강용 생강 인공종묘 제조방법에 의하면, 경제적이고도 간편한 방법으로 양질의 종강을 연중 생산할 수 있다.The present invention relates to a method capable of securing year-round seedlings without going through a separate screening and storage process in a cultivation farm mainly do ginger farming. In the present invention, when the eyes of the artificial seedlings of ginger stretched by about 1 to 2 cm, the growth point was cut, healed, and grown, and the seedling for seeding was prepared by inducing and purifying the root diameter of differentiated plants. According to the ginger artificial seedling manufacturing method of the present invention according to the present invention, it is possible to produce high-quality seed steel throughout the year by an economical and convenient method.

Description

종강용 생강 인공종묘의 제조방법Manufacturing method of ginger artificial seedlings

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (9)

(i) 생강의 눈이 1 내지 2cm 신장하였을 때 생장점을 절취하여 영양배지에 치상하여 기관의 분화를 유도하는 단계;(i) cutting the growth point when the eyes of ginger are 1-2 cm elongated and injured in the nutrient medium to induce organ differentiation; (ⅱ) 전기 기관의 분화가 유도된 분화식물체를 인공배지에서 생육시키고 근경비대를 유도하기 위해 영양배지에 이식시키는 단계; 및,(Ii) growing the differentiated plants induced by the differentiation of the electric organs in artificial media and transplanting them into nutrient media to induce myomegaly; And, (ⅲ) 전기 근경비대가 형성된 유식물체를 순화시켜 포장에서 재배할 수 있는 식물체로 성장시키는 단계를 포함하는 종강용 생강 인공종묘의 제조방법.(Iii) A method of producing artificial ginger ginger seedlings comprising the step of purifying the seedlings with electric myomegalia formed into plants that can be grown in the field. 제1항에 있어서, 생장점의 치상을 위한 영양배지는 수크로스 30g/1, NAA(α-naphthaleneacetic acid)0.2mg/1, BAP(6-benzylamino purine) 1.0mg/1 및 아가(agar) 8g/1를 함유하는 MS 기본배지(pH 5.6 내지 5.8)인 것을 특징으로 하는 방법.The nutrient medium for dentate growth of claim 1 is sucrose 30 g / 1, 0.2 mg / αA-naphthaleneacetic acid (NAA), 1.0 mg / 6 BAP (6-benzylamino purine) and 8 g / agar (Agar). MS base medium (pH 5.6 to 5.8) containing 1, characterized in that. 제1항에 있어서, 절치된 생장점을 영양배지내에 25℃의 온도조건, 16시간 명배양 조건으로 60일간 배양하는 것을 특징으로 하는 방법.The method of claim 1, wherein the growth point is incubated in a nutrient medium for 60 days at 25 ℃, 16 hours light culture conditions. 제1항에 있어서, 분화식물체의 생육을 위한 인공배지는 수크로스 30g/1, NAA(α-naphthaleneacetic acid)0.5mg/1, BAP(6-benzylamino purine) 2.0mg/1 및 아가(agar) 8g/1를 함유하는 MS 기본배지(pH 5.6 내지 5.8)인 것을 특징으로 하는 방법.The method according to claim 1, wherein the artificial medium for the growth of differentiated plants is sucrose 30g / 1, 0.5A / α-naphthaleneacetic acid (NAA), 2.0mg / 1 and 6g agar (6-benzylamino purine) MS base medium (pH 5.6 to 5.8) containing / 1. 제1항에 있어서, 분화식물체를 25±1℃의 온도조건, 16시간 명배양 조건으로 60일간 생육시키는 것을 특징으로 하는 방법.The method according to claim 1, wherein the differentiated plants are grown for 60 days under conditions of 25 ± 1 ° C. and 16 hours of light culture. 제1항에 있어서, 분화식물체의 근경비대 유도를 위한 영양 배지는 수크로스 90g/1, 활성탄 2g/1, C.C.C.(chlorocholine chloride) 500mg/1, NAA(α-naphthaleneacetic acid)1 및 BAP(6-benzylamino purine) 2.0mg/1를 함유하는 MS 기본배지(pH 5.6 내지 5.8)인 것을 특징으로 하는 방법.The method of claim 1, wherein the nutrient medium for the induction of myocardial hypertrophy of the differentiated plant is sucrose 90g / 1, activated carbon 2g / 1, CCC (chlorocholine chloride) 500mg / 1, NAA (α-naphthaleneacetic acid) 1 and BAP (6- benzylamino purine) MS base medium (pH 5.6 to 5.8) containing 2.0mg / 1. 제1항에 있어서, 근경비대가 형성된 유식물체를 주간 25℃와 야간 20℃의 온도조건으로 피트(peat)의 상토를 사용하여 순화처리하는 것을 특징으로 하는 방법.2. The method according to claim 1, wherein the seedlings in which the rhizome is formed are purified using a top soil of peat at a temperature of 25 ° C. and 20 ° C. at night. 제1항의 방법에 의해 제조된 종강용 생강 인공종묘를 파종하고 차광처리하에 재배하는 단계를 포함하는 생강의 재배방법.Cultivation method of ginger comprising the step of planting the seedling ginger artificial seedling prepared by the method of claim 1 and cultivating under shading treatment. 제8항에 있어서, 차광처리는 약 50%로 행하는 것을 특징으로 하는 방법.9. The method of claim 8, wherein the shading treatment is performed at about 50%. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950016656A 1995-06-21 1995-06-21 The method of cultivating ginger seedlings KR0160086B1 (en)

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Application Number Priority Date Filing Date Title
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KR0160086B1 KR0160086B1 (en) 1998-11-16

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100497869B1 (en) * 2002-12-12 2005-06-29 이창호 The automatic controlled yarn guide of knitting machine
CN102939903A (en) * 2012-11-23 2013-02-27 绍兴文理学院 Ginger detoxification, sterilization and rapid propagation method
CN111183862A (en) * 2019-12-30 2020-05-22 中国科学院水利部成都山地灾害与环境研究所 Method for eliminating continuous cropping obstacle of salvia miltiorrhiza
CN117158322A (en) * 2023-11-02 2023-12-05 潍坊理工学院 Tissue culture breeding rapid propagation seedling method based on ginger root

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100372949B1 (en) * 2000-02-10 2003-03-08 대한민국 (경상대학교 총장) Method for cultivation of garden products
KR100478734B1 (en) * 2002-10-08 2005-03-24 강경원 New breed of Cymbidium, Dae Guk
CN103348917B (en) * 2013-07-18 2014-11-12 湖北蔬谷农业科技有限公司 Rapid propagation method of ginger virus-free seedlings by one step
CN103651124B (en) * 2013-11-22 2016-07-20 青岛文创科技有限公司 A kind of abductive approach of plant regeneration of zingiber officinale
KR101653769B1 (en) 2014-09-25 2016-09-02 이성민 Method cultivating a ginger

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100497869B1 (en) * 2002-12-12 2005-06-29 이창호 The automatic controlled yarn guide of knitting machine
CN102939903A (en) * 2012-11-23 2013-02-27 绍兴文理学院 Ginger detoxification, sterilization and rapid propagation method
CN111183862A (en) * 2019-12-30 2020-05-22 中国科学院水利部成都山地灾害与环境研究所 Method for eliminating continuous cropping obstacle of salvia miltiorrhiza
CN111183862B (en) * 2019-12-30 2022-03-08 中国科学院水利部成都山地灾害与环境研究所 Method for eliminating continuous cropping obstacle of salvia miltiorrhiza
CN117158322A (en) * 2023-11-02 2023-12-05 潍坊理工学院 Tissue culture breeding rapid propagation seedling method based on ginger root
CN117158322B (en) * 2023-11-02 2024-01-26 潍坊理工学院 Tissue culture breeding rapid propagation seedling method based on ginger root

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