KR960023081A - 효소 표지 항체를 안정화시키는 희석액 - Google Patents

효소 표지 항체를 안정화시키는 희석액 Download PDF

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Publication number
KR960023081A
KR960023081A KR1019940037240A KR19940037240A KR960023081A KR 960023081 A KR960023081 A KR 960023081A KR 1019940037240 A KR1019940037240 A KR 1019940037240A KR 19940037240 A KR19940037240 A KR 19940037240A KR 960023081 A KR960023081 A KR 960023081A
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South Korea
Prior art keywords
enzyme
dilutions
labeled antibodies
labeled antibody
bovine serum
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KR1019940037240A
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English (en)
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KR100342935B1 (ko
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윤수희
김인수
김주호
최동섭
Original Assignee
성재갑
주식회사 Lg 화학
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Priority to KR1019940037240A priority Critical patent/KR100342935B1/ko
Publication of KR960023081A publication Critical patent/KR960023081A/ko
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Publication of KR100342935B1 publication Critical patent/KR100342935B1/ko

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 Ca2+와 낮은 농도(15% 미만)의 우형 혈청 알부민(fetal bovine serum)을 필수적으로 포함하는, 효소 면역분석법(enzyme immunoassay)에 사용되는 효소 표지 항체의 안정성을 높일 수 있는 희석액에 관한 것이다.

Description

효소 표지 항체를 안정화시키는 희석액
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 내지 제3도는 실시예 1 내지 3에 기재된 각 용액의 안정성을 효소 면역분석법(enzyme immunoassay, EIA)에서의 흡광도의 변화로써 측정한 결과를 나타낸 것이다.

Claims (3)

  1. Ca2+와 15% 이하의 우형 혈청 알부민(fetal bovine serum)을 필수적으로 포함하는 효소 표지 항체 희석액.
  2. 제1항에 있어서, Ca2+의 함량이 0.001% 이상인 효소 표지 항체 희석액.
  3. 제2항에 있어서, 0.01 내지 0.1% CaCl2, 1 내지 5% 우형 혈청 알부민, 5% 염소 혈청, 0.02% 티메로살(thimerosal), 0.05% 트윈-20, 1mM EDTA 및 0.5M NaCl을 함유하는 10mM 트리스 완충용액(pH 7.5)인 효소 표시 항체 희석액.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940037240A 1994-12-27 1994-12-27 효소표지항체를안정화시키는희석액 KR100342935B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940037240A KR100342935B1 (ko) 1994-12-27 1994-12-27 효소표지항체를안정화시키는희석액

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940037240A KR100342935B1 (ko) 1994-12-27 1994-12-27 효소표지항체를안정화시키는희석액

Publications (2)

Publication Number Publication Date
KR960023081A true KR960023081A (ko) 1996-07-18
KR100342935B1 KR100342935B1 (ko) 2002-11-18

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ID=37488337

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940037240A KR100342935B1 (ko) 1994-12-27 1994-12-27 효소표지항체를안정화시키는희석액

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KR (1) KR100342935B1 (ko)

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KR100342935B1 (ko) 2002-11-18

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