KR960009052B1 - Simultaneous preparation process of useful proteins from human urine - Google Patents
Simultaneous preparation process of useful proteins from human urine Download PDFInfo
- Publication number
- KR960009052B1 KR960009052B1 KR96008146A KR19960008146A KR960009052B1 KR 960009052 B1 KR960009052 B1 KR 960009052B1 KR 96008146 A KR96008146 A KR 96008146A KR 19960008146 A KR19960008146 A KR 19960008146A KR 960009052 B1 KR960009052 B1 KR 960009052B1
- Authority
- KR
- South Korea
- Prior art keywords
- chromatography
- human urine
- urokinase
- egf
- albumin
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 210000002700 urine Anatomy 0.000 title abstract 3
- 238000002360 preparation method Methods 0.000 title 1
- 102000009027 Albumins Human genes 0.000 abstract 3
- 108010088751 Albumins Proteins 0.000 abstract 3
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 abstract 3
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 abstract 3
- 238000001641 gel filtration chromatography Methods 0.000 abstract 3
- 229960005356 urokinase Drugs 0.000 abstract 3
- 102000009024 Epidermal Growth Factor Human genes 0.000 abstract 2
- 238000001042 affinity chromatography Methods 0.000 abstract 2
- 101710098940 Pro-epidermal growth factor Proteins 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 abstract 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 230000008018 melting Effects 0.000 abstract 1
- 238000002844 melting Methods 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 abstract 1
- 239000011592 zinc chloride Substances 0.000 abstract 1
- 235000005074 zinc chloride Nutrition 0.000 abstract 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR96008146A KR960009052B1 (en) | 1992-05-26 | 1996-03-25 | Simultaneous preparation process of useful proteins from human urine |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019920008884A KR960009051B1 (ko) | 1992-05-26 | 1992-05-26 | 사람의 뇨로부터의 유용 단백질의 동시 제조방법 |
KR96008146A KR960009052B1 (en) | 1992-05-26 | 1996-03-25 | Simultaneous preparation process of useful proteins from human urine |
Publications (1)
Publication Number | Publication Date |
---|---|
KR960009052B1 true KR960009052B1 (en) | 1996-07-10 |
Family
ID=19333576
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920008884A KR960009051B1 (ko) | 1992-05-26 | 1992-05-26 | 사람의 뇨로부터의 유용 단백질의 동시 제조방법 |
KR96008146A KR960009052B1 (en) | 1992-05-26 | 1996-03-25 | Simultaneous preparation process of useful proteins from human urine |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920008884A KR960009051B1 (ko) | 1992-05-26 | 1992-05-26 | 사람의 뇨로부터의 유용 단백질의 동시 제조방법 |
Country Status (2)
Country | Link |
---|---|
KR (2) | KR960009051B1 (ko) |
CN (1) | CN1037103C (ko) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1064049C (zh) * | 1995-10-05 | 2001-04-04 | 南京大学 | 血小板生成素制备工艺 |
CN1113896C (zh) * | 1998-01-04 | 2003-07-09 | 刘荣秀 | 用虾及其废弃物制备表皮生长因子的方法 |
CN1090971C (zh) * | 1999-10-26 | 2002-09-18 | 合肥永生制药有限公司 | 尿多酸肽组合物 |
CN1090970C (zh) * | 1999-10-26 | 2002-09-18 | 合肥永生制药有限公司 | 尿多酸肽组合物的制备方法 |
CN1088597C (zh) * | 1999-10-26 | 2002-08-07 | 合肥永生制药有限公司 | 尿多酸肽组合物及其用途 |
CN103509104A (zh) * | 2013-08-23 | 2014-01-15 | 扬州艾迪生物科技有限公司 | 一种规模化收集人尿蛋白的方法 |
CN103740687B (zh) * | 2013-11-30 | 2016-05-11 | 青岛康原药业有限公司 | 一种制备尿激酶粗品的方法 |
CN103694334B (zh) * | 2013-12-23 | 2015-10-21 | 扬州艾迪生物科技有限公司 | 一种制备hEGF粗制品的方法 |
CN104531648A (zh) * | 2014-12-23 | 2015-04-22 | 青岛康原药业有限公司 | 一种制备尿激酶粗品的方法 |
CN108333263A (zh) * | 2017-01-20 | 2018-07-27 | 北京蛋白质组研究中心 | 一种尿蛋白制备方法及尿蛋白质组的检测方法 |
CN112250753B (zh) * | 2020-10-28 | 2023-06-13 | 宁波博睿瀚达生物科技有限公司 | 一种游离吸附浓缩重组表皮生长因子的方法 |
-
1992
- 1992-05-26 KR KR1019920008884A patent/KR960009051B1/ko not_active IP Right Cessation
- 1992-08-28 CN CN92108938A patent/CN1037103C/zh not_active Expired - Fee Related
-
1996
- 1996-03-25 KR KR96008146A patent/KR960009052B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
CN1037103C (zh) | 1998-01-21 |
KR960009051B1 (ko) | 1996-07-10 |
CN1079226A (zh) | 1993-12-08 |
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G160 | Decision to publish patent application | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20020708 Year of fee payment: 9 |
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