KR950704467A - 알칼리 리파아제(alkaline lipase) - Google Patents

알칼리 리파아제(alkaline lipase)

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Publication number
KR950704467A
KR950704467A KR1019950702583A KR19950702583A KR950704467A KR 950704467 A KR950704467 A KR 950704467A KR 1019950702583 A KR1019950702583 A KR 1019950702583A KR 19950702583 A KR19950702583 A KR 19950702583A KR 950704467 A KR950704467 A KR 950704467A
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South Korea
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atcc
strain
lipase
dsm
griseus
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KR1019950702583A
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English (en)
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미요코 하시다
나오코 이케가미
마사노부 아보
유키코 타카무라
Original Assignee
안네 제케르
노보 노르디스크 아크티에 셀스카브
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Priority claimed from DK152992A external-priority patent/DK152992D0/da
Priority claimed from DK9693A external-priority patent/DK9693D0/da
Priority claimed from DK44293A external-priority patent/DK44293D0/da
Application filed by 안네 제케르, 노보 노르디스크 아크티에 셀스카브 filed Critical 안네 제케르
Publication of KR950704467A publication Critical patent/KR950704467A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/545—Streptomyces griseus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

고 알칼리성, 위치 비특이적인 리파아제를 Streptomyces 1군의 균주로부터 얻을 수 있다,
Streptomyces 1군 균주는 이제까지 리파이제를 생산하는 것으로 알려지지 않았다,
리파아제 제제는 pH 10에서 최적 활성의 50% 이상인 활성을 갖고 예를들어 세제에 유용하다,

Description

알칼리 리파아제 (ALKALINE LIPASE)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 내지 제7도는 다음 균주 S. griseus LB 501 (DSM 7349), S. griseus LB 502(DSM 7350), S. coelicolor LB 511 (FERM BP-4236), S. coelicolor LB 512 (FERM BP-4237), S. griseus LB 524(DSM 8672), S. coelicolor N 2293(ATCC 23899) 및 S. parvus N 2300 (ATCC 12433)으로부터 유래된 본 발명의 리파아제 제제의 활성에 대한 pH 프로파일을 보여준다. 구체적인 사항은 실시예 6에서 주어진다.

Claims (20)

1)위치 비특이적이고, 2) 40℃에서 20분동안 기질로서의 올리브유 및 유화제로서의 폴리비닐 알콜을 사용한 Ca++없는 분석에서 최적 pH 및 pH 10에서의 두 활성을 측정했을때, pH 10에서 최적 pH에서의 활성의 50% 이상인 활성을 가지며, 및 3)Streptomyces 1군 균주의 배양에 의해 생성된 수 있는 라파이제 제제.
제1항에 있어서, 균주는 S. coelicolor, S. limosus, S. alboviridis, S. griseus, S. parvus, S. setonii 또는 S. nitrosporeus인 것을 특징으로 하는 라파아제 제제.
제2항에 있어서, 균주는 S. coelicolor FERM BP-4236, FERM BP-4237, ATCC 23899, S. limosus ATCC 19778, S. alboviridis ATCC 25425, S. griseus ATCC 23345, DSM 7349, DSM 7350, DSM 8672, S. parvus ATCC 12433, S. setonii ATCC 25497 또는 S. nitrosporeus ATCC 27472인 것을 특징으로 하는 리파아제 제제.
제1항에 있어서, 60분 반응시간동안 기질로서의 올리브유 및 유화제로서의 폴리비닐 알콜을 사용하여 두활성을 측정하고, 세제용액이 선형 알킬 벤젠 설포네이트, 0.35g/ℓ, 알콜 에톡실레이트 0.15g/ℓ, 삼인산나트륨 1.25g/ℓ, 황산나트륨 1.00g/ℓ, 탄산나트륨 0.45g/ℓ 및 메타규산나트륨 0.15g/ℓ으로 구성될 때, pH 10.2인 세제용액에서 pH 10인 디에탄올 아민 완충액에서의 활성의 50%이상인 활성을 갖는 것을 특징으로 하는 리파아제 제제.
제4항에 있어서, 균주는 Streptomyces 아군 1A 또는 1B의 균주인 것을 특징으로 하는 리파아제 제제.
제5항에 있어서, 균주는 S. griseus, S. coelicolor 또는 S. parvus인 것을 특징으로 하는 라파아제 제제.
제6항에 있어서, 균주는 S. griseus DSM 7349, DSM 7350, DSM 8672, S. coelicolor FERM BP-4236, FERM BP-4237, ATCC 23899 또는 S. parvus ATCC 12433인 것을 특징으로 하는 리파아제 제제.
제4항에 있어서, pH 10에서 기질로서의 올리브유 및 유화제로서의 폴리비닐 알콜을 사용하여 두 활성을 측정했을 때 Ca++의 부재하에서 50mM Ca++존재하에서의 활성의 50%이상인 활성을 갖는 것을 특징으로 하는 리파아제 제제.
제8항에 있어서, 균주는 S. griseus DSM 7350인 것을 특징으로하는 리파아제 제제.
전항의 어느 한항에 있어서, 리파이제 제제가 비분말 과립, 안정화된 액체, 슬러리 또는 보호된 효소의 형태로 세제첨가제로서 제공되는 것을 특징으로 하는 리파아제 제제.
1)위치 비특이적이고, 2)40℃에서 20분도안 기질로서의 올리브유 및 유화제로서의 폴리비닐 알콜을 사용하여 측정했을때 pH 9-11의 범위에서 최적 활성을 가지며, 3)Streptomyces 1군 균주에 고유한 세포외의 리파아제와 면역학적으로 동일하거나 부분적으로 동일한 리파아제.
제6항의 리파아제 제제를 생성할 수 있는 Streptomyces griseus 균주.
제12항에 있어서, 상기 리파아제 제제를 생성할 수 있는 S. griseus DSM 7350또는 이들의 돌연변이체나 변이체인 것을 특징으로 하는 Streptomyces griseus 균주.
탄소원, 질소원 및 무기염을 포함하는 적당한 영양배지에서 리파아제 생성균주인 Streptomyces 1군 균주의 배양과 이후 리파아제 제제의 회수로 이루어지는 것을 특징으로 하는 제1항 내지 제10항중 어느 한항에 따른 리파아제 제제의 제조방법.
제14항에 있어서, 균주는 S. coelicolor, S. limosus, S. alboviridis, S. griseus, S. parvus, S. setonii 또는 S. nitrosporeus인 것을 특징으로 하는 방법.
제15항에 있어서, 균주는 S. coelicolor FERM BP-4236, FERM BP-4237, ATCC 23899, S. limosus ATCC 19778, S. alboviridis ATCC 25425, S. griseus ATCC 23345, DSM 7349, DSM 7350, DSM 8672, S. parvus ATCC 12433, S. setonii ATCC 25497 또는 S. nitrosporeus ATCC 27472 또는 이들의 리파이제-생성변이체이거나 돌연변이체인 것을 특징으로 하는 방법.
계면활성제와 제1항 내지 제17항중 어느 한항의 리파이제 제제로 이루어지는 세제 조성물.
제17항에 있어서, 세제빌더를 1∼40% 더 포함하고, 수용액에서 측정했을 때 pH7∼11을 나타내는 것을 특징으로하는 세제 조성물.
제18항에 있어서, 빌더가 인산염 빌더, 제올라이트 또는 시트르산 나트륨인 것을 특징으로 하는 세제 조성물.
제17항 내지 제19항중 어느 한항에 있어서, 프로테아제, 아밀라제, 셀룰라제, 옥시다제 및 퍼옥시다제로 구성되는 군에서 선택된 추가의 세제효소를 더 포함하는 것을 특징으로 하는 세제 조성물.
※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950702583A 1992-12-22 1993-12-22 알칼리 리파아제(alkaline lipase) KR950704467A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DK152992A DK152992D0 (da) 1992-12-22 1992-12-22 Nyt enzym
DK1529/92 1992-12-22
DK9693A DK9693D0 (da) 1993-01-28 1993-01-28 Nyt enzym
DK96/93 1993-01-28
DK442/93 1993-04-20
DK44293A DK44293D0 (da) 1993-04-20 1993-04-20 Nyt enzym
PCT/DK1993/000442 WO1994014940A1 (en) 1992-12-22 1993-12-22 Alkaline lipase

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KR950704467A true KR950704467A (ko) 1995-11-20

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US (1) US5763383A (ko)
EP (1) EP0675944B1 (ko)
JP (1) JP3507076B2 (ko)
KR (1) KR950704467A (ko)
AT (1) ATE243735T1 (ko)
BR (1) BR9307711A (ko)
CA (1) CA2152342A1 (ko)
DE (1) DE69333065T2 (ko)
FI (1) FI953080A (ko)
WO (1) WO1994014940A1 (ko)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69333065T2 (de) * 1992-12-22 2004-04-01 Novozymes A/S Alkalische lipase
AU3741995A (en) * 1994-10-26 1996-05-23 Novo Nordisk A/S Enzymatic detergent composition
US5919746A (en) * 1995-03-30 1999-07-06 Novo Nordisk A/S Alkaline lipolytic enzyme
WO1996030502A1 (en) * 1995-03-30 1996-10-03 Novo Nordisk A/S Alkaline lipolytic enzyme
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
DE19535082A1 (de) * 1995-09-21 1997-03-27 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
ATE279512T1 (de) 1996-04-25 2004-10-15 Novozymes As Alkalisches, lipolytisches enzym
US5821102A (en) * 1997-01-21 1998-10-13 Novo Nordisk Biotech Inc. Nucleic acids encoding polyeptides having absidia lipase activity
EP1679373A3 (en) * 1997-04-09 2008-01-23 Danisco A/S Lipase and use of same for improving doughs and baked products
ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
US7955814B2 (en) * 2003-01-17 2011-06-07 Danisco A/S Method
US20050196766A1 (en) * 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
GB0405637D0 (en) * 2004-03-12 2004-04-21 Danisco Protein
EP1791933B1 (en) * 2004-07-16 2011-06-29 Danisco A/S Enzymatic oil-degumming method
EP1876222A1 (en) * 2006-07-06 2008-01-09 Cognis IP Management GmbH Process for production of fatty acids, fatty acid esters and sterolesters from soapstock
BRPI0720801A2 (pt) 2007-01-25 2014-09-16 Dupont Nutrition Biosci Aps Produção de um lipídio aciltranfersase a partir de células transformadas de bacillus licheniformis.
WO2011078949A1 (en) 2009-12-21 2011-06-30 Danisco Us Inc. Surfactants that improve the cleaning of lipid-based stains treated with lipases
AR081423A1 (es) * 2010-05-28 2012-08-29 Danisco Us Inc Composiciones detergentes con contenido de lipasa de streptomyces griseus y metodos para utilizarlas
EP2395070A1 (en) * 2010-06-10 2011-12-14 The Procter & Gamble Company Liquid laundry detergent composition comprising lipase of bacterial origin
WO2014200656A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces umbrinus
WO2014200658A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from promicromonospora vindobonensis
WO2014200657A1 (en) 2013-06-13 2014-12-18 Danisco Us Inc. Alpha-amylase from streptomyces xiamenensis
EP3011020A1 (en) 2013-06-17 2016-04-27 Danisco US Inc. Alpha-amylase from bacillaceae family member
EP3052622B1 (en) 2013-10-03 2018-09-19 Danisco US Inc. Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
DK3060659T3 (da) 2013-10-03 2019-09-09 Danisco Us Inc Alfa-amylaser fra exiguobacterium og fremgangsmåder til anvendelse deraf
JP6560214B2 (ja) 2013-11-20 2019-08-14 ダニスコ・ユーエス・インク プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法
WO2016111884A2 (en) 2015-01-08 2016-07-14 Stepan Company Cold-water laundry detergents
WO2016160407A1 (en) 2015-03-31 2016-10-06 Stepan Company Detergents based on alpha-sulfonated fatty ester surfactants
WO2017173190A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2017173324A2 (en) 2016-04-01 2017-10-05 Danisco Us Inc. Alpha-amylases, compositions & methods
WO2023203080A1 (en) 2022-04-20 2023-10-26 Novozymes A/S Process for producing free fatty acids
WO2023209070A1 (en) 2022-04-29 2023-11-02 Novozymes A/S Production of fatty acid alkyl esters

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3431175A (en) * 1964-07-18 1969-03-04 Amano Pharma Co Ltd Method for the preparation of a lipoprotein lipase by cultivating organisms
US3317400A (en) * 1964-11-23 1967-05-02 Upjohn Co Enzymatic modification of bovine growth hormone by proteolytic streptomycete cell extracts
FI47779B (ko) * 1969-04-10 1973-11-30 Astra Laekemedel Ab
GB1428928A (en) * 1973-02-22 1976-03-24 Kanegafuchi Chemical Ind Producing l-lysine
JPS56134994A (en) * 1980-03-25 1981-10-22 Nippon Oil Co Ltd Separating and recovering method of porphyrin
DK399387D0 (da) * 1987-07-31 1987-07-31 Novo Industri As Immobiliseret lipase og dennes anvendelse
US5240851A (en) * 1988-02-22 1993-08-31 Fina Research, S.A. Lipase-producing Pseudomonas aeruginosa strain
JP2693786B2 (ja) * 1988-08-09 1997-12-24 ユニチカ株式会社 耐熱性のリポプロテインリパーゼとその製造法及びそれを用いた定量用試薬
FR2645771B1 (fr) * 1989-04-17 1991-06-14 Air Liquide Procede de restructuration d'un ensemble de poudres fines
DE69333065T2 (de) * 1992-12-22 2004-04-01 Novozymes A/S Alkalische lipase

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DE69333065T2 (de) 2004-04-01
EP0675944B1 (en) 2003-06-25
FI953080A0 (fi) 1995-06-21
BR9307711A (pt) 1999-09-08
JPH08504587A (ja) 1996-05-21
WO1994014940A1 (en) 1994-07-07
US5763383A (en) 1998-06-09
ATE243735T1 (de) 2003-07-15
FI953080A (fi) 1995-06-21
JP3507076B2 (ja) 2004-03-15
CA2152342A1 (en) 1994-07-07
EP0675944A1 (en) 1995-10-11
DE69333065D1 (de) 2003-07-31

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