KR950700418A - 신규 식물 유전자(novel plant gene) - Google Patents

신규 식물 유전자(novel plant gene)

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Publication number
KR950700418A
KR950700418A KR1019940703062A KR19940703062A KR950700418A KR 950700418 A KR950700418 A KR 950700418A KR 1019940703062 A KR1019940703062 A KR 1019940703062A KR 19940703062 A KR19940703062 A KR 19940703062A KR 950700418 A KR950700418 A KR 950700418A
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KR
South Korea
Prior art keywords
dna
plant
seq
nucleotide sequence
encoding
Prior art date
Application number
KR1019940703062A
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English (en)
Other versions
KR100245488B1 (ko
Inventor
야스히로 기꾸찌
시게또 기요까와
유끼히사 시마다
리쓰꼬 시마다
마사야 오바야시
야스시 오끼나까
Original Assignee
나까무라 간노스께
교오와학꼬고오교 가부시끼가이샤
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Publication of KR950700418A publication Critical patent/KR950700418A/ko
Application granted granted Critical
Publication of KR100245488B1 publication Critical patent/KR100245488B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/74—Rosaceae, e.g. strawberry, apple, almonds, pear, rose, blackberries or raspberries
    • A01H6/749—Rosa, i.e. roses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13088—Flavonoid 3',5'-hydroxylase (1.14.13.88)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은, 폴라보노이드-3′,5′-수산화효소 활성을 갖는 폴리펩티드를 코드하는 DNA, 이 DNA를 함유하는 재조합체 DNA 및 이 재조합체 DNA로 식물을 형질 전환함으로써 본래 갖고 있지 않은 색소 패턴을 갖는 식물에 관한 것이다.

Description

신규 식물 유전자(NOVEL PLANT GENE)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 공지의 시토크롬 P450 아미노산 배열의 80% 이상이 갖고 있는 코어배열이다.

Claims (11)

  1. 플라보노이브-3′,5′-수산화효소 활성을 가지며, 배열번호 1, 배열번호 63 또는 배열번호 64로 표시된 아미노산 배열로 나타나는 폴리펩티드를 코드하는 DNA 또는 이와 하이브리드화 하는 DNA.
  2. 제 1 항에 있어서, DNA의 일부의 염기 배열이 삭제 또는 다른 염기 배열로 치환된 DNA.
  3. 제1 또는 2항의 DNA를 백터 DNA에 조입하여서된 재조합체 DNA.
  4. 제 3 항의 재조합체 DNA를 보유하는 식물세포 또는 식물.
  5. 제 4 항에 있어서, 식물이 장미속, 니코티아나속, 페튜니어속 또는 술패랭이 꽃속에 속하는 식물인 식물세포 또는 식물.
  6. 50℃에서 2×SSC(0.3 M염화나트륨, 0.03M시트르산 나트륨, pH 7.0)중에서 배열번호 1, 배열번호 63 또는 배열번호 64로 표시된 염기 배열로 나타나는 DNA와 하이드브리화할 수 있는 DNA .
  7. 플라보노이드-3′,5′-수산화효소 활성을 갖는 폴리펩티드를 코드하는 DNA 단편과 벡터 DNA 단편으로써 재조합체 DNA를 식물에 보유시켜, 이 폴리펩티드를 코드하는 DNA의 유전 정보에 의한 색소를 발현하는 능력을 갖는, 식물을 육성시켜 얻어진 이 식물을 채취함을 특징으로 하는 식물의 제조법.
  8. 제 7 항에 있어서, 폴라보노이드-3′,5′-수산화효소 활성을 갖는 폴리펩티드를 코드하는 DNA 단편이 제 1 항 또는 제 2 항의 DNA인 식물의 제조법.
  9. 배열번호 2로부터 배열번호 29까지 표시한 염기배열을 갖는 DNA.
  10. 제 9 항의 DNA와 3′말단 8 염기의 배열이 일치하는 DNA.
  11. 제9 또는 10항의 DNA를 프라이머로 한 폴리머라아제 연쇄 반응 (PCR)법에 의하여, 시토크롬 P450효소의 헴결합 영역의 아미노산 배열을 코드하는 유전자 단편을 증폭단리하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703062A 1992-03-02 1992-11-20 신규 식물 유전자 KR100245488B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP92-44963 1992-03-02
JP44963/92 1992-03-02
JP4496392 1992-03-02
PCT/JP1992/001520 WO1993018155A1 (en) 1992-03-02 1992-11-20 Novel plant gene

Publications (2)

Publication Number Publication Date
KR950700418A true KR950700418A (ko) 1995-01-16
KR100245488B1 KR100245488B1 (ko) 2000-02-15

Family

ID=12706141

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940703062A KR100245488B1 (ko) 1992-03-02 1992-11-20 신규 식물 유전자

Country Status (7)

Country Link
US (3) US6114601A (ko)
EP (1) EP0632128A4 (ko)
JP (1) JP3403196B2 (ko)
KR (1) KR100245488B1 (ko)
AU (1) AU680401B2 (ko)
CA (1) CA2130800C (ko)
WO (1) WO1993018155A1 (ko)

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Also Published As

Publication number Publication date
CA2130800A1 (en) 1993-09-03
EP0632128A4 (en) 1996-04-24
US6114601A (en) 2000-09-05
AU680401B2 (en) 1997-07-31
KR100245488B1 (ko) 2000-02-15
EP0632128A1 (en) 1995-01-04
CA2130800C (en) 2002-05-14
WO1993018155A1 (en) 1993-09-16
AU2956092A (en) 1993-10-05
US6232109B1 (en) 2001-05-15
JP3403196B2 (ja) 2003-05-06
US20020100072A1 (en) 2002-07-25

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