KR950029353A - Novel Basophilic Protease Genes and Their Expression in Escherichia Coli - Google Patents

Novel Basophilic Protease Genes and Their Expression in Escherichia Coli Download PDF

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Publication number
KR950029353A
KR950029353A KR1019940009139A KR19940009139A KR950029353A KR 950029353 A KR950029353 A KR 950029353A KR 1019940009139 A KR1019940009139 A KR 1019940009139A KR 19940009139 A KR19940009139 A KR 19940009139A KR 950029353 A KR950029353 A KR 950029353A
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South Korea
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basophilic
gene
expression vector
coli
expression
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KR1019940009139A
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Korean (ko)
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KR970009083B1 (en
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유욱준
이황희
장원희
김은경
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심상철
한국과학기술원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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Abstract

본 발명은 비브리오속으로 부터 유래된 호염기성 단백질 분해 효소를 코딩하는 유전자, 상기 유전자를 포함하는 발현벡터, 상기 발현벡터로 형질전환된 대장균 및 상기 대장균을 배양함을 포함하는 호염기성 단백질 분해효소의 생산방법을 제공한다.The present invention provides a gene encoding a basophilic proteolytic enzyme derived from Vibrio genus, an expression vector comprising the gene, Escherichia coli transformed with the expression vector, and a basophilic protease comprising culturing the E. coli. Provide production methods.

Description

신규한 호염기성 단백질 분해효소 유전자 및 대장균에서의 그의 발현Novel Basophilic Protease Genes and Their Expression in Escherichia Coli

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따른 호염기성 단백질 분해 효소의 뉴클레오티드서열 및 아미노산 서열을 나타낸 것이고, 제2도는 본 발명에 따른 재조합 플라스미드의 제조과정을 나타낸 것이며, 제3도는 호염기성 단백질 분해효소 활성을 갖는 슈도모나스종 균주, pUC벡터를 함유하는 대장균(E. coli) JM 83 및 플라스미드 pAP 101을 함유하는 대장균 JM 83에서 호염기성 단백질 분해 효소를 발현시킨 결과를 나타낸 것이고, 제4도는 플라스미드 pAP 101을 함유하는 대장균 JM 83에서의 호염기성 단백질 분해효소의 발현을 SDS-PAGE로 확인한 결과를 나타낸 것이며, 제5도는 플라스미드 pAP 101을 함유하는 대장균 JM 83으로부터 생성된 단백질 분해효소의 활성 측정결과를 나타낸 것이다.Figure 1 shows the nucleotide sequence and amino acid sequence of the basophilic proteolytic enzyme according to the present invention, Figure 2 shows the manufacturing process of the recombinant plasmid according to the present invention, Figure 3 is Pseudomonas having basophilic protease activity Species strain, E. coli JM 83 containing the pUC vector and E. coli JM 83 containing the plasmid pAP 101 were shown to express basophilic proteolytic enzyme, Figure 4 is E. coli containing plasmid pAP 101 Expression of basophilic protease in JM 83 was confirmed by SDS-PAGE, and FIG. 5 shows the result of measuring activity of protease produced from E. coli JM 83 containing plasmid pAP 101.

Claims (8)

슈도모나스종에 속하는 균주로 부터 유래된 호염기성 단백질 분해 효소 단백질을 코딩하는 유전자.A gene encoding a basolytic proteolytic enzyme protein derived from a strain belonging to Pseudomonas species. 제1항에 있어서, 하기 뉴클레오티드 서열을 갖는 유전자.The gene of claim 1 having the following nucleotide sequence. 제2항에 유전자에 의해 코팅되는, 하기 아미노산 서열을 갖는 호염기성 단백질 분해효소.Basophil protease having the following amino acid sequence, coated by the gene of claim 2. 제1항 또는 제2항의 유전자를 포함하는 발현벡터.An expression vector comprising the gene of claim 1 or 2. 제4항에 있어서, 발현벡터 pAP 101 (KFCC-10817)The expression vector pAP 101 (KFCC-10817) according to claim 4 제4항 또는 제5항의 발현 벡터로 형질 전환된 대장균.E. coli transformed with the expression vector of claim 4 or 5. 제6항에 있어서, 대장균 JM 83/pAP 101 (KFCC-10817)E. coli JM 83 / pAP 101 (KFCC-10817) 제6항 또는 제7항의 대장균을 배양함을 포함하는, 호염기성 단백질 분해 효소 단백질의 제조방법.A method for producing a basolytic proteolytic enzyme protein, comprising culturing E. coli according to claim 6. ※ 참고사항 : 최초출원 내용에 의하여 공개 하는 것임.※ Note: The disclosure is based on the initial application.
KR94009139A 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli KR970009083B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

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KR950029353A true KR950029353A (en) 1995-11-22
KR970009083B1 KR970009083B1 (en) 1997-06-05

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KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110086711A (en) * 2008-10-21 2011-07-29 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 Process for producing protein capable of forming inclusion body
KR101137020B1 (en) * 2006-12-15 2012-04-19 한국생명공학연구원 A cellulase gene from thermophilic microorganism

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101137020B1 (en) * 2006-12-15 2012-04-19 한국생명공학연구원 A cellulase gene from thermophilic microorganism
KR20110086711A (en) * 2008-10-21 2011-07-29 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 Process for producing protein capable of forming inclusion body

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