KR970009083B1 - Novel alkaline protease gene and expression thereof in e.coli - Google Patents

Novel alkaline protease gene and expression thereof in e.coli Download PDF

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Publication number
KR970009083B1
KR970009083B1 KR94009139A KR19940009139A KR970009083B1 KR 970009083 B1 KR970009083 B1 KR 970009083B1 KR 94009139 A KR94009139 A KR 94009139A KR 19940009139 A KR19940009139 A KR 19940009139A KR 970009083 B1 KR970009083 B1 KR 970009083B1
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KR
South Korea
Prior art keywords
coli
expression
alkaline protease
protease gene
novel alkaline
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KR94009139A
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Korean (ko)
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KR950029353A (en
Inventor
Wook-Joon Yu
Hwang-Hee Lee
Won-Hee Jang
Eun-Kyung Kim
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Korea Advanced Inst Sci & Tech
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Priority to KR94009139A priority Critical patent/KR970009083B1/en
Publication of KR950029353A publication Critical patent/KR950029353A/en
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Publication of KR970009083B1 publication Critical patent/KR970009083B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Basophilic proteinase gene is cloned from an alkali-tolerant strain which belongs to Pseudomonas sp. Proteinase is produced by the cultivation of E. coli (JM83/pAP101 KFCC-10817), which is transformed by expression vector (pAP101 KFCC-10817) including above described proteinase gene.
KR94009139A 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli KR970009083B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

Publications (2)

Publication Number Publication Date
KR950029353A KR950029353A (en) 1995-11-22
KR970009083B1 true KR970009083B1 (en) 1997-06-05

Family

ID=19381987

Family Applications (1)

Application Number Title Priority Date Filing Date
KR94009139A KR970009083B1 (en) 1994-04-28 1994-04-28 Novel alkaline protease gene and expression thereof in e.coli

Country Status (1)

Country Link
KR (1) KR970009083B1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101137020B1 (en) * 2006-12-15 2012-04-19 한국생명공학연구원 A cellulase gene from thermophilic microorganism
WO2010047347A1 (en) * 2008-10-21 2010-04-29 財団法人化学及血清療法研究所 Process for producing protein capable of forming inclusion body

Also Published As

Publication number Publication date
KR950029353A (en) 1995-11-22

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