KR930013107A - 인간 알파인터페론 유전자의 발현벡터 - Google Patents

인간 알파인터페론 유전자의 발현벡터 Download PDF

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Publication number
KR930013107A
KR930013107A KR1019910025878A KR910025878A KR930013107A KR 930013107 A KR930013107 A KR 930013107A KR 1019910025878 A KR1019910025878 A KR 1019910025878A KR 910025878 A KR910025878 A KR 910025878A KR 930013107 A KR930013107 A KR 930013107A
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South Korea
Prior art keywords
alpha interferon
human alpha
expression
interferon gene
yeast
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KR1019910025878A
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English (en)
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KR930008976B1 (ko
Inventor
조중명
박영우
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최근선
주식회사 럭키
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 최근선, 주식회사 럭키 filed Critical 최근선
Priority to KR1019910025878A priority Critical patent/KR930008976B1/ko
Priority to MYPI92002359A priority patent/MY108667A/en
Priority to AU30371/92A priority patent/AU648214B2/en
Priority to AT92122084T priority patent/ATE206457T1/de
Priority to EP01101958A priority patent/EP1097995A1/en
Priority to DE69232094T priority patent/DE69232094T2/de
Priority to EP92122084A priority patent/EP0553494B1/en
Priority to ES92122084T priority patent/ES2165352T3/es
Priority to CN92111826A priority patent/CN1062014C/zh
Priority to TW082100318A priority patent/TW242653B/zh
Publication of KR930013107A publication Critical patent/KR930013107A/ko
Application granted granted Critical
Publication of KR930008976B1 publication Critical patent/KR930008976B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

인간 알파인터페론 유전자의 발현벡터
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 프로모터 AG885와 알파인터페론 유전자의 5'말단 일부 염기서열을 나타낸 것이고,
제 2 도는 효모발현 벡터로의 클로닝 과정을 도시화한 것이며,
제 3 도는 인간 알파인터페론 유전자가 함유된 재조합 효모에서 알파인터페론 단백질이 대량 발현된 것을 확인한 SDS-폴리아크릴아미드 젤 전기영동 결과를 나타낸 것이다.

Claims (3)

  1. 효모용 인간 알파인터페론의 발현벡터 pYLBC-AG885-α-IFN.
  2. 제 1 항에 의해 형질전환된 효모 사카로마이세스 세레비지애(Saccharomyces cerevisiae)(pYLBC-AG885-α-IFN )(KCTC 0028 BP)
  3. 제2항의 효모를 이용한 인간 알파인터페론의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019910025878A 1991-12-31 1991-12-31 인간 알파인터페론 유전자의 발현벡터 KR930008976B1 (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
KR1019910025878A KR930008976B1 (ko) 1991-12-31 1991-12-31 인간 알파인터페론 유전자의 발현벡터
MYPI92002359A MY108667A (en) 1991-12-31 1992-12-21 Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
AU30371/92A AU648214B2 (en) 1991-12-31 1992-12-22 Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
EP01101958A EP1097995A1 (en) 1991-12-31 1992-12-29 Process for purification of human alpha interferon produced in yeast cells
AT92122084T ATE206457T1 (de) 1991-12-31 1992-12-29 Rekombinanter gen kodierend für alpha interferon und expressionsvector dafür
DE69232094T DE69232094T2 (de) 1991-12-31 1992-12-29 Rekombinanter Gen kodierend für Alpha Interferon und Expressionsvector dafür
EP92122084A EP0553494B1 (en) 1991-12-31 1992-12-29 Recombinant gene coding for human alpha interferon and expression vector thereof, ETC.
ES92122084T ES2165352T3 (es) 1991-12-31 1992-12-29 Gen recombinante que codifica el interferon alfa humano y vector de expresion del mismo.
CN92111826A CN1062014C (zh) 1991-12-31 1992-12-31 编码人α－干扰素的重组基因、表达载体的制备方法及该干扰素的纯化
TW082100318A TW242653B (ko) 1991-12-31 1993-01-19

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910025878A KR930008976B1 (ko) 1991-12-31 1991-12-31 인간 알파인터페론 유전자의 발현벡터

Publications (2)

Publication Number Publication Date
KR930013107A true KR930013107A (ko) 1993-07-21
KR930008976B1 KR930008976B1 (ko) 1993-09-17

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Application Number Title Priority Date Filing Date
KR1019910025878A KR930008976B1 (ko) 1991-12-31 1991-12-31 인간 알파인터페론 유전자의 발현벡터

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990010720A (ko) * 1997-07-18 1999-02-18 구광시 효모용 인간 알파인터페론 유전자의 발현벡터, 이를 이용하여 형질전환된 효모 및 이를 이용한 인간 알파인터페론의 제조방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990010720A (ko) * 1997-07-18 1999-02-18 구광시 효모용 인간 알파인터페론 유전자의 발현벡터, 이를 이용하여 형질전환된 효모 및 이를 이용한 인간 알파인터페론의 제조방법

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Publication number Publication date
KR930008976B1 (ko) 1993-09-17

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