KR920703788A - 세포 배양 방법 - Google Patents

세포 배양 방법

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Publication number
KR920703788A
KR920703788A KR1019920701825A KR920701825A KR920703788A KR 920703788 A KR920703788 A KR 920703788A KR 1019920701825 A KR1019920701825 A KR 1019920701825A KR 920701825 A KR920701825 A KR 920701825A KR 920703788 A KR920703788 A KR 920703788A
Authority
KR
South Korea
Prior art keywords
bubbles
medium
mol
bubble
gas
Prior art date
Application number
KR1019920701825A
Other languages
English (en)
Inventor
이사벨라 마리아 레오나르다 욥세스
게라르두스 코르넬리스 후겐두른
요한네스 마르가레타 마리아 우멘스
Original Assignee
에프 지 엠 헤르만스,에이.지.제이.베르미렌
악조 엔. 브이
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에프 지 엠 헤르만스,에이.지.제이.베르미렌, 악조 엔. 브이 filed Critical 에프 지 엠 헤르만스,에이.지.제이.베르미렌
Publication of KR920703788A publication Critical patent/KR920703788A/ko

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

세포 배양 방법
[도면의 간단한 설명]
제1도에는, 포화기(1)을 통해 기체상 영양물로 포화된 배지를 위한 유입구(3)가 장착된 반응 용기(4)가 제시되어 있다.
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (6)

  1. 적어도 부분적으로 배지로 충진된 생반응기내의 세포에 기체상 영양물을 기포의 형태로 배송하여 공급하는 방법으로서, 기포의 체류 시간이 기포가 배지중에 용해되는 시간과 대등함을 특징으로 하는 방법.
  2. 제1항에 있어서, 배지가 비-교반되며, 기포는 생반응기내의 배지 컬럼 최대 높이의 80-95%에서 용해됨을 특징으로 하는 방법.
  3. 제2항에 있어서, 기포의 초기 직경(db)이
    (여기에서, D=확산 계수[m2/s], △C=매스 이동에 대한 추진력 즉, 기포 근방의 기체상 성분 농도와 벌크 농도간의 차이[mol/m3], η=점도[Pa. s], △ρ=배지의 밀도-기체 기포의 밀도[kg/m3], Cmol=기체 몰 농도[mole/m3] 및 Hr=반응기내 액체 컬럼의 높이[m]에 부합하는 것을 특징으로 하는 방법.
  4. 제2항 또는 3항에 있어서, 액체 컬럼의 높이가 1미터이고 기포의 초기 직경은 250μm과 대등하거나 250μm미만임을 특징으로 하는 방법.
  5. 제1항에 있어서, 배지가 교반되고, 초기 기포 직경이
    (여기에서, KL=매스 이동 계수[m/s], △C=매스 이동에 대한 추진력 즉, 기포 근방의 기체상 성분 농도와 벌크 농도간의 차이[mol/m3], Cmol=기체 몰 농도[mole/m2] 및 tr=체류 시간)에 부합하는 것을 특징으로 하는 방법.
  6. 제5항에 있어서, 기포의 초기 직경이 300μm와 대등하거나 300μm미만임을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920701825A 1990-02-01 1991-01-29 세포 배양 방법 KR920703788A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP90200243 1990-02-01
EP90200243.5 1990-02-01
PCT/EP1991/000001 WO1991011508A1 (en) 1990-02-01 1991-01-29 Method for culturing cells

Publications (1)

Publication Number Publication Date
KR920703788A true KR920703788A (ko) 1992-12-18

Family

ID=8204928

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019920701825A KR920703788A (ko) 1990-02-01 1991-01-29 세포 배양 방법

Country Status (7)

Country Link
EP (1) EP0513057A1 (ko)
JP (1) JPH05503848A (ko)
KR (1) KR920703788A (ko)
AU (1) AU651080B2 (ko)
CA (1) CA2075059A1 (ko)
FI (1) FI922409A0 (ko)
WO (1) WO1991011508A1 (ko)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US6949244B1 (en) 1995-12-20 2005-09-27 The Board Of Trustees Of The University Of Kentucky Murine monoclonal anti-idiotype antibody 11D10 and methods of use thereof
DE102008064279A1 (de) * 2008-12-20 2010-06-24 Bayer Technology Services Gmbh Bioreaktor
JP2011078371A (ja) * 2009-10-09 2011-04-21 Tomotaka Marui 細胞変化を促進する微小気泡含有組成物、およびその微小気泡含有組成物を製造する装置、ならびに微小気泡含有組成物を用いた細胞変化促進方法。
JP5549209B2 (ja) * 2009-12-11 2014-07-16 株式会社Ｉｈｉ 付着性細胞培養装置
JP2014183742A (ja) * 2013-03-21 2014-10-02 Yamagata Univ ポリ塩化ビフェニル類無害化複合組成物ならびにその製造方法
WO2016079820A1 (ja) * 2014-11-19 2016-05-26 三菱化学エンジニアリング株式会社 生物反応装置、生物反応方法、並びに、生物反応装置において使用される好気性微生物を担持させた多孔質構造体及びこの多孔質構造体の製造方法
WO2017017830A1 (ja) * 2015-07-30 2017-02-02 三菱化学エンジニアリング株式会社 酸素富化マイクロナノバブルを用いた生物反応装置及びこの生物反応装置を用いた生物反応方法
US11719704B2 (en) 2015-12-30 2023-08-08 Momenta Pharmaceuticals, Inc. Methods related to biologics
JP6138390B1 (ja) * 2017-01-25 2017-05-31 三菱化学エンジニアリング株式会社 マイクロナノバブルを用いた生物反応装置およびこの生物反応装置を用いた生物反応方法
KR102476633B1 (ko) 2018-06-27 2022-12-09 후지필름 가부시키가이샤 세포 배양 방법, 생산물의 제조 방법, 및 세포 배양 장치
CN113286650A (zh) * 2018-09-06 2021-08-20 动量制药公司 连续细胞培养方法
CN116606713B (zh) * 2023-07-12 2024-03-15 无锡海拓环保装备科技有限公司 一种菌群培养装置

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3968035A (en) * 1973-04-05 1976-07-06 Eli Lilly And Company Super-oxygenation method
US4654305A (en) * 1981-08-25 1987-03-31 The Board Of Governors For Higher Education State Of Rhode Island And Providence Plantations Multiphase reactor systems based on foams for simultaneous growth and separation of products

Also Published As

Publication number Publication date
AU651080B2 (en) 1994-07-14
EP0513057A1 (en) 1992-11-19
WO1991011508A1 (en) 1991-08-08
CA2075059A1 (en) 1991-08-02
JPH05503848A (ja) 1993-06-24
FI922409A (fi) 1992-05-26
AU7078791A (en) 1991-08-21
FI922409A0 (fi) 1992-05-26

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