KR920703111A - 테크네튬으로 1가의 항체단편을 빠르게 방사성라벨링하는 방법 - Google Patents

테크네튬으로 1가의 항체단편을 빠르게 방사성라벨링하는 방법

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Publication number
KR920703111A
KR920703111A KR1019920700598A KR920700598A KR920703111A KR 920703111 A KR920703111 A KR 920703111A KR 1019920700598 A KR1019920700598 A KR 1019920700598A KR 920700598 A KR920700598 A KR 920700598A KR 920703111 A KR920703111 A KR 920703111A
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Prior art keywords
antibody fragment
monovalent antibody
antibody
sterile
monovalent
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KR1019920700598A
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English (en)
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KR960015611B1 (ko
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제이 한센 한스
렌타인-존슨 아나스타시아
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원본미기재
임뮤노메딕스 인코포레이티드
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Publication of KR920703111A publication Critical patent/KR920703111A/ko
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Publication of KR960015611B1 publication Critical patent/KR960015611B1/ko

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
    • A61K51/1045—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants
    • A61K51/1048—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody against animal or human tumor cells or tumor cell determinants the tumor cell determinant being a carcino embryonic antigen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
    • A61K51/1282—Devices used in vivo and carrying the radioactive therapeutic or diagnostic agent, therapeutic or in vivo diagnostic kits, stents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2123/00—Preparations for testing in vivo

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Dispersion Chemistry (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)

Abstract

내용 없음

Description

테크네튬으로 1가의 항체단편을 빠르게 방사성라벨링하는 방법
[도면의 간단한 설명]
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. (1A) pH4.5-5.0의, 시염수를 함유하는 약 0.04-0.06M 아세테이트 완충액에 최소한 하나의 유리 실프하이드릴기, 항체단편의 mg당 약 10-150μgSn의 양으로의 염화제1주석, 및 약 30-40-폴드그램분자량 초파의 염화제1주석 이상의 타르트레이트를 갖는 1가 항체단편의 섬광영상화를 위한 단위투여량을 함유하는 무균용액, 또는 (1B)pH 4.5-5.0의 시염수를 함유하고 슈크로오즈에서 약 0.08-0.1M로 만들어진 약 0.04-0.06M 아세테이트 완충액에 최소한 하나의 유리 설프하이드릴기, 항체단편의 mg당 약 10-150μgSn의 양으로의 염화 제1주석, 약 30-40-폴드그램분자량 초과의 염화제1주석이상의 타르트레이트를 갓는 1가 항체단편의 성광영상화를 위한 단위투여량을 함유하는 무균용액의 동결건조물과, (2)효과적인 섬광영상화양이 Tc-997m-과테크네뮴산염을 함유하는 무균용액을 혼합하는 단계를 포함하므로서 Tc-99m으로 항체단편의 실제적이고도 정량적인 라벨링을 상온에서 약 5분에 달성할 수 있으며 Tc-997m-라벨링 1가 항체단편의 무균용액이 방사성 면역검출을 위한 환자에게 즉시 주입할 수 있게 함을 특징으로 하는 Tc-99m-라벨링 1가 항체단편의 무균의 주입가능용액 제조방법.
  2. 제1항에 있어서, 상기 1가 항체단편이 Fab-SH 또는 Fab'-SH 단편임을 특징으로 하는 방법.
  3. 제1항에 있어서, 상기 항체 또는 항체단편이 종양반(tumor marker)을 특이적으로 결합하는 것을 특징으로 하는 방법.
  4. 제1항에 있어서, 상기 항체 또는 항체단편이 감염병해, 미생뭍 또는 기생충과 회합된 항원을 특이적으로 결합하는 것을 특징으로 하는 방법.
  5. 제1항에 있어서, 상기 항체 또는 항체단편이 심근경색증, 응집물 또는 동맥경화증 반파 화합된 항원을 특이적으로 결합하는 것을 특징으로 하는 방법.
  6. 제1항에 있어서, 상기 항체 또는 항체단편이 정상기관 또는 조직과 회합된 항원을 특이적으로 결합하는 것을 특징으로 하는 방법.
  7. pH4.5-5.0의, 식염수를 함유하는 약 0.04-0.06M 아세레이트 원충액에 최소한 하나의 실프하이드릴기, 항체단편의 mg당 약 10-150μgSn의 양으로의 염화제1주석 및 30-40-폴드그램분자량과의 염화제1주석 이상의 타트트레이트를 갖는 1가 항체단편의 섬광영상화를 위한 단위 투여량을 기본적으로 함유하는 무균용액이 넣어진 밀봉된 무균의 용기를 포함하는데 항체단편이 종양, 감염명해, 미생물, 기생충, 심근경색증, 응집물, 동매기경화증 반 또는 정상기관 또는 조직에 의해 생성되거나 그들과 회합된 항원에 특이적으로 결합하는 것을 특징으로 하는 Tc-99m으로 1가 항체단편을 방사성 라벨링하기 위한 킷트.
  8. pH4.5-5.0의, 식염수를 함유하고 슈크로오즈에서 약 0.08-0.1M로 만들어진 약 0.04-0.06M 아세테이트 완충액에 최소한 하나의 유리 설프하이드릴기, 항체단편의 mg당 약 10-150μgSn의 양으로의 염화제1주석, 약 30-40-폴드 그램분자량 초과의 염화제1주석 이상의 타르트레이트를 갖는 1가 항체단편의 섬광영상화를 위한 단위투여량을 기본적으로 함유하는 무균용액의 동결 건조물이 넣어진 밀봉된 무균용기를 포함하는데 항체단편이 종양, 감염병해, 미생물, 기생충, 심근경색증, 응집물, 동맥경화중 반 또는 정상기관 또는 조직에 의해 생성되거나 그들과 회합된 항원에 특이적으로 결합하는 것을 특징으로 하는 Tc-99m으로 1가 항체단편을 방사성라벨링하기위한 킷트.
    ※ 참고사항:최초출원 내용에 의하여 공개하는 것임.
KR1019920700598A 1989-09-18 1990-09-18 테크네튬으로 1가의 항체단편을 빠르게 방사성 라벨링하는 방법 KR960015611B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40824189A 1989-09-18 1989-09-18
US408,241 1989-09-18
PCT/US1990/005196 WO1991004056A1 (en) 1989-09-18 1990-09-18 Method for rapidly radiolabeling monovalent antibody fragments with technetium

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KR920703111A true KR920703111A (ko) 1992-12-17
KR960015611B1 KR960015611B1 (ko) 1996-11-18

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KR1019920700598A KR960015611B1 (ko) 1989-09-18 1990-09-18 테크네튬으로 1가의 항체단편을 빠르게 방사성 라벨링하는 방법

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Country Link
EP (1) EP0419203B1 (ko)
JP (1) JPH0723326B2 (ko)
KR (1) KR960015611B1 (ko)
AT (1) ATE110281T1 (ko)
AU (1) AU640698B2 (ko)
CA (1) CA1340250C (ko)
DE (1) DE69011789T2 (ko)
DK (1) DK0419203T3 (ko)
ES (1) ES2063286T3 (ko)
FI (1) FI921146A (ko)
IE (1) IE64399B1 (ko)
IL (1) IL95723A (ko)
NO (1) NO920853L (ko)
WO (1) WO1991004056A1 (ko)
ZA (1) ZA907420B (ko)

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Publication number Publication date
NO920853D0 (no) 1992-03-04
EP0419203B1 (en) 1994-08-24
FI921146A0 (fi) 1992-03-17
DE69011789D1 (de) 1994-09-29
IE64399B1 (en) 1995-08-09
DK0419203T3 (da) 1994-12-05
IL95723A (en) 1995-08-31
DE69011789T2 (de) 1994-12-15
IE903360A1 (en) 1991-04-10
IL95723A0 (en) 1991-06-30
KR960015611B1 (ko) 1996-11-18
CA1340250C (en) 1998-12-15
EP0419203A1 (en) 1991-03-27
FI921146A (fi) 1992-03-17
AU640698B2 (en) 1993-09-02
WO1991004056A1 (en) 1991-04-04
JPH04505455A (ja) 1992-09-24
NO920853L (no) 1992-03-04
JPH0723326B2 (ja) 1995-03-15
ZA907420B (en) 1991-07-31
ES2063286T3 (es) 1995-01-01
AU6521490A (en) 1991-04-18
ATE110281T1 (de) 1994-09-15

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