KR920701463A - 세포를 성장시키기 위한 방법 조성물 및 장치 - Google Patents
세포를 성장시키기 위한 방법 조성물 및 장치Info
- Publication number
- KR920701463A KR920701463A KR1019910700181A KR910700181A KR920701463A KR 920701463 A KR920701463 A KR 920701463A KR 1019910700181 A KR1019910700181 A KR 1019910700181A KR 910700181 A KR910700181 A KR 910700181A KR 920701463 A KR920701463 A KR 920701463A
- Authority
- KR
- South Korea
- Prior art keywords
- cells
- culture medium
- human progenitor
- progenitor
- growing cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5403—IL-3
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5412—IL-6
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0641—Erythrocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0642—Granulocytes, e.g. basopils, eosinophils, neutrophils, mast cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/14—Erythropoietin [EPO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1394—Bone marrow stromal cells; whole marrow
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Developmental Biology & Embryology (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019980708792A KR100201662B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
KR1019980708793A KR100201663B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36663989A | 1989-06-15 | 1989-06-15 | |
US366,639 | 1989-06-15 | ||
PCT/US1990/003438 WO1990015877A2 (en) | 1989-06-15 | 1990-06-14 | Methods, compositions and devices for growing cells |
Publications (2)
Publication Number | Publication Date |
---|---|
KR920701463A true KR920701463A (ko) | 1992-08-11 |
KR100196062B1 KR100196062B1 (ko) | 1999-06-15 |
Family
ID=23443871
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019910700181A KR100196062B1 (ko) | 1989-06-15 | 1990-06-14 | 세포를 성장시키기 위한 방법, 조성물 및 장치 |
KR1019980708792A KR100201662B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
KR1019980708793A KR100201663B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019980708792A KR100201662B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
KR1019980708793A KR100201663B1 (en) | 1989-06-15 | 1998-10-28 | Methods, compositions and devices for growing cells |
Country Status (9)
Country | Link |
---|---|
EP (2) | EP0477290B1 (ko) |
JP (1) | JPH04506153A (ko) |
KR (3) | KR100196062B1 (ko) |
AT (1) | ATE148502T1 (ko) |
CA (1) | CA2062741A1 (ko) |
DE (1) | DE69029856T2 (ko) |
DK (1) | DK0477290T3 (ko) |
ES (1) | ES2097149T3 (ko) |
WO (1) | WO1990015877A2 (ko) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5182111A (en) * | 1987-11-17 | 1993-01-26 | Boston University Research Foundation | In vivo delivery of active factors by co-cultured cell implants |
US5763266A (en) * | 1989-06-15 | 1998-06-09 | The Regents Of The University Of Michigan | Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells |
US5437994A (en) * | 1989-06-15 | 1995-08-01 | Regents Of The University Of Michigan | Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells |
US5399493A (en) * | 1989-06-15 | 1995-03-21 | The Regents Of The University Of Michigan | Methods and compositions for the optimization of human hematopoietic progenitor cell cultures |
US5605822A (en) * | 1989-06-15 | 1997-02-25 | The Regents Of The University Of Michigan | Methods, compositions and devices for growing human hematopoietic cells |
US5635386A (en) | 1989-06-15 | 1997-06-03 | The Regents Of The University Of Michigan | Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture |
US5635387A (en) * | 1990-04-23 | 1997-06-03 | Cellpro, Inc. | Methods and device for culturing human hematopoietic cells and their precursors |
ATE225847T1 (de) * | 1992-03-04 | 2002-10-15 | Univ Michigan | Verfahren,zusammensetzungen und vorrichtungen zur erhaltung und zur züchtung menschlicher stammzellen und/oder hämatopoetischer zellen |
US6037174A (en) * | 1993-08-23 | 2000-03-14 | Nexell Therapeutics, Inc. | Preparation of serum-free suspensions of human hematopoietic cells or precursor cells |
US5846529A (en) * | 1993-08-23 | 1998-12-08 | Nexell Therapeutics, Inc. | Infusion of neutrophil precursors for treatment of neutropenia |
US5512480A (en) * | 1994-03-11 | 1996-04-30 | Baxter International Inc. | Flow-through bioreactor with grooves for cell retention |
DE19537774C2 (de) * | 1994-11-18 | 1996-09-19 | Heraeus Instr Gmbh | Verfahren zur Zellkultivierung und Vorrichtung zur Durchführung des Verfahrens |
DE19504958C1 (de) * | 1995-02-15 | 1995-11-02 | Heraeus Instr Gmbh | Verfahren für die Kultivierung von Zellen auf einem Träger, Vorrichtung zur Durchführung des Verfahrens und Verwendung der Vorrichtung |
US5688687A (en) * | 1995-06-07 | 1997-11-18 | Aastrom Biosciences, Inc. | Bioreactor for mammalian cell growth and maintenance |
US5728581A (en) * | 1995-06-07 | 1998-03-17 | Systemix, Inc. | Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein |
EP0870012B1 (en) * | 1995-12-29 | 2001-11-14 | Alg Company | Expansion of bone marrow stromal cells |
US6440734B1 (en) | 1998-09-25 | 2002-08-27 | Cytomatrix, Llc | Methods and devices for the long-term culture of hematopoietic progenitor cells |
US20020034819A1 (en) | 1998-02-23 | 2002-03-21 | Alan K. Smith | Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses |
US6548299B1 (en) | 1999-11-12 | 2003-04-15 | Mark J. Pykett | Lymphoid tissue-specific cell production from hematopoietic progenitor cells in three-dimensional devices |
JP2002529073A (ja) * | 1998-11-12 | 2002-09-10 | セル サイエンス セラピューティックス インコーポレーテッド | 三次元装置における造血前駆細胞からのリンパ様組織特異的細胞の生成 |
AU784220B2 (en) | 1999-08-24 | 2006-02-23 | Una Chen-Bettecken | Method for growing stem cells |
JP2004504834A (ja) | 2000-08-01 | 2004-02-19 | イスム リサーチ ディベロップメント カンパニー | 胚性細胞の定方向分化 |
DE10115098A1 (de) * | 2001-03-27 | 2002-10-02 | G & K Umformtechnik Gmbh | Vorrichtung zum Stanzen |
BR0309131A (pt) * | 2002-04-08 | 2005-02-01 | Millenium Biologix Inc | Sistema de criação de tecidos automatizado |
KR100980849B1 (ko) | 2007-04-25 | 2010-09-10 | 가톨릭대학교 산학협력단 | 인터루킨(il)-10을 포함하는 조혈모세포의 자가 재생산을촉진하는 조성물 |
JP2011045269A (ja) * | 2009-08-26 | 2011-03-10 | Hirosaki Univ | 幹細胞および/または前駆細胞の培養方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4135975A (en) * | 1977-12-14 | 1979-01-23 | Lichtman Marshall A | Obtaining human cell lines that elaborate colony stimulating activity for marrow cells of man and other species and methods of preparing same |
US4514499A (en) * | 1983-02-04 | 1985-04-30 | Corning Glass Works | Cell culture using a monolithic support |
US4847201A (en) * | 1985-02-05 | 1989-07-11 | Cetus Corporation | DNA encoding for CSF-1 and accompanying recombinant systems |
US4861714A (en) * | 1985-04-04 | 1989-08-29 | Verax Corporation | Weighted collagen microsponge for immobilizing bioactive material |
US4810643A (en) * | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
US4889812A (en) * | 1986-05-12 | 1989-12-26 | C. D. Medical, Inc. | Bioreactor apparatus |
-
1990
- 1990-06-14 WO PCT/US1990/003438 patent/WO1990015877A2/en active IP Right Grant
- 1990-06-14 DE DE69029856T patent/DE69029856T2/de not_active Expired - Lifetime
- 1990-06-14 ES ES90910240T patent/ES2097149T3/es not_active Expired - Lifetime
- 1990-06-14 DK DK90910240.2T patent/DK0477290T3/da active
- 1990-06-14 JP JP2509575A patent/JPH04506153A/ja active Pending
- 1990-06-14 AT AT90910240T patent/ATE148502T1/de not_active IP Right Cessation
- 1990-06-14 EP EP90910240A patent/EP0477290B1/en not_active Expired - Lifetime
- 1990-06-14 EP EP96201874A patent/EP0753574A1/en not_active Ceased
- 1990-06-14 CA CA002062741A patent/CA2062741A1/en not_active Abandoned
- 1990-06-14 KR KR1019910700181A patent/KR100196062B1/ko not_active IP Right Cessation
-
1998
- 1998-10-28 KR KR1019980708792A patent/KR100201662B1/ko not_active IP Right Cessation
- 1998-10-28 KR KR1019980708793A patent/KR100201663B1/ko not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DK0477290T3 (da) | 1997-07-21 |
WO1990015877A3 (en) | 1991-01-24 |
CA2062741A1 (en) | 1990-12-16 |
ATE148502T1 (de) | 1997-02-15 |
EP0753574A1 (en) | 1997-01-15 |
EP0477290A4 (en) | 1992-11-19 |
EP0477290B1 (en) | 1997-01-29 |
KR100196062B1 (ko) | 1999-06-15 |
ES2097149T3 (es) | 1997-04-01 |
DE69029856T2 (de) | 1997-09-04 |
DE69029856D1 (de) | 1997-03-13 |
EP0477290A1 (en) | 1992-04-01 |
JPH04506153A (ja) | 1992-10-29 |
WO1990015877A2 (en) | 1990-12-27 |
KR100201662B1 (en) | 1999-06-15 |
KR100201663B1 (en) | 1999-06-15 |
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