KR890701771A - Hiv 항원에 특이적인 단일 클론 항체 - Google Patents

Hiv 항원에 특이적인 단일 클론 항체

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Publication number
KR890701771A
KR890701771A KR1019890701259A KR890701259A KR890701771A KR 890701771 A KR890701771 A KR 890701771A KR 1019890701259 A KR1019890701259 A KR 1019890701259A KR 890701259 A KR890701259 A KR 890701259A KR 890701771 A KR890701771 A KR 890701771A
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South Korea
Prior art keywords
cell line
antigen
antigens
monoclonal antibody
nuclear
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KR1019890701259A
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English (en)
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KR960016304B1 (ko
Inventor
케니스 에취. 코트라이트
데이비드 이. 호프하인즈
카로울 설리반
개리 피. 토에드터
Original Assignee
마이론 씨.카스
쿠울터 코오포레이숀
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Publication of KR890701771A publication Critical patent/KR890701771A/ko
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Publication of KR960016304B1 publication Critical patent/KR960016304B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1054—Lentiviridae, e.g. HIV, FIV, SIV gag-pol, e.g. p17, p24
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • AIDS & HIV (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음

Description

HIV 항원에 특이적인 단일 클론 항체
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (12)

  1. 공통 에피토프를 지니는 HIV 핵 항원 군을 인지하며 HIV 외막 항원은 본질적으로 인지하지 못하는 KC-57 단일 클론 항체를 생산하는 하이브리도마기법에 의해 개발된 세포주.
  2. 제1항에 있어서, 핵 항원이 p55, p24 및 추가의 분해된 항원을 포함하는 세포주.
  3. 제1항에 있어서, 단일 클론 항체가 쥐의 비세포(splenocytes)로부터 유래되는 세포주.
  4. 제1항에 있어서, 비세포가 일련의 상이한 임뮤노겐 면역법으로부터 개발된 세포주.
  5. 제4항에 있어서, 임뮤노겐이 분리된 LAV로 감염된 세포주, 정제된 HIV 비루스 및 gp 160/120을 차례로 주입시킨 것으로 구성된 세포주.
  6. 제3항에 있어서, 비세포가 새앙쥐 골수종 세포와 융합된 세포주.
  7. 매릴랜드, 록빌에 소재하는 아메리칸 타입 컬쳐 콜렉숀에 A.T.C.C. 번호 HB 9585로 기탁된 하이브리드 세포주 시료.
  8. 매릴랜드, 록빌에 소재하는 아메리칸 타입 컬쳐 콜렉숀에 A.T.C.C. 번호 HB 9585로 기탁된 하이브리도마 시료로부터 생산된 단일 클론 항체.
  9. A. 다중 비루스성 분리물로부터 제조된 항원을 새앙쥐에 주사하고, 새앙쥐 비장세포를 수거하여 새앙쥐 골수종 세포주와 융합시키고 ; B. 융합된 세포를 선별하여 p55, p24 및 특정의 분해된 핵 항원을 포함하는 HIV 핵 항원군상의 KC-57 항원에 특이적으로 결합하는 단일 클론 항체를 분리하고 ; C. KC-57 항원의 면역 글로불린 이소타입(isotype)이 새앙쥐 IgG1인 것을 특징으로 하는 하이브리도마기법에 의해 생산되는 세포주로부터 유래하는 단일 클론 항체.
  10. HIV 핵 항원군의 KC-57 항원에 결합하여 HIV 외막 항원에는 본질적으로 결합하지 않는 단일 클론 항체.
  11. 제10항에 있어서, 핵 항원이 p55 및 p24 항원인 것을 특징으로 하는 단일 클론 항체.
  12. 제11항에 있어서, 핵 항원이 p39 및 p33 항원인 것을 특징으로 하는 단일 클론 항체.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890701259A 1987-11-06 1989-07-05 Hiv 항원에 특이적인 단일 클론 항체 KR960016304B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/118,145 US4888290A (en) 1987-11-06 1987-11-06 Monoclonal antibody specific to HIV antigens
US07/118,145 1987-11-06
PCT/US1988/001997 WO1989004376A1 (en) 1987-11-06 1988-06-10 Monoclonal antibody specific to hiv antigens

Publications (2)

Publication Number Publication Date
KR890701771A true KR890701771A (ko) 1989-12-21
KR960016304B1 KR960016304B1 (ko) 1996-12-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890701259A KR960016304B1 (ko) 1987-11-06 1989-07-05 Hiv 항원에 특이적인 단일 클론 항체

Country Status (13)

Country Link
US (1) US4888290A (ko)
EP (1) EP0415920B1 (ko)
JP (1) JP2710972B2 (ko)
KR (1) KR960016304B1 (ko)
CN (1) CN1025219C (ko)
AU (1) AU612686B2 (ko)
CA (1) CA1339350C (ko)
DE (1) DE3854502T2 (ko)
ES (1) ES2016427A6 (ko)
IE (1) IE61241B1 (ko)
IL (1) IL87535A (ko)
WO (1) WO1989004376A1 (ko)
ZA (1) ZA884651B (ko)

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US5104790A (en) * 1987-06-29 1992-04-14 Genetic Systems Corporation Monoclonal antibodies to specific antigenic regions of the human immunodeficiency virus and methods for use
US4886742A (en) * 1987-06-15 1989-12-12 Coulter Corporation Enzyme immunoassay for detecting HIV antigens in human sera
US5210181A (en) * 1989-05-15 1993-05-11 Akzo N.V. T-lymphotropic retrovirus peptide
ZA903492B (en) * 1989-05-15 1991-02-27 Akzo Nv T-lymphotropic retrovirus monoclonal antibodies
ATE126238T1 (de) * 1990-06-01 1995-08-15 Lepetit Spa Monoklonale antikörper gegen gag-protein- vorläufer-p55 von hiv-virus.
US5164293A (en) * 1990-09-25 1992-11-17 Coulter Corporation Monoclonal antibody for detecting HTLV-I, HTLV-II and STLV-I viruses
EP0519866A1 (en) * 1991-06-18 1992-12-23 Ciba-Geigy Ag Novel anti-HIV antibodies
US5451525A (en) * 1992-02-14 1995-09-19 Coulter Corporation Method and materials for determining particle count in a flow cytometer
US6319665B1 (en) 1994-06-07 2001-11-20 Inverness Medical Technology, Inc. Home test kit and method with telephone verification of results
AU5082698A (en) * 1996-10-23 1998-05-15 Oravax, Inc Heat inactivation of viruses in antibody preparations
US5891994A (en) * 1997-07-11 1999-04-06 Thymon L.L.C. Methods and compositions for impairing multiplication of HIV-1
US5902722A (en) * 1997-12-05 1999-05-11 The Perkin-Elmer Corporation Method of detecting organisms in a sample
US5915307A (en) * 1998-01-29 1999-06-29 Suncast Corporation Sports shelf
NO311807B1 (no) * 1999-03-04 2002-01-28 Bionor Immuno As HIV-peptider, antigener, vaksinepreparater, immunoassay- testsett og en metode for påvisning av antistoffer fremkalt av HIV
US6399067B1 (en) * 2000-04-28 2002-06-04 Thymon L.L.C. Methods and compositions for impairing multiplication of HIV-1
US6818392B2 (en) * 2000-12-06 2004-11-16 Abbott Laboratories Monoclonal antibodies to human immunodeficiency virus and uses thereof
CN101213212A (zh) 2005-02-15 2008-07-02 西蒙有限公司 削弱hiv-1增殖的方法和组合物
US20080108054A1 (en) * 2006-08-28 2008-05-08 Jayasri Basu Detecting early HIV infection in genital tract cells and secretions
CA2793959C (en) 2010-03-25 2019-06-04 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
CA2832109C (en) 2011-06-10 2021-07-06 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
AU2012216792A1 (en) 2011-09-12 2013-03-28 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing HIV-1 proteins by broadly neutralizing antibodies
EP2586461A1 (en) 2011-10-27 2013-05-01 Christopher L. Parks Viral particles derived from an enveloped virus
EP2679596B1 (en) 2012-06-27 2017-04-12 International Aids Vaccine Initiative HIV-1 env glycoprotein variant
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
EP2873423B1 (en) 2013-10-07 2017-05-31 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers
US10174292B2 (en) 2015-03-20 2019-01-08 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
US9931394B2 (en) 2015-03-23 2018-04-03 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
CN110669133A (zh) * 2019-10-18 2020-01-10 普健生物（武汉）科技有限公司 利用尾静脉注射进行基因免疫开发小鼠单克隆抗体的方法

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Also Published As

Publication number Publication date
CN1033071A (zh) 1989-05-24
JP2710972B2 (ja) 1998-02-10
AU612686B2 (en) 1991-07-18
AU2133788A (en) 1989-06-01
DE3854502T2 (de) 1996-04-18
ES2016427A6 (es) 1990-11-01
WO1989004376A1 (en) 1989-05-18
IL87535A (en) 1993-01-14
DE3854502D1 (de) 1995-10-26
ZA884651B (en) 1990-03-28
KR960016304B1 (ko) 1996-12-09
IL87535A0 (en) 1989-01-31
EP0415920A1 (en) 1991-03-13
CN1025219C (zh) 1994-06-29
JPH03500483A (ja) 1991-02-07
CA1339350C (en) 1997-08-26
IE881799L (en) 1989-05-06
EP0415920B1 (en) 1995-09-20
EP0415920A4 (en) 1993-03-17
US4888290A (en) 1989-12-19
IE61241B1 (en) 1994-10-19

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