KR890007755A - 베타-배열의 n-아세틸뉴라민산을 인지할 수 있는 단일 클론성 항체 - Google Patents
베타-배열의 n-아세틸뉴라민산을 인지할 수 있는 단일 클론성 항체 Download PDFInfo
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- KR890007755A KR890007755A KR1019880014661A KR880014661A KR890007755A KR 890007755 A KR890007755 A KR 890007755A KR 1019880014661 A KR1019880014661 A KR 1019880014661A KR 880014661 A KR880014661 A KR 880014661A KR 890007755 A KR890007755 A KR 890007755A
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- South Korea
- Prior art keywords
- balb
- beta
- acetylneuraminic acid
- mice
- derived
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- SQVRNKJHWKZAKO-PFQGKNLYSA-N N-acetyl-beta-neuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-PFQGKNLYSA-N 0.000 title claims description 8
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 title claims description 7
- 238000000034 method Methods 0.000 claims description 12
- 241000699670 Mus sp. Species 0.000 claims 6
- 210000004408 hybridoma Anatomy 0.000 claims 6
- 210000004027 cell Anatomy 0.000 claims 5
- 241001465754 Metazoa Species 0.000 claims 4
- 206010035226 Plasma cell myeloma Diseases 0.000 claims 4
- 201000000050 myeloid neoplasm Diseases 0.000 claims 4
- 241000699666 Mus <mouse, genus> Species 0.000 claims 3
- 210000003719 b-lymphocyte Anatomy 0.000 claims 3
- 230000007910 cell fusion Effects 0.000 claims 3
- 230000016784 immunoglobulin production Effects 0.000 claims 2
- 210000004988 splenocyte Anatomy 0.000 claims 2
- 101710098119 Chaperonin GroEL 2 Proteins 0.000 claims 1
- 241000282412 Homo Species 0.000 claims 1
- 108060003951 Immunoglobulin Proteins 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 241001222774 Salmonella enterica subsp. enterica serovar Minnesota Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000001154 acute effect Effects 0.000 claims 1
- 230000000890 antigenic effect Effects 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 201000011510 cancer Diseases 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000003053 immunization Effects 0.000 claims 1
- 102000018358 immunoglobulin Human genes 0.000 claims 1
- 208000027866 inflammatory disease Diseases 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 내지 3도는 효소-표지 항체 기법에 의해 얻어진, 본 발명의 단일클론성 항체와 베타-베열의 N-아세틸뉴라민산의 다양한 유도체간의 교차반응 결과를 예시하는 도표이다.
Claims (17)
- 에피토프 또는 항원 결정기로서의 베타-베열의 N-아세틸뉴라민산 또는 그의 유도체에 특이적인 단일클론성 항체를 생성하는 하이브리도마.
- 제1항에 있어서, ATCC HB 9619인 것을 특징으로 하는 하이브리도마.
- 에피도프로서의 베타-배열의 N-아세틸뉴라민 산 또는 그의 유도체에 특이적인 단일클론성 항체를 생성할 수 있는 하이브리도마의 생성방법에 있어서, (i) 항원으로서 베타-배열의 N-아세틸뉴라민 산 또는 그의 유도체로 동물을 면역함으로써 얻어진 B세포 또는 임파구를 (ii) 골수종 세포와 융합시키는 것을 포함하는 방법.
- 제3항에 있어서, 세포 융합에 의하여 생성된 하이브리도마가 추가로 베타-배열의 N-아세틸뉴라민 산 또는 그의 유도체에 특이적인 항체생산 여부에 대하여 스크린되는 것을 특징으로 하는 방법.
- 제3항에 있어서, 동물이 Balb/c 마우스인 것을 특징으로 하는 방법.
- 제5항에 있어서, B세포는 Balb/c 마우스로부터 유도된 비장세포인 것을 특징으로 하는 방법.
- 제3항에 있어서, 골수종 세포는 사람, 쥐 또는 마우스로부터 유도된는 것들인 것을 특징으로 하는 방법.
- 제7항에 있어서, 골수종 세포가 Balb/c 마우스로부터 유도되는 것을 특징으로 하는 방법.
- 제3항에 있어서, 동물은 Balb/c 마우스로부터 유도되고 B세포는 Balb/c마우스로부터 유도된 비장세포인 것을 특징으로 하는 방법.
- 제3항에 있어서, 동물은 Balb/c 마우스이며, 골수종세포는 Balb/c 마우스로부터 유도되고 N-아세틸뉴라민 산 유도체는 다음 식에 의하여 표시되는 화합물(A)인 것을 특징으로 하는 방법.
- 제10항에 있어서, 세포 융합에 의해 생성된 하이브리도마가 항-화합물(A) 항체 생성에 대해 스크린되는 것을 특징으로 하는 방법.
- 제3항에 있어서, 세포 융합이 융합하는 세포에 대한 제로서 폴리에틸렌 글리콜의 존재하에 수행되는 것을 특징으로 하는 방법.
- 제3항에 있어서, 면역감작은 보조액으로서 살모넬라 미네소타 R595를 사용하여 수행되는 것을 특징으로 하는 방법.
- 에피토프 또는 항원결정기로서의 베타- 배열의 N-아세틸뉴라민 산에 특이적인 단일클론성 항체.
- 제14항에 있어서, IgG형 면역글로불린 분자인 것을 특징으로하는 단일클론성 항체.
- 제14항에 있어서, 하이브리도마 ATCC HB 9619에 의해 생성되는 것을 특징으로 하는 단일클론성 항체.
- 제14항에 있어서, 혈액 또는 요중에 존재하는 유리 N-아세틸 뉴라민 산을 검출 및 정량함으로써 악성종양 또는 급성 염증성 질병의 진단에 응용되는 것을 특징으로 하는 단일 클론성 항체.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62-287119 | 1987-11-13 | ||
| JP28711987 | 1987-11-13 | ||
| JP63026848A JPH02486A (ja) | 1987-11-13 | 1988-02-08 | 遊離ｎ−アセチルノイラミン酸を認識するモノクローナル抗体 |
| JP63-26848 | 1988-02-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR890007755A true KR890007755A (ko) | 1989-07-05 |
| KR960004855B1 KR960004855B1 (ko) | 1996-04-16 |
Family
ID=26364692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019880014661A KR960004855B1 (ko) | 1987-11-13 | 1988-11-08 | 베타-배열의 n-아세틸뉴라민산을 인지할 수 있는 단일클론성 항체 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5158886A (ko) |
| EP (1) | EP0316818B1 (ko) |
| JP (1) | JPH02486A (ko) |
| KR (1) | KR960004855B1 (ko) |
| AU (1) | AU614553B2 (ko) |
| CA (1) | CA1336763C (ko) |
| DE (1) | DE3881344T2 (ko) |
| ES (1) | ES2054772T3 (ko) |
| FI (1) | FI90989C (ko) |
| HU (1) | HU204897B (ko) |
| IL (1) | IL88322A (ko) |
| NO (1) | NO174110C (ko) |
| NZ (1) | NZ226937A (ko) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03242553A (ja) * | 1990-02-19 | 1991-10-29 | Mect Corp | エピシアロ複合糖質を含む製剤 |
| WO2005010485A2 (en) * | 2003-07-15 | 2005-02-03 | The Regents Of The University Of California | Methods for detecting and analyzing n-glycolylneuraminic acid (neu5gc) in biological materials |
| CN100401753C (zh) * | 2004-07-20 | 2008-07-09 | 上海乐金广电电子有限公司 | 数码tv接收用智能天线控制系统 |
| WO2013151649A1 (en) | 2012-04-04 | 2013-10-10 | Sialix Inc | Glycan-interacting compounds |
| CA2967595A1 (en) | 2014-11-12 | 2016-05-19 | Siamab Therapeutics, Inc. | Glycan-interacting compounds and methods of use |
| US9879087B2 (en) | 2014-11-12 | 2018-01-30 | Siamab Therapeutics, Inc. | Glycan-interacting compounds and methods of use |
| SG11201803213XA (en) | 2015-11-12 | 2018-05-30 | Siamab Therapeutics Inc | Glycan-interacting compounds and methods of use |
| EP3541847A4 (en) | 2016-11-17 | 2020-07-08 | Seattle Genetics, Inc. | COMPOUNDS INTERACTING WITH GLYCANE AND METHODS OF USE |
| KR20240044544A (ko) | 2017-03-03 | 2024-04-04 | 씨젠 인크. | 글리칸-상호작용 화합물 및 사용 방법 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59164798A (ja) * | 1983-03-09 | 1984-09-17 | Rikagaku Kenkyusho | シアル酸含有糖脂質誘導体 |
-
1988
- 1988-02-08 JP JP63026848A patent/JPH02486A/ja active Pending
- 1988-11-08 IL IL8832288A patent/IL88322A/en not_active IP Right Cessation
- 1988-11-08 KR KR1019880014661A patent/KR960004855B1/ko not_active IP Right Cessation
- 1988-11-08 US US07/268,895 patent/US5158886A/en not_active Expired - Fee Related
- 1988-11-10 CA CA000582783A patent/CA1336763C/en not_active Expired - Fee Related
- 1988-11-10 FI FI885169A patent/FI90989C/fi not_active IP Right Cessation
- 1988-11-11 EP EP88118876A patent/EP0316818B1/en not_active Expired - Lifetime
- 1988-11-11 AU AU24982/88A patent/AU614553B2/en not_active Ceased
- 1988-11-11 DE DE8888118876T patent/DE3881344T2/de not_active Expired - Fee Related
- 1988-11-11 NO NO885053A patent/NO174110C/no unknown
- 1988-11-11 ES ES88118876T patent/ES2054772T3/es not_active Expired - Lifetime
- 1988-11-11 NZ NZ226937A patent/NZ226937A/en unknown
- 1988-11-11 HU HU885786A patent/HU204897B/hu not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HUT48676A (en) | 1989-06-28 |
| NO885053L (no) | 1989-05-16 |
| EP0316818A2 (en) | 1989-05-24 |
| AU2498288A (en) | 1989-05-18 |
| CA1336763C (en) | 1995-08-22 |
| AU614553B2 (en) | 1991-09-05 |
| ES2054772T3 (es) | 1994-08-16 |
| FI885169A (fi) | 1989-05-14 |
| DE3881344D1 (de) | 1993-07-01 |
| US5158886A (en) | 1992-10-27 |
| EP0316818A3 (en) | 1990-08-22 |
| HU204897B (en) | 1992-02-28 |
| FI90989B (fi) | 1994-01-14 |
| DE3881344T2 (de) | 1993-09-09 |
| IL88322A (en) | 1994-05-30 |
| FI885169A0 (fi) | 1988-11-10 |
| NO174110B (no) | 1993-12-06 |
| KR960004855B1 (ko) | 1996-04-16 |
| FI90989C (fi) | 1994-04-25 |
| JPH02486A (ja) | 1990-01-05 |
| NO174110C (no) | 1994-03-16 |
| EP0316818B1 (en) | 1993-05-26 |
| NZ226937A (en) | 1991-02-26 |
| NO885053D0 (no) | 1988-11-11 |
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