KR890701596A - 알카로이드 추출 - Google Patents
알카로이드 추출Info
- Publication number
- KR890701596A KR890701596A KR1019880701770A KR880701770A KR890701596A KR 890701596 A KR890701596 A KR 890701596A KR 1019880701770 A KR1019880701770 A KR 1019880701770A KR 880701770 A KR880701770 A KR 880701770A KR 890701596 A KR890701596 A KR 890701596A
- Authority
- KR
- South Korea
- Prior art keywords
- tissue
- aqueous medium
- extracting
- soluble component
- soluble
- Prior art date
Links
- 238000000605 extraction Methods 0.000 title claims 6
- 229930013930 alkaloid Natural products 0.000 title claims 4
- 150000003797 alkaloid derivatives Chemical class 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 16
- 239000012736 aqueous medium Substances 0.000 claims 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical group CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 5
- 239000003638 chemical reducing agent Substances 0.000 claims 4
- 239000000284 extract Substances 0.000 claims 4
- 239000012279 sodium borohydride Substances 0.000 claims 4
- 229910000033 sodium borohydride Inorganic materials 0.000 claims 4
- 230000002378 acidificating effect Effects 0.000 claims 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 2
- 241000220317 Rosa Species 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000012267 brine Substances 0.000 claims 2
- 239000003960 organic solvent Substances 0.000 claims 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- FFRFGVHNKJYNOV-DOVUUNBWSA-N 3',4'-Anhydrovinblastine Chemical compound C([C@@H](C[C@]1(C(=O)OC)C=2C(=CC3=C([C@]45[C@H]([C@@]([C@H](OC(C)=O)[C@]6(CC)C=CCN([C@H]56)CC4)(O)C(=O)OC)N3C)C=2)OC)C=C(C2)CC)N2CCC2=C1NC1=CC=CC=C21 FFRFGVHNKJYNOV-DOVUUNBWSA-N 0.000 claims 1
- 240000001829 Catharanthus roseus Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 239000011260 aqueous acid Substances 0.000 claims 1
- 239000006286 aqueous extract Substances 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 230000000593 degrading effect Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
- C07D519/04—Dimeric indole alkaloids, e.g. vincaleucoblastine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (18)
- 시. 로세우스 조직의 산성의 수성추출물의 가용성분으로 알카로이드를 추출하는 방법에 있어서, 상기 용해성 성분을 환원제로 처리하고, 처리된 용해성 성분을 유기용제로 추출하는 것을 특징으로 하는 알카로이드 추출방법.
- 제1항에서, 환원제는 수소화붕소나트륨인 것이 특징인 알카로이드 추출방법.
- 제2항에서, 유기용제는 초산에틸인 것이 특징인 알카로이드 추출방법.
- 카타란투스 로세우스 조직으로부터 3',4'-안하이드로빈볼라스틴을 추출하는 방법에 있어서,A) 산성의 pH를 갖는 수성매체로 조직을 추출하고, B) 생성된 추출물의 용해성성분을 비용해성 성문으로부터 분리하고, C) 용해성 성분을 환원제로 처리하고, 그리고 D) 처리된 용해성 성분을 유기용제로 추출하는 각 단계를 포함하는 것을 특징으로 하는 추출방법.
- 제4항에서, 환원제는 수소화 붕소나트륨인 것이 특징인 추출방법.
- 제5항에서, 처리된 용액중의 수소화 붕소 나트륨의 농도는 0.05-4.0mg/mL의 범위내인 것이 특징인 추출방법.
- 제4항에서, 용해성 성분 pH는 3,5-6,5 의 범위내인 것이 특징인 추출방법.
- 제4항에서, 수성매체는 물, 산성화수 및 염수중에서 선택되는 것이 특징인 추출방법.
- 제4항에서, 수성매체는 산성화수인것이 특징인 추출방법.
- 제9항에서, 수성매체는 염산에 의해 산성화된 물인 것이 특징인 추출방법.
- 제4항에서, 수성매체는 염수인 것이 특징인 추출방법.
- 제11항에서, 수성매체는 염화나트륨을 포함하며, 물농도는 0.9몰-3.0몰의 범위내인 것이 특징인 추출방법.
- 제4항에서, 산성의 pH를 갖는 수성매체로 조직을 추출한 후 과산화수소를 첨가하는 것이 특징인 추출방법.
- 제13항에서, 첨가되는 과산화 수소의 양은 8mM보다 큰 것이 특징인 추출방법.
- 제4항에서, 시, 로세우스 조직은 신선한 조직 또는 냉동된 신선한 조직인 것이 특징인 추출방법.
- 제15항에서, 시, 로세우스 조직은 추출하기에 앞서 셀루로오스 분해성 효소로 분해되는 것이 특징인 추출방법.
- 시,로세우스 조직으로부터 3',4'-안하이드로빈블라스틴을 추출하는 방법에 있어서, A) 신선한 시, 로세우스 조직을 액체질소중에서 분쇄하고, B) 상기 분쇄조직을 효소로 분해하여 구조직 식물조직과 화합되는 알카로이드를 유리하고, C) 상기 B) 단계에서 얻어진 조직을 수성의 산매체를 사용하여 추출하고, D) 상기 C)단계에서 얻어진 추출용액에 과산화수소를 첨가하고, E) 원심분리에 의해 용해성 성분과 비용해성 성분을 분리하고, F) 용해성 성분을 수소화붕소 나트륨으로 처리하고, 그리고 G) 처리된 용해성 성분을 초산에틸로 추출하는 각 단계를 포함하는 것이 특징인 추출방법.
- 제17항의 방법으로, 수시조제되는 3',4'-안하이드로빈블라스틴을 함유하는 추출물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US045,621 | 1987-05-01 | ||
| US07/045,621 US4831133A (en) | 1987-05-01 | 1987-05-01 | Extraction of alkaloids of Catharanthus roseus tissue |
| PCT/JP1988/000413 WO1988008425A1 (en) | 1987-05-01 | 1988-04-27 | Extraction of alkaloids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR890701596A true KR890701596A (ko) | 1989-12-21 |
| KR950013574B1 KR950013574B1 (ko) | 1995-11-09 |
Family
ID=21938973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019880701770A KR950013574B1 (ko) | 1987-05-01 | 1988-04-27 | 알카로이드 추출 방법 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4831133A (ko) |
| EP (1) | EP0312609B1 (ko) |
| JP (1) | JP2550405B2 (ko) |
| KR (1) | KR950013574B1 (ko) |
| CA (1) | CA1262443A (ko) |
| DE (1) | DE3881819T2 (ko) |
| FI (1) | FI91639C (ko) |
| WO (1) | WO1988008425A1 (ko) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5753507A (en) * | 1995-09-22 | 1998-05-19 | Novartis Finance Corporation | Plant geraniol/nerol 10-hydroxylase and DNA coding therefor |
| NL1009437C2 (nl) * | 1998-06-18 | 1999-12-21 | Xenobiosis | Extractiewerkwijze. |
| WO2000006582A1 (en) * | 1998-07-31 | 2000-02-10 | Goldsmith Seeds, Inc. | Trimeric and polymeric alkaloids |
| ES2312798T3 (es) * | 2002-02-28 | 2009-03-01 | Mallinckrodt Inc. | Metodo y sistema para la separacion y purificacion de al menos un alcaloide narcotico usando cromatografia preparativa de fase inversa. |
| CN103936769B (zh) * | 2014-04-30 | 2016-10-05 | 淮海工学院 | 一种制备高光学纯脱水长春碱的方法 |
| JP7282350B2 (ja) * | 2017-12-06 | 2023-05-29 | 国立大学法人高知大学 | タンパク質糖化反応阻害物質の製造方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU178084B (en) * | 1977-05-31 | 1982-03-28 | Richter Gedeon Vegyeszet | New process for the extraction of native vindoline,catharantine,3',4'-anhydrovinblastine,leurosine and,if desired,of other diinodle alkaloids from vinca rosea l.drogue |
| US4749787A (en) * | 1986-10-23 | 1988-06-07 | Harbor Branch Oceanographic Institution, Inc. | Process of isolating vinblastine from the plant catharanthis roseus |
-
1987
- 1987-05-01 US US07/045,621 patent/US4831133A/en not_active Expired - Lifetime
- 1987-08-20 CA CA000544980A patent/CA1262443A/en not_active Expired
-
1988
- 1988-04-27 WO PCT/JP1988/000413 patent/WO1988008425A1/en active IP Right Grant
- 1988-04-27 KR KR1019880701770A patent/KR950013574B1/ko not_active IP Right Cessation
- 1988-04-27 JP JP63503609A patent/JP2550405B2/ja not_active Expired - Lifetime
- 1988-04-27 EP EP88903929A patent/EP0312609B1/en not_active Expired - Lifetime
- 1988-04-27 DE DE88903929T patent/DE3881819T2/de not_active Expired - Fee Related
- 1988-12-30 FI FI886053A patent/FI91639C/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0312609A1 (en) | 1989-04-26 |
| KR950013574B1 (ko) | 1995-11-09 |
| FI91639C (fi) | 1994-07-25 |
| WO1988008425A1 (en) | 1988-11-03 |
| FI91639B (fi) | 1994-04-15 |
| FI886053A (fi) | 1988-12-30 |
| DE3881819D1 (de) | 1993-07-22 |
| EP0312609B1 (en) | 1993-06-16 |
| US4831133A (en) | 1989-05-16 |
| CA1262443A (en) | 1989-10-24 |
| JP2550405B2 (ja) | 1996-11-06 |
| JPH01503236A (ja) | 1989-11-02 |
| DE3881819T2 (de) | 1994-02-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| G160 | Decision to publish patent application | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| FPAY | Annual fee payment |
Payment date: 19981027 Year of fee payment: 4 |
|
| LAPS | Lapse due to unpaid annual fee |