KR890701480A - 질화세균에 의한 질화 및 질소제거 방법 - Google Patents

질화세균에 의한 질화 및 질소제거 방법

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Publication number
KR890701480A
KR890701480A KR1019890700488A KR890700488A KR890701480A KR 890701480 A KR890701480 A KR 890701480A KR 1019890700488 A KR1019890700488 A KR 1019890700488A KR 890700488 A KR890700488 A KR 890700488A KR 890701480 A KR890701480 A KR 890701480A
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South Korea
Prior art keywords
bacteria
nitriding
nitrate
nitrobacter
nitrite
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KR1019890700488A
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English (en)
Inventor
보크 에베르하르트
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원본미기재
테트라베르케 독토르 데어 나투르비센샤프텐 을리히 밴쉬 게엠베하
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Publication of KR890701480A publication Critical patent/KR890701480A/ko

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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/10Packings; Fillings; Grids
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/30Aerobic and anaerobic processes
    • C02F3/302Nitrification and denitrification treatment
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Hydrology & Water Resources (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

내용 없음

Description

질화세균에 의한 질화 및 질소제거 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도에서 제4도까지는 농도를 달리하는 아질산염의 존재하에서의 종속영양적(heterotrophic)생육 이후의 Nitrobacter nov spec T3속의 생육 곡선을 나타낸 도면임.

Claims (9)

  1. 초기 수용액중에 함유된 질산염을 질화세균에 의해 환원시키고, 이와같이 얻어진 반응생성물인 암모니아와 아질산염을 연속적인 산화공정에 의해 질산염으로 재전환시켜서, N2O나 NO와 같은 기체상의 질소 화합물을 방출시킴을 특징으로 하는 질화세균에 의한 질화 및 질소제거 방법.
  2. 제1항에 있어서, 질산염 및 아질산염의 환원을 각각 질화세균에 의해 낮은 산소분압에서 수행함을 특징으로 하는 방법.
  3. 제1항 또는 제2항에 있어서, 질화/질소제거 겸용방법을 암모니아, 아질산염 및/또는 질산염으로 오명된 천연수 또는 인공수에 대해 본래 위치의 (in situ)균을 공급함으로써 수행함을 특징으로 하는 방법.
  4. 제3항에 있어서, 상기 세균이 고정되고 이어서 활성탄, 제올라이트, 도자기류 또는 이온 교환기과 같은 구멍이 열린 다공성 지지체상에 건조된 질화세균인 것이 특징인 방법.
  5. 제4항에 있어서, 이용된 질화세균이 아질산염 산화세균, 예컨대,Nitrobacter속인것이 특징인 방법.
  6. 제4항에 있어서, 이요된 질화 세균이 아질산염 산화균과 암모니아 산화균인 것이 특징인 방법.
  7. 선택된 조건하에서 토양 시료로부터 분리되고 단독으로 20~30℃의 온도에서 교반이나 와동시키지 않으면서 종속영양적으로 생육하며 다음의 특징, 즉 (a) 혼합영양 및 무기독립영양적으로 보다는 종속영양적으로 더 잘 생육하고, b) 혼합영양적으로 배양시켰을 때 50㎎ 총 세포단백질/L의 높은 세포수율을 나타내며, c) 미호기적 조건하에서 최소 6개월이라는 장기간의 시간동안 배자에 단독으로 남겨졌을 때도 생존 능력을 과시하며, d) 산소포화 배지에서의 미호기적 종속영양적 생육 후 산소에 내성을 가지며, e) 산소를 공급한지 2-4일 후에 미호기적 생육후 질화 작용을 개시하고, f) 본 발명에 따른 방법에서 우수한 반응성을 나타내며, g ) 분자량이 80±3인 플라스미드를 갖는 것으로 특징지워지는 독일연방공화국 디-3400 괴팅겔에 소재하는 German Collection of Microorganisms는에 기탁번호 제DSM 4153호로 기탁된Nitrobacter속의 미생물.
  8. 생체내에서 공유적으로 밀집된 원형의 (Covalently colsed circular)DNA이고, 0.5% 아가로스 겔에서 전기영동적인 이동률로 측정했을 때 분자량이 약 80±3MD인 것이 특징인 제7항의Nitrobacter속 미생물로부터 유래한 플라스미드 DNA.
  9. 제8항에 있어서, 플라스미드 DNA가 제7항의Nitrobacter속의 미생물을 호기적 또는 미호기적 조건하에서 무기독립영양, 혼합영양 또는 종속영양적으로 세포배양시켜 생체내에서 복제시킴으로써 얻을 수 있는 것임을 특징으로 하는 플라스미드 DNA.
    ※ 참고사항:최초출원 내용에 의하여 공개하는 것임.
KR1019890700488A 1987-07-21 1988-07-19 질화세균에 의한 질화 및 질소제거 방법 KR890701480A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19873724027 DE3724027A1 (de) 1987-07-21 1987-07-21 Verfahren zur nitrifikation, einen hierfuer geeigneten mikroorganismus sowie die im mikroorganismus enthaltene plasmid-dna
DEP3724027.7 1987-07-21
PCT/EP1988/000651 WO1989000547A1 (en) 1987-07-21 1988-07-19 Process for nitrification and nitrogen removal by nitrifying bacteria

Publications (1)

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KR890701480A true KR890701480A (ko) 1989-12-20

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ID=6331993

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890700488A KR890701480A (ko) 1987-07-21 1988-07-19 질화세균에 의한 질화 및 질소제거 방법

Country Status (9)

Country Link
EP (1) EP0377595A1 (ko)
JP (1) JPH03501081A (ko)
KR (1) KR890701480A (ko)
AU (1) AU2131988A (ko)
DE (1) DE3724027A1 (ko)
DK (1) DK16090A (ko)
HU (1) HUT52742A (ko)
NO (1) NO900299L (ko)
WO (1) WO1989000547A1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101222602B1 (ko) * 2011-07-26 2013-01-16 부산대학교 산학협력단 혼합영양 탈질능력을 가지는 신규 카스텔라니엘라 균주와 이를 이용한 탈질공정

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3932521C1 (en) * 1989-09-29 1991-04-25 Nitra Gesellschaft Fuer Biotechnik Mbh, 2100 Hamburg, De Removing nitric oxide from enclosed atmos. - comprises culturing nitrifying bacteria in aq. suspension on membrane
DK112590D0 (da) * 1990-05-07 1990-05-07 S E Joergensen Fremgangsmaade til fjernelse af nitrogen fra vandige oploesninger
DE4137302A1 (de) * 1991-11-08 1992-04-30 Arbeitsstelle Tech Mikrobiolog Verfahren zur herstellung biologisch aktivierter koerper, diese koerper und ihre verwendung
NL1000794C2 (nl) * 1995-07-13 1997-01-14 Holding Company Belgie Nv Preparaat omvattende zeoliet, werkwijze voor de bereiding daarvan en toepassing daarvan voor het regelen van biologische omstandigheden in waters.
EP0805205A1 (en) * 1996-05-02 1997-11-05 Societe Des Produits Nestle S.A. Nitrate reduction system of Staphylococcus carnosus
US6881339B1 (en) 1997-10-30 2005-04-19 Sud-Chemie Ag Process for treating industrial and municipal waste water highly loaded with ammonium
DE19828175A1 (de) * 1997-10-30 1999-12-30 Sued Chemie Ag Verfahren zur Behandlung von mit Ammonium hochbelasteten Prozeßabwässern auf dem Abwassergebiet
GB9907210D0 (en) * 1999-03-30 1999-05-26 Stephenson Tom Nitrification monitor
CN111961659A (zh) * 2020-08-27 2020-11-20 电子科技大学中山学院 固定化材料、生物脱氮材料、制备方法、应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
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DE2811719A1 (de) * 1978-03-17 1979-09-27 Bayer Ag Reduktive behandlung chemischer stoffe, insbesondere abwasserinhaltstoffe, mit hilfe von mikroorganismen mit atmung oder daraus hergestellten praeparationen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101222602B1 (ko) * 2011-07-26 2013-01-16 부산대학교 산학협력단 혼합영양 탈질능력을 가지는 신규 카스텔라니엘라 균주와 이를 이용한 탈질공정

Also Published As

Publication number Publication date
EP0377595A1 (de) 1990-07-18
WO1989000547A1 (en) 1989-01-26
DE3724027C2 (ko) 1990-02-08
DK16090D0 (da) 1990-01-19
AU2131988A (en) 1989-02-13
HUT52742A (en) 1990-08-28
JPH03501081A (ja) 1991-03-14
DE3724027A1 (de) 1989-02-02
DK16090A (da) 1990-01-19
NO900299D0 (no) 1990-01-22
NO900299L (no) 1990-01-22

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