KR890016171A - N-말단 프롤린으로 출발하는 단백질의 제조방법 - Google Patents

N-말단 프롤린으로 출발하는 단백질의 제조방법 Download PDF

Info

Publication number
KR890016171A
KR890016171A KR1019890004645A KR890004645A KR890016171A KR 890016171 A KR890016171 A KR 890016171A KR 1019890004645 A KR1019890004645 A KR 1019890004645A KR 890004645 A KR890004645 A KR 890004645A KR 890016171 A KR890016171 A KR 890016171A
Authority
KR
South Korea
Prior art keywords
proline
terminal extension
extension sequence
starting
macrophage colony
Prior art date
Application number
KR1019890004645A
Other languages
English (en)
Inventor
하베르만 파울
Original Assignee
하이린히 벡커, 베른하르트베크
훽스트 아크티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 하이린히 벡커, 베른하르트베크, 훽스트 아크티엔게젤샤프트 filed Critical 하이린히 벡커, 베른하르트베크
Publication of KR890016171A publication Critical patent/KR890016171A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음

Description

N-말단 프롤린으로 출발하는 단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 pS203의 절단부위를 도시한 것이다. 제 2 도는 pS500의 절단부위를 도시한 것이다.

Claims (9)

  1. 유전자 조작에 의해 수득된, 짧은 N-말단 연장 서열을 갖는 전구체를 아미노펩티다제 P로 효소 절단 시킴을 특징으로하여, N-말단 프롤린으로 출발하는 단백질을 제조하는 방법.
  2. 제 1 항에 있어서, 짧은 N-말단 연장 서열이 메티오난 잔기를 함유하는 방법.
  3. 제 1 항에 있어서, 사용된 출발물질이, 일부만이 N 말단에 메티오닌 잔기를 갖는 단백질의 혼합물인 방법.
  4. 제 2 항에 있어서, 사용된 출발물질이, 일부만이 N 말단에 메티오닌 잔기를 갖는 단백질의 혼합물인 방법.
  5. 제 1 항에 있어서, 프롤린으로 출발하고 N-말단 연장 서열을 갖는 인간 과립구 대식세포 콜로니-자극인자 유도체를 사용한 방법.
  6. 제 2 항에 있어서, 프롤린으로 출발하고 N-말단 연장 서열을 갖는 인간과립구 대식세포 콜로니- 자극인자 유도체를 사용한 방법.
  7. 제 3 항에 있어서, 프롤린으로 출발하고 N-말단 연장 서열을 갖는 인간 과립구 대식세포 콜로니-자극인자 유도체를 사용한 방법.
  8. 제 4 항에 있어서, 플롤린으로 출발하고 N-말단 연장 서열을 갖는 인간 과립구 대식세포 콜로니-자극인자 유도체를 사용한 방법.
  9. 제 5 항에 있어서, 프롤린으로 출발하고 N-말단 연장 서열을 갖는 인간 과립구 대식세포 콜로니-자극인자 유도체를 사용한 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890004645A 1988-04-09 1989-04-08 N-말단 프롤린으로 출발하는 단백질의 제조방법 KR890016171A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3811921.8 1988-04-09
DE3811921A DE3811921A1 (de) 1988-04-09 1988-04-09 Verfahren zur herstellung von proteinen, die n-terminal mit prolin beginnen

Publications (1)

Publication Number Publication Date
KR890016171A true KR890016171A (ko) 1989-11-28

Family

ID=6351689

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890004645A KR890016171A (ko) 1988-04-09 1989-04-08 N-말단 프롤린으로 출발하는 단백질의 제조방법

Country Status (12)

Country Link
EP (1) EP0337264A3 (ko)
JP (1) JPH01304895A (ko)
KR (1) KR890016171A (ko)
AU (1) AU3253689A (ko)
DE (1) DE3811921A1 (ko)
DK (1) DK169889A (ko)
FI (1) FI891652A (ko)
HU (1) HUT51676A (ko)
IL (1) IL89877A0 (ko)
NO (1) NO891451L (ko)
PT (1) PT90225A (ko)
ZA (1) ZA892551B (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3822045A1 (de) * 1988-06-30 1990-01-11 Hoechst Ag Verfahren zur herstellung von proteinen
GB9017008D0 (en) * 1990-08-02 1990-09-19 Erba Carlo Spa Process for the enzymatic preparation of basic fibroblast growth factor
US7504372B2 (en) 2004-09-08 2009-03-17 Clariant Produkte (Deutschland) Gmbh Mixtures of bleaching agents
DE102004043360A1 (de) 2004-09-08 2006-03-09 Clariant Gmbh Bleichaktivator-Mischungen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
JPS60500043A (ja) * 1982-12-10 1985-01-17 ノルデイスク・インスリンラボラトリウム 原核細胞又は真核細胞中で合成される融合蛋白質から成熟蛋白質を作る方法
ATE251217T1 (de) * 1984-08-16 2003-10-15 Savient Pharmaceuticals Inc Methode zur herstellung von menschlichen wachstumshormonen
WO1986007380A1 (en) * 1985-06-04 1986-12-18 Takeda Chemical Industries, Ltd. Process for preparing polypeptide
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase
DE3822045A1 (de) * 1988-06-30 1990-01-11 Hoechst Ag Verfahren zur herstellung von proteinen

Also Published As

Publication number Publication date
JPH01304895A (ja) 1989-12-08
DE3811921A1 (de) 1989-10-19
FI891652A (fi) 1989-10-10
IL89877A0 (en) 1989-12-15
DK169889A (da) 1989-10-10
EP0337264A3 (de) 1990-08-01
DK169889D0 (da) 1989-04-07
NO891451L (no) 1989-10-10
AU3253689A (en) 1989-10-12
PT90225A (pt) 1989-11-10
EP0337264A2 (de) 1989-10-18
NO891451D0 (no) 1989-04-07
HUT51676A (en) 1990-05-28
ZA892551B (en) 1989-12-27
FI891652A0 (fi) 1989-04-06

Similar Documents

Publication Publication Date Title
DE69020794D1 (de) Enzymatische Entfernung einer aminoterminalen Proteinsequenz.
NO872988D0 (no) Pattedyr-interleukin-4.
ATE26871T1 (de) Verbindungsstange, insbesondere pleuelstange.
KR890007750A (ko) 인간 조직 인자 억제제의 dna 클론
ES548667A0 (es) Un procedimiento para la preparacion de polipeptidos.
KR890000517A (ko) 하이브리드 단백질 c 및 이의 제조방법
KR870000356A (ko) 메티오닐 단백질로부터 n-말단 메티오닌을 제거하는 방법
AU567747B2 (en) Early detection of mammalian pregnancy
KR890016171A (ko) N-말단 프롤린으로 출발하는 단백질의 제조방법
GB2165761A (en) Bipolar double-acting electrocoagulator
KR930701487A (ko) 식물성 독소 겔로닌의 단백질 구조물
AU4291285A (en) Compositions of polycycloalkenyl-terminated, unsaturated polyesters or polyesteramides and vinyl-reactive plasticizers therefor
KR910001052A (ko) 단백질 제조방법
KR900005897A (ko) 곤약-첨가 식품
KR890008166A (ko) 융합 단백질의 선택적 분해 방법
KR920018076A (ko) 신규 폴리펩티드
KR910001061A (ko) 재조합 아비폭스비루스
KR870004142A (ko) 인터로이킨-2 유도체의 제조방법
KR840002346A (ko) 펩타이드의 제조방법
KR910011898A (ko) 데스(64,65)-프로인슐린의 제조방법
DE58906820D1 (de) Verfahren zur Herstellung von Aralkylhydroperoxiden.
ES2051183A1 (es) Composicion explosiva
KR900014598A (ko) 변형된 pe_40의 제조
KR880006995A (ko) 밤가루를 이용한 면류의 제조방법
ATE110775T1 (de) Verfahren zur herstellung von hirudin.

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid