KR890014147A - 항체를 고정시킨 친화성 크로마토그래피를 위한 담체 및 이의 제조방법 - Google Patents

항체를 고정시킨 친화성 크로마토그래피를 위한 담체 및 이의 제조방법 Download PDF

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KR890014147A
KR890014147A KR1019890002875A KR890002875A KR890014147A KR 890014147 A KR890014147 A KR 890014147A KR 1019890002875 A KR1019890002875 A KR 1019890002875A KR 890002875 A KR890002875 A KR 890002875A KR 890014147 A KR890014147 A KR 890014147A
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carrier
antibody
polyethylene glycol
immobilized
modified
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KR910004069B1 (ko
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에이조 사다
시게오 가또오
아끼히꼬 콘도
마사아끼 기시무라
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노기 사다오
짓소 코포레이션
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/3092—Packing of a container, e.g. packing a cartridge or column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
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    • B01J2220/00—Aspects relating to sorbent materials
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    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58—Use in a single column

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Inorganic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음.

Description

항체를 고정시킨 친화성 크로마토그래피를 위한 담체 및 이의 제조방법
제 1 도는 본 발명의 친화성 크로마토그래피를 위한 담체들의 활성화가 몇가지 종류의 프로테아제의 반복처리로 낮아지는 관계를 도시한 것이다.
제 2 도는 불용성 담체상에 고정된 항체의 활성화 및 활성화된 PEG의 몰비의 변화가 프로나제의 반복처리로 낮아지는 관계를 도시한 것이다

Claims (10)

  1. 불용성 담체, 및 활성화된 폴리에틸렌글리콜에 의해 변형되어 불용성 담체상에 고정화된 항체를 함유함을 특징으로 하는 친화성 크로마토그래피를 위한 담체.
  2. 제 1 항에 있어서, 활성화된 폴리에틸렌글리콜이 메톡시 폴리에틸렌 글리콜과 시아노르산 클로라이드의 축합 물질인 담체.
  3. 제 1 항에 있어서, 변형된 항체를, 메톡시 폴리에틸렌글리콜과 시아누르산 클로라이드를 반응시켜 수득된 활성화된 폴리에틸렌글리콜에 항체를 가함으로써 수득하는 담체.
  4. 제 1 항에 있어서, 항체가 폴리클로날 항체 또는 모노클로날 항체인 담체.
  5. 제 1 항에 있어서, 담체상에 고정화된 항체를, 할로겐과 시안산, 에피클로로하이드린, 비스에폭사이드 및 하이드라지드 유도체의 방법들로 이루어진 그룹중에서 선택되는 방법에 의해 수득하는 담체.
  6. 제 6 항에 있어서, 셀룰로즈, 덱스트란, 아가로즈,폴리아크릴아미드 및 다공성 유리로 이루어진 그룹중에서 선택되는 담체.
  7. 활성화된 폴리에틸렌글리콜(PEG)-변형된 항체를 고정시킨 담체를 제조하는 방법에 있어서, 활성화된 폴리에틸렌글리콜-변형된 항체를 제조하고, 수득된 항체를 담체상에 고정화시킴을 특징으로하는, 담체의 제조방법.
  8. 제 7 항에 있어서, 항체가 미리 항원으로 차단된 제조방법.
  9. 활성화된 폴리에틸렌글리콜(PEG)-변형된 항체를 고정화시킨 담체를 제조하는 방법에 있어서, 항체를 담체상에 고정시키고 고정화된 항체를 활성화된 PEG로 변형시킴을 특징으로 하는, 담체의 제조방법.
  10. 제 9항에 있어서, 항체가 미리 항원으로 차단된 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890002875A 1988-03-10 1989-03-09 항체를 고정시킨 친화성 크로마토그래피를 위한 담체 및 이의 제조방법 KR910004069B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP63-57299 1988-03-10
JP63057299A JP2761881B2 (ja) 1988-03-10 1988-03-10 抗体を固定化したアフイニテイクロマトグラフイ用担体

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KR890014147A true KR890014147A (ko) 1989-10-23
KR910004069B1 KR910004069B1 (ko) 1991-06-22

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US (1) US5147537A (ko)
EP (1) EP0332323B1 (ko)
JP (1) JP2761881B2 (ko)
KR (1) KR910004069B1 (ko)
CA (1) CA1337402C (ko)
DE (1) DE68900194D1 (ko)

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Also Published As

Publication number Publication date
KR910004069B1 (ko) 1991-06-22
EP0332323A1 (en) 1989-09-13
CA1337402C (en) 1995-10-24
JPH01229965A (ja) 1989-09-13
DE68900194D1 (de) 1991-09-19
JP2761881B2 (ja) 1998-06-04
EP0332323B1 (en) 1991-08-14
US5147537A (en) 1992-09-15

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