KR890006816A - 배양 동물 세포 내에서 단백질 생산을 증가시키기 위한 유전자 증폭 방법 - Google Patents

배양 동물 세포 내에서 단백질 생산을 증가시키기 위한 유전자 증폭 방법 Download PDF

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Publication number
KR890006816A
KR890006816A KR1019880013734A KR880013734A KR890006816A KR 890006816 A KR890006816 A KR 890006816A KR 1019880013734 A KR1019880013734 A KR 1019880013734A KR 880013734 A KR880013734 A KR 880013734A KR 890006816 A KR890006816 A KR 890006816A
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South Korea
Prior art keywords
gene
cells
animal cells
protein production
gene amplification
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KR1019880013734A
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English (en)
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유꼬 미주시마
히로미쯔 나까우찌
히로시 와타나베
꼬 오꾸무라
이사무 수가와라
Original Assignee
아다찌 카주오
훽스트 저팬 리미티드
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Publication of KR890006816A publication Critical patent/KR890006816A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70517—CD8
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

배양 동물 세포 내에서 단백질 생산을 증가시키기 위한 유전자 증폭 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pHE-LY2G의 유전자 지도를 도시한 것이다.

Claims (10)

  1. 동물 세포를 한 유전자 및 하이그로마이신 B-내성 유전자로 형질 감염시키고 세포를 하이그로마이신 B가 함유된 배지 중에서 배양시킴을 특징으로 하여, 동물 세포 내에서 한 유전자를 증폭시키는 방법.
  2. 제1항에 있어서, 언급된 세포가 포유동물 세포인 방법.
  3. 제1항에 있어서, 증폭시킬 유전자 및 내성 유전자를 동시에 도입시키는 방법.
  4. 제2항에 있어서, 증폭시킬 유전자 및 내성 유전자를 동시에 도입시키는 방법.
  5. 제1항에 따른 방법에 의해 수득된, 증가된 복제수의 유전자를 갖는 동물세포.
  6. 제2항에 따른 방법에 의해 수득된, 증가된 복제수의 유전자를 갖는 동물세포.
  7. 제3항에 따른 방법에 의해 수득된, 증가된 복제수의 유전자를 갖는 동물세포.
  8. 제5항에 청구된 세포를 배양시킴을 특징으로 하는 단백질 생산방법.
  9. 제6항에 청구된 세포를 배양시킴을 특징으로 하는 단백질 생산방법.
  10. 제7항에 청구된 세포를 배양시킴을 특징으로 하는 단백질 생산방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880013734A 1987-10-23 1988-10-21 배양 동물 세포 내에서 단백질 생산을 증가시키기 위한 유전자 증폭 방법 KR890006816A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62266529A JPH01108995A (ja) 1987-10-23 1987-10-23 培養動物細胞による蛋白質の産生を向上させるための遺伝子増幅方法
JP?62-266529 1987-10-23

Publications (1)

Publication Number Publication Date
KR890006816A true KR890006816A (ko) 1989-06-16

Family

ID=17432148

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880013734A KR890006816A (ko) 1987-10-23 1988-10-21 배양 동물 세포 내에서 단백질 생산을 증가시키기 위한 유전자 증폭 방법

Country Status (7)

Country Link
EP (1) EP0313009A3 (ko)
JP (1) JPH01108995A (ko)
KR (1) KR890006816A (ko)
AU (1) AU2408988A (ko)
DK (1) DK588188A (ko)
FI (1) FI884845A (ko)
PT (1) PT88807A (ko)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
CA1341345C (en) * 1987-05-08 2002-03-05 Hanne Ranch Johansen Expression of foreign genes in drosophila cells
IL86354A0 (en) * 1987-05-22 1988-11-15 Orion Yhtymae Oy Episomal vector for the expression of selected dna fragments coding for optional polypeptides in mammalian cells and methods for the production thereof
EP0319206A3 (en) * 1987-11-30 1990-04-18 Berlex Laboratories, Inc. Gene amplification

Also Published As

Publication number Publication date
AU2408988A (en) 1989-04-27
FI884845A (fi) 1989-04-24
EP0313009A2 (en) 1989-04-26
DK588188D0 (da) 1988-10-21
EP0313009A3 (en) 1989-11-23
JPH01108995A (ja) 1989-04-26
PT88807A (pt) 1989-07-31
DK588188A (da) 1989-04-24
FI884845A0 (fi) 1988-10-20

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