KR840002454A - 심부배양을 위한 세포 클로운의 자동 선별 방법 - Google Patents
심부배양을 위한 세포 클로운의 자동 선별 방법 Download PDFInfo
- Publication number
- KR840002454A KR840002454A KR1019820004974A KR820004974A KR840002454A KR 840002454 A KR840002454 A KR 840002454A KR 1019820004974 A KR1019820004974 A KR 1019820004974A KR 820004974 A KR820004974 A KR 820004974A KR 840002454 A KR840002454 A KR 840002454A
- Authority
- KR
- South Korea
- Prior art keywords
- micro
- titer plate
- cell
- inoculated
- well
- Prior art date
Links
- 238000012216 screening Methods 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 7
- 210000004027 cell Anatomy 0.000 claims 4
- 235000015097 nutrients Nutrition 0.000 claims 4
- 239000002207 metabolite Substances 0.000 claims 2
- 238000002965 ELISA Methods 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 238000004113 cell culture Methods 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000000706 filtrate Substances 0.000 claims 1
- 210000004602 germ cell Anatomy 0.000 claims 1
- 239000003547 immunosorbent Substances 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (6)
- 미량-역가 판의 각웰을 한개의 세포로 접종한, 여러개의 미량-역가판의 웰을 배양용기로서 자동방법으로 사용함을 특징으로 하여, 세포클로운을 심부 영양배지에 접종하고 배양 및 측정하는, 심부배양을 위한 세포 클로운의 선별 방법.
- 제1항에 있어서, a) 멸균한 미량-역가판에 멸균조건하에 영양용액을 자동적으로 도입하고, b) 미량-역가판의 각웰을 한개의 세포로 접종하며, c) 접종된 미량-역가판을 적층형태로 하여 심부 배양조건하에 배양시키고, d) 영양용액의 여액 또는 세포 배양 추출물을 제조하고 목적하는 대사 산물의 함량을 측정하는 방법.
- 제1항 또는 제2항에 있어서, 미량-역가판에 분배기의 저장용기내에서 이의 튜브 및 분배구와 함께 멸균한 영양용액을 도입시키는 방법.
- 제1항 내지 제3항중 하나 이상의 항에 있어서, 미량-역가판의 웰이 접종기구를 사용하여 유기체의 발아 세포 한개를 접종시킴을 특징으로 하는 방법.
- 제1항 내지 제4항중 하나 이상의 항에 있어서, 접종된 미량-역가판을 진탕 배양기 내에서 1 내지 5일간 배양시키는 방법.
- 제1항 내지 제5항중 하나 이상의 항에 있어서, 유기체에 의해 형성된 대사 산물을 효소 면역흡수 시험(=ELISA)으로 검출하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3144081.9 | 1981-11-06 | ||
DE3144081A DE3144081C2 (de) | 1981-11-06 | 1981-11-06 | Automatisiertes Verfahren zur Selektion von Zellklonen für Submerskulturen |
Publications (1)
Publication Number | Publication Date |
---|---|
KR840002454A true KR840002454A (ko) | 1984-07-02 |
Family
ID=6145757
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019820004974A KR840002454A (ko) | 1981-11-06 | 1982-11-04 | 심부배양을 위한 세포 클로운의 자동 선별 방법 |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0079528A1 (ko) |
JP (1) | JPS5881799A (ko) |
KR (1) | KR840002454A (ko) |
DE (1) | DE3144081C2 (ko) |
DK (1) | DK494382A (ko) |
ES (1) | ES8307898A1 (ko) |
IL (1) | IL67186A0 (ko) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19520420C2 (de) * | 1995-06-02 | 2002-12-05 | Symbio Herborn Group Gmbh & Co | Beimpfung von Nährböden mit Keimen |
JP2002509255A (ja) * | 1998-01-16 | 2002-03-26 | ディスカヴァリー テクノロジーズ アーゲー | 多数の類似する生物学的/化学的試験を微量分析規模で実施するための方法と装置 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5212982A (en) * | 1975-07-22 | 1977-01-31 | Olympus Optical Co Ltd | Apparatus for automatic incubation |
JPS5564795A (en) * | 1978-11-10 | 1980-05-15 | Olympus Optical Co Ltd | Method and apparatus for culturing living tissue or cell |
EP0015473A1 (de) * | 1979-02-28 | 1980-09-17 | F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft | Verfahren zum Immobilisieren von Zellen |
DE8129742U1 (de) * | 1981-10-12 | 1982-04-22 | Hoechst Ag, 6000 Frankfurt | "stapelbehaelter fuer mikrotiterplatten" |
-
1981
- 1981-11-06 DE DE3144081A patent/DE3144081C2/de not_active Expired
-
1982
- 1982-11-04 EP EP82110151A patent/EP0079528A1/de not_active Withdrawn
- 1982-11-04 KR KR1019820004974A patent/KR840002454A/ko unknown
- 1982-11-04 ES ES517112A patent/ES8307898A1/es not_active Expired
- 1982-11-05 IL IL67186A patent/IL67186A0/xx unknown
- 1982-11-05 JP JP57193562A patent/JPS5881799A/ja active Pending
- 1982-11-05 DK DK494382A patent/DK494382A/da not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
DE3144081A1 (de) | 1983-05-19 |
IL67186A0 (en) | 1983-03-31 |
ES517112A0 (es) | 1983-08-01 |
JPS5881799A (ja) | 1983-05-17 |
ES8307898A1 (es) | 1983-08-01 |
EP0079528A1 (de) | 1983-05-25 |
DE3144081C2 (de) | 1983-09-22 |
DK494382A (da) | 1983-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR9910580B1 (pt) | fermentador de estado sólido e procedimento para fermentação de estado sólido. | |
GB1466828A (en) | Diagnostic test system | |
SE7712375L (sv) | Cellodlingsforfarande | |
MX168694B (es) | Metodo para separar agentes antiespumantes durante el procesamiento de fermentaciones microbianas | |
Trainor | Indicator algal assays: laboratory and field approaches | |
KR840002454A (ko) | 심부배양을 위한 세포 클로운의 자동 선별 방법 | |
KR830007830A (ko) | 엔듀라시딘 및 그를 위한 미생물의 제조방법 | |
ES2044943T3 (es) | Uso de preparaciones de celulasa en el cultivo y en el uso de microorganismos que producen celulosa. | |
KR840000643A (ko) | 구아노신의 제조법 | |
JPS5599190A (en) | Preparation of heat-resistant hexokinase | |
DK148362C (da) | Fremgangsmaade til fremstilling af kolesterinesterase | |
ATE41953T1 (de) | Erhoehte hemmung des wachstums von hefezellen. | |
ATE86659T1 (de) | Kultureinrichtung. | |
SU687119A1 (ru) | Питательна среда дл выращивани клубеньковых бактерий | |
SU922139A1 (ru) | Питательна среда дл выращивани энтомопатогенных бактерий васILLUS popILLIae | |
WILT et al. | Studies on the interactions of aquatic bacteria and aquatic nematodes | |
JPS57132880A (en) | Preparation of choline oxidase by fermentation method | |
KR960017841A (ko) | 당전이 효소를 생성하는 미생물 선별용 배양접시의 제조방법 | |
KR830001367A (ko) | 열에 안정한 글루코아밀라제의 제법 | |
SU1100309A1 (ru) | Штамм @ @ - @ @ -2464 @ -продуцент рибонуклеаз | |
SU587155A1 (ru) | Штамм -19продуцент -ацетилглюкозаминидазы | |
SU803467A1 (ru) | Штамм @ -вибриона N14947 (серотип 57) дл серологической дифференциации @ -вибрионов | |
Tsuchiya et al. | Cultural Conditions for a Quantitative liquid Method Using the Spore Rec-assay System | |
ES2013827A6 (es) | Procedimiento de preparacion de ethisolide. | |
Cristino et al. | Mannose inhibition as a significant marker for differentiating among novobiocin-resistant staphylococci of relevance in clinical microbiology |