KR880011323A - 아세트산을 지표로한 배양방법 및 그 장치 - Google Patents

아세트산을 지표로한 배양방법 및 그 장치 Download PDF

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KR880011323A
KR880011323A KR1019880001409A KR880001409A KR880011323A KR 880011323 A KR880011323 A KR 880011323A KR 1019880001409 A KR1019880001409 A KR 1019880001409A KR 880001409 A KR880001409 A KR 880001409A KR 880011323 A KR880011323 A KR 880011323A
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노리오 시미즈
신이찌 후꾸조노
교오시 후지모리
노부꼬 니시무라
요오지 오다와라
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미다 가쓰시게
가부시기 가이샤 히다찌세이사꾸쇼
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    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C—CHEMISTRY; METALLURGY
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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    • Y10S435/8215—Microorganisms
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Abstract

내용없음

Description

아세트산을 지표로한 배양방법 및 그 장치
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 배양장치의 일실시예의 개요도이고,
제2도는 유전자 재조압 대장균을 사용하여 유가(流加) 배양을 행한 결과를 나타낸 그래프이고,
제3도는 배양액중의 아세트산 농도와 비증가 속도와의 관계를 일예를 나타낸 그래프이다.

Claims (20)

  1. 대사물을 생산하기 위하여 미생물 또는 동식물 세포를 배양하는 방법에 있어서, 배양액중의 아세트산 농도를 검지하고, 이 미생물 또는 동식물 세포에 의한 배양액중의 아세트산의 동화를 조절하는 것에 의해 아세트산 농도를 설정치 이하로 조절하는 공정을 포함하는 것을 특징으로 하는 배양방법.
  2. 제1항에 있어서, 이 아세트산 농도의 조절을 기질의 유가 조절에 의해 상기 아세트산의 동화를 조절하는 것에 의해 수행하는 배양방법.
  3. 제1항에 있어서, 아세트산 농도의 설정치가 15g/l 이하인 배양방법.
  4. 제1항 내지 3항중 어느 한 항에 있어서, 아세트산 농도가 설정치보다 높아진 경우는 기질유가량을 감소하건, 기질유가를 중지하는 것에 의해 미생물 또는 동식물세포에 아세트산을 동화시켜서 아세트산 농도를 저하시키는 배양방법.
  5. 제1항 내지 3항중 어느 한 항에 있어서, 아세트산 농도가 설정치보다 낮은 경우는 기질 유가량을 증가시키거나 기질유가를 재개하는 배양방법.
  6. 제1항에 있어서, 미생물 또는 동식물 세포가 아세트산을 동화하는 능력이 있는 미생물 또는 동식물 세포인 배양방법.
  7. 제1항에 있어서, 미생물 또는 동식물 세포가 유전자 재조합 미생물 또는 유전자 재조합 동식물 세포인 배양방법.
  8. 제2항에 있어서, 기질이 영양물질인 배양방법.
  9. 제9항에 있어서, 영양물질이 카스아미노산, 아미노산, 글루코스 및 효모엑기스로 이루어진 군에서 선택된 적어도 일종인 배양방법.
  10. 제1항에 있어서, 대사물이 효소 또는 생리활성물질인 배양방법.
  11. 제1항에 있어서, 대사물이 생산시키기 위하여 유도체를 첨가하는 배양방법.
  12. 제11항에 있어서, 유도체가 유전자 재조함 미생물 또는 유전자 재조합 동식물 세포가 보유되는 발현백타중의 프로모터에 작용하는 것인 배양방법.
  13. 제1항에 있어서, 미생물이 유전자 재조합 대장균인 배양방법.
  14. 제13항에 있어서, 유전자 재조합 미생물을 보유하는 발현 백타가 트립토판 프로모터, lac 프로코너, tac 프로모터 또는 PL 프로모터를 함유하는 것이 배양방법.
  15. 제13항 또는 제14항에 있어서, 유도제로서 프로모터가 trp 프로모터의 경우에는 3-β-인돌아크릴산을, lac 프로모터 또는 tac 프로모터의 경우는 이소프로필 -β-D티오갈락토시드를 사용하는 배앙방법.
  16. 제13항 또는 제14항에 있어서, 프로모터를 함유하는 유전자 재조합 대장균의 경우에는 트립토판을 함유하지 않거나 또는 트립토판 농도가 낮은 배지를 사용하고 PL 프로모터를 함유하는 유전자 재조합 대장균의 경우에는 배양액의 온도를 상승시켜 대사물 생산을 유도하는 배양방법.
  17. 대사물을 생산하기 위해서 미생물 또는 동식물 세포를 배양하기 위한 배양장치에 있어서, 배양을 행하는 배양조, 배양액중의 아세트산 농도 분석장치, 기질을 저장하기 위한 기질조, 기질을 유가하기 위한 유량 가변식의 기질 유가장치 및 분석장치로부터의 신호에 의거하여 기질 유가장치로부터의 기질 유량을 조절하는 신호를 출력하는 제어장치를 포함하는 것을 특징으로 하는 배양장치.
  18. 제17항에 있어서, 아세트산 농도 분석장치가 세관식 등속 전기 영동장치, 가스 크로마토그래프장치, 액체 크로마토그래프장치 또는 질량 분석계인 배양장치.
  19. 제17항에 있어서, 기질유가 장치가 기질유가용 펌프인 배양장치.
  20. 제17항에 있어서, 제어장치가 제어용 전자계산기인 배양장치.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880001409A 1987-03-23 1988-02-13 아세트산을 지표로한 배양방법 및 그 장치 KR900007630B1 (ko)

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JP62065710A JP2676511B2 (ja) 1987-03-23 1987-03-23 酢酸を指標とした培養方法及びその装置
JP65710 1987-03-23
JP62-65710 1987-03-23

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KR900007630B1 KR900007630B1 (ko) 1990-10-17

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US (1) US5139938A (ko)
EP (1) EP0283726B1 (ko)
JP (1) JP2676511B2 (ko)
KR (1) KR900007630B1 (ko)
CN (1) CN1040459C (ko)
DE (1) DE3871573T2 (ko)

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JP2686108B2 (ja) * 1988-10-07 1997-12-08 株式会社日立製作所 微生物の流加培養方法及び装置
KR0132666B1 (en) * 1989-03-14 1998-04-14 Hitachi Kk Method for controlling cultivation conditions for animal cells
DK0889949T3 (da) * 1996-03-13 2003-09-22 Delta Biotechnology Ltd Fermenteringskontrol
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
JP4463198B2 (ja) * 2003-03-05 2010-05-12 財団法人化学及血清療法研究所 大腸菌における異種蛋白質の製造方法
WO2005087940A1 (en) * 2004-03-11 2005-09-22 Wisconsin Alumni Research Foundation Genetically altered microorganisms with modified metabolism
US7842479B2 (en) * 2004-05-21 2010-11-30 The Board Of Regents Of The University Of Nebraska Methods for altering acetic acid production and enhancing cell death in bacteria
KR100813028B1 (ko) * 2007-01-02 2008-03-14 (주)상록이티씨 하ㆍ폐수 중의 질소성분 제거와 동시에 악취 저감을 위한 미생물 배양 공급기
JP4883067B2 (ja) 2008-09-29 2012-02-22 株式会社日立プラントテクノロジー 培養装置及び培養方法

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JPS5329985A (en) * 1976-08-27 1978-03-20 Kanegafuchi Chem Ind Co Ltd High concentration culturing of yeast
JPS5736983A (ja) * 1980-08-15 1982-02-27 Oriental Yeast Co Ltd Toryukabaiyonyorupankobonoseizoho
JPS5878584A (ja) * 1981-11-04 1983-05-12 Hitachi Ltd 培養基質の流加制御方法
JPS60224484A (ja) * 1984-04-23 1985-11-08 Nakano Vinegar Co Ltd 食酢の製造法およびその装置
DE3585176D1 (de) * 1984-06-22 1992-02-27 Hitachi Ltd Verfahren zum kontrollieren der zuechtung von rekombinanten.
JPH078231B2 (ja) * 1985-03-25 1995-02-01 株式会社日立製作所 培養制御方法及び培養制御装置
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置

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EP0283726A1 (en) 1988-09-28
CN1040459C (zh) 1998-10-28
JPS63233780A (ja) 1988-09-29
JP2676511B2 (ja) 1997-11-17
CN1036034A (zh) 1989-10-04
US5139938A (en) 1992-08-18
DE3871573T2 (de) 1993-01-21
KR900007630B1 (ko) 1990-10-17
EP0283726B1 (en) 1992-06-03
DE3871573D1 (de) 1992-07-09

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