KR880009123A - 활성화된 킬러세포의 개선된 제조방법 - Google Patents
활성화된 킬러세포의 개선된 제조방법 Download PDFInfo
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- KR880009123A KR880009123A KR1019880000685A KR880000685A KR880009123A KR 880009123 A KR880009123 A KR 880009123A KR 1019880000685 A KR1019880000685 A KR 1019880000685A KR 880000685 A KR880000685 A KR 880000685A KR 880009123 A KR880009123 A KR 880009123A
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- peripheral blood
- cells
- blood lymphocytes
- mononuclear cells
- lymphocytes
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- 238000004519 manufacturing process Methods 0.000 title claims 2
- 238000000034 method Methods 0.000 claims 19
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 claims 13
- 210000004027 cell Anatomy 0.000 claims 8
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims 8
- 150000008575 L-amino acids Chemical class 0.000 claims 6
- 229920001577 copolymer Polymers 0.000 claims 6
- KWKAKUADMBZCLK-UHFFFAOYSA-N 1-octene Chemical compound CCCCCCC=C KWKAKUADMBZCLK-UHFFFAOYSA-N 0.000 claims 4
- 125000005907 alkyl ester group Chemical group 0.000 claims 4
- 125000004432 carbon atom Chemical group C* 0.000 claims 4
- 239000000463 material Substances 0.000 claims 4
- 239000004711 α-olefin Substances 0.000 claims 4
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 3
- 239000005977 Ethylene Substances 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 3
- VXNZUUAINFGPBY-UHFFFAOYSA-N 1-Butene Chemical compound CCC=C VXNZUUAINFGPBY-UHFFFAOYSA-N 0.000 claims 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 2
- 102000000588 Interleukin-2 Human genes 0.000 claims 2
- 108010002350 Interleukin-2 Proteins 0.000 claims 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 2
- 206010028980 Neoplasm Diseases 0.000 claims 2
- 239000000806 elastomer Substances 0.000 claims 2
- 229920001971 elastomer Polymers 0.000 claims 2
- 235000013922 glutamic acid Nutrition 0.000 claims 2
- 239000004220 glutamic acid Substances 0.000 claims 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 150000003840 hydrochlorides Chemical class 0.000 claims 2
- 229920000554 ionomer Polymers 0.000 claims 2
- 210000004698 lymphocyte Anatomy 0.000 claims 2
- VSDUZFOSJDMAFZ-VIFPVBQESA-N methyl L-phenylalaninate Chemical group COC(=O)[C@@H](N)CC1=CC=CC=C1 VSDUZFOSJDMAFZ-VIFPVBQESA-N 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- TVMXDCGIABBOFY-UHFFFAOYSA-N n-Octanol Natural products CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 claims 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 2
- 102000008100 Human Serum Albumin Human genes 0.000 claims 1
- 108091006905 Human Serum Albumin Proteins 0.000 claims 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims 1
- 239000012980 RPMI-1640 medium Substances 0.000 claims 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 1
- 230000003013 cytotoxicity Effects 0.000 claims 1
- 231100000135 cytotoxicity Toxicity 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 229920000092 linear low density polyethylene Polymers 0.000 claims 1
- 239000004707 linear low-density polyethylene Substances 0.000 claims 1
- 210000000822 natural killer cell Anatomy 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 230000035699 permeability Effects 0.000 claims 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
- 229920000728 polyester Polymers 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 210000004881 tumor cell Anatomy 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
- C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
- A61K39/4643—Vertebrate antigens
- A61K39/4644—Cancer antigens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- General Engineering & Computer Science (AREA)
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- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Oncology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Mushroom Cultivation (AREA)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (20)
- 종양조직으로부터의 임파구 또는 말초혈 단핵세포를 배양하여 천연 킬러(Killer)세포에 저항성 있는 종양세포에 대해 세포독성을 갖는 세포집단을 제조하는 방법에 있어서, 말초혈 단핵세포, 그로부터 유래된 말초혈 임파구 및 종양조직으로부터 수득한 임파구로 구성된 그룹으로부터 선택된 세포집단을, 산소투과도가 적어도 약 1.8×105μm3(STP)/(m2.sec.Pa)이고 : 두께가 약 0.04mm 내지 약 0.23mm이고; (a) 에틸렌 및 탄소원자수 4 내지 10인 α-올레핀의, 밀도가 약 0.915 내지 0.925g/cm3인 공중합체, (b) 에틸렌 및 메타크릴산의 공중합체, (c) 아이오노머, 및 (d) 아이오노머/플리에스테르 탄성중합체 및 선형 저밀도의 폴리에틸렌 탄성중합체의 적층물 또는 공압출물로 구성된 그룹으로부터 선택된 물질인 공중합체성 필름물질로 제조한 밀폐된 용기내에서, 적어도 약 1.0×106세포/ml의 세포농도에서 배양시킴을 특징으로 하는 방법.
- 제1항에 있어서, 배양시키는 세포집단이 말초혈 단핵세포 및 그로부터 유래된 말초혈 임파구로부터 선택되는 방법.
- 제2항에 있어서, 상기 말초혈 단핵세포 또는 말초혈 임파구가 사람의 세포인 방법.
- 제3항에 있어서, 말초혈 단핵세포 또는 그로부터 유래된 말초혈 임파구를 약 500pM 내지 50,000pM농도의 재조합 인터루킨-2의 존재하에 2 내지 7일간 배양시키는 방법.
- 제4항에 있어서, 말초혈 단핵세포 또는 말초혈 임파구를 약 35℃ 내지 약 39℃ 온도에서 배양시키는 방법.
- 제5항에 있어서, 말초혈 단핵세포 또는 말초혈 임파구를 약 3 내지 약 10용적% CO2대기하에서 배양시키는 방법.
- 제6항에 있어서, 말초혈 임파구가 사용되는 방법.
- 제7항에 있어서, 말초혈 임파구의 농도가 약 1×106세포/ml 내지 약 5×107세포/ml인 방법.
- 제8항에 있어서, 말초혈 임파구를 3 내지 5일 동안 배양시키는 방법.
- 제9항에 있어서, 재조합 인터루킨-2 농도가 약 1000pM 내지 약 2000pM인 방법.
- 제10항에 있어서, 공중합체 필름물질이 에틸렌 및 탄소원자수 4 내지 10인 α-올레핀의, 밀도가 약 0.915 내지 0.925g/cm3인 공중합체로 필수적으로 구성된 방법.
- 제11항에 있어서, 탄소원자수 4 내지 10인 α-올레핀이 1-부텐 또는 1-옥텐이고 상기 공중합체 필름 물질의 두께가 약 0.04mm 내지 약 0.14mm인 방법.
- 제12항에 있어서, 탄소원자수 4 내지 10인 α-올레핀이 1-옥텐인 방법.
- 제13항에 있어서, 용기가 열봉합에 의해 밀폐된 방법.
- 제14항에 있어서, 말초혈 임파구의 농도가 약 5×106내지 약 1.5×107세포/ml인 방법.
- 제15항에 있어서, 말초혈 임파구를 사람의 혈청알부민이 첨가된 RPMI 1640 배지에서 배양시키는 방법.
- 제1항 내지 6항 중 어느 한 항에 있어서, 배양하기 전에, 말초혈 단핵세포 또는 말초혈 임파구를 L-아미노산 저급 알킬 에스테르 또는 그의 염산염과 접촉시키고, 이때 상기 L-아미노산은 패닐알라닌, 글루탐산, 글루타민 및 티로신 및 그의 혼합물로 구성된 그룹으로부터 선택되는 방법.
- 제11항, 13항 또는 16항 중 어느 한 항에 있어서, 배양하기 전에, 말초혈 임파구를 L-아미노산 저급 알킬에스테르 또는 그의 염산염과 접촉시키고, 이때 상기 L-아미노산은 페닐알라닌, 글루탐산, 글루타민 및 티로신 및 그의 혼합물로 구성된 그룹으로부터 선택되어지는 방법.
- 제17항에 있어서, L-아미노산 저급 알킬에스테르가 페닐알라닌 메틸 에스테르인 방법.
- 제18항에 있어서, L-아미노산 저급 알킬에스테르가 페닐알라닌 메틸 에스테르인 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US827387A | 1987-01-29 | 1987-01-29 | |
| US008273 | 1987-01-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR880009123A true KR880009123A (ko) | 1988-09-14 |
| KR900004421B1 KR900004421B1 (ko) | 1990-06-25 |
Family
ID=21730714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019880000685A KR900004421B1 (ko) | 1987-01-29 | 1988-01-28 | 활성화된 킬러세포의 개선된 제조방법 |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0280054B1 (ko) |
| JP (1) | JPH06104060B2 (ko) |
| KR (1) | KR900004421B1 (ko) |
| AT (1) | ATE82319T1 (ko) |
| AU (1) | AU609768B2 (ko) |
| CA (1) | CA1300058C (ko) |
| DE (1) | DE3875752T2 (ko) |
| DK (1) | DK42188A (ko) |
| ES (1) | ES2052611T3 (ko) |
| IE (1) | IE880224L (ko) |
| IL (1) | IL85238A (ko) |
| NZ (1) | NZ223326A (ko) |
| ZA (1) | ZA88656B (ko) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8826298D0 (en) * | 1988-11-10 | 1988-12-14 | Williams N S | Production of lak cells compositions containing them & their use in cancer therapy |
| WO1990010059A1 (en) * | 1989-02-21 | 1990-09-07 | Terumo Corporation | A process for the generation of proliferating cd4 lymphocytes |
| CA2015294A1 (en) * | 1989-04-28 | 1990-10-28 | John J. Rinehart | Generation and expansion of lak cells |
| US6190913B1 (en) * | 1997-08-12 | 2001-02-20 | Vijay Singh | Method for culturing cells using wave-induced agitation |
| US7745209B2 (en) | 2005-07-26 | 2010-06-29 | Corning Incorporated | Multilayered cell culture apparatus |
| CN101611133A (zh) | 2006-12-07 | 2009-12-23 | 威尔森沃尔夫制造公司 | 高效装置及培养细胞的方法 |
| DE102011106914B4 (de) | 2011-07-08 | 2015-08-27 | Zellwerk Gmbh | Mäander- Bioreaktor und Verfahren zu dynamischen Expansion, Differenzierung und Ernte von hämatopoetischen Zellen |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4140162A (en) * | 1977-07-28 | 1979-02-20 | Baxter Travenol Lab | Clear, autoclavable plastic formulation free of liquid plasticizers |
| US4280497A (en) * | 1979-10-09 | 1981-07-28 | Cutter Laboratories, Inc. | Container for platelet storage |
| GB2065067B (en) * | 1979-10-15 | 1983-06-22 | Toppan Printing Co Ltd | Laminated bags |
| JPS5829465A (ja) * | 1981-08-05 | 1983-02-21 | イ−・アイ・デユ・ポン・ドウ・ヌム−ル・アンド・カンパニ− | 血小板貯蔵容器 |
| US4496361A (en) * | 1981-08-05 | 1985-01-29 | E. I. Du Pont De Nemours And Company | Platelet storage container |
| US4588401A (en) * | 1982-06-29 | 1986-05-13 | E. I. Du Pont De Nemours And Company | Platelet storage container |
| JPS60160881A (ja) * | 1984-01-03 | 1985-08-22 | イ−・アイ・デユポン・ド・ネモア−ス・アンド・コンパニ− | 細胞培養室のための薄いフイルム |
-
1988
- 1988-01-26 CA CA000557378A patent/CA1300058C/en not_active Expired - Lifetime
- 1988-01-27 AT AT88101138T patent/ATE82319T1/de not_active IP Right Cessation
- 1988-01-27 ES ES88101138T patent/ES2052611T3/es not_active Expired - Lifetime
- 1988-01-27 EP EP88101138A patent/EP0280054B1/en not_active Expired - Lifetime
- 1988-01-27 DE DE8888101138T patent/DE3875752T2/de not_active Expired - Fee Related
- 1988-01-27 AU AU10761/88A patent/AU609768B2/en not_active Ceased
- 1988-01-27 NZ NZ223326A patent/NZ223326A/xx unknown
- 1988-01-28 KR KR1019880000685A patent/KR900004421B1/ko not_active IP Right Cessation
- 1988-01-28 IL IL85238A patent/IL85238A/xx unknown
- 1988-01-28 ZA ZA88656A patent/ZA88656B/xx unknown
- 1988-01-28 IE IE880224A patent/IE880224L/xx unknown
- 1988-01-28 DK DK042188A patent/DK42188A/da not_active Application Discontinuation
- 1988-01-29 JP JP63017516A patent/JPH06104060B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0280054B1 (en) | 1992-11-11 |
| AU1076188A (en) | 1988-08-04 |
| ATE82319T1 (de) | 1992-11-15 |
| DK42188A (da) | 1988-07-30 |
| DK42188D0 (da) | 1988-01-28 |
| IE880224L (en) | 1988-07-29 |
| DE3875752T2 (de) | 1993-04-22 |
| AU609768B2 (en) | 1991-05-09 |
| EP0280054A1 (en) | 1988-08-31 |
| JPS63202378A (ja) | 1988-08-22 |
| ZA88656B (en) | 1989-09-27 |
| KR900004421B1 (ko) | 1990-06-25 |
| NZ223326A (en) | 1989-10-27 |
| IL85238A0 (en) | 1988-07-31 |
| DE3875752D1 (de) | 1992-12-17 |
| ES2052611T3 (es) | 1994-07-16 |
| CA1300058C (en) | 1992-05-05 |
| JPH06104060B2 (ja) | 1994-12-21 |
| IL85238A (en) | 1992-03-29 |
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