KR860006543A - 인터페론-γ의 제조방법 - Google Patents

인터페론-γ의 제조방법 Download PDF

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KR860006543A
KR860006543A KR1019860000675A KR860000675A KR860006543A KR 860006543 A KR860006543 A KR 860006543A KR 1019860000675 A KR1019860000675 A KR 1019860000675A KR 860000675 A KR860000675 A KR 860000675A KR 860006543 A KR860006543 A KR 860006543A
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strain
gram
belonging
expression system
positive
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KR1019860000675A
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English (en)
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유따까(외 3) 이시다
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마쓰시다 렌조
가부시끼가이샤 미도리쥬지
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Publication of KR860006543A publication Critical patent/KR860006543A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

인터페론-γ의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 pGC 303의 제한효소지도이며, 제3도는 이 콜라이 발현 벡터의 작성 공정을 나타낸다. 숫자는 본문중의 긍정번호를 나타낸다.
제5도는 pYI 7-1과 IFN-γ유전자와의 연결에 의한 pIr amy5의 작성공정을 나타내며,

Claims (4)

  1. 발현계 유전자로서 그람양성 균주로부터 유도된 α-아미라제 프로모터 부분을 함유하는 발현계 및 숙주로서 그람 음성균주를 사용하여 인터페론-γ를 제조하는 방법.
  2. 제1항에서, 그람양성 균주가 바실루스 속에 속하는 균주인 방법.
  3. 제2항에서 바실루스 속에 속하는 균주가 바실루스 나토종에 속하는 균주인 방법.
  4. 제1-4항에서 그람 음성 균주가 이 콜라이의 균주인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860000675A 1985-02-02 1986-01-31 인터페론-γ의 제조방법 KR860006543A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP18991 1985-02-02
JP60018991A JPS61177996A (ja) 1985-02-02 1985-02-02 インタ−フエロン−γの製造法

Publications (1)

Publication Number Publication Date
KR860006543A true KR860006543A (ko) 1986-09-13

Family

ID=11987034

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019860000675A KR860006543A (ko) 1985-02-02 1986-01-31 인터페론-γ의 제조방법

Country Status (4)

Country Link
EP (1) EP0190686A3 (ko)
JP (1) JPS61177996A (ko)
KR (1) KR860006543A (ko)
ES (1) ES8800361A1 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61135599A (ja) * 1984-12-04 1986-06-23 Green Cross Corp:The 異種蛋白質の製造法
JP2791418B2 (ja) 1987-12-02 1998-08-27 株式会社ミドリ十字 異種蛋白質の製造方法、組換えｄｎａ、形質転換体
BG52073B2 (en) * 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
JPS6027388A (ja) * 1983-07-22 1985-02-12 Green Cross Corp:The プラスミツド
JPS61135599A (ja) * 1984-12-04 1986-06-23 Green Cross Corp:The 異種蛋白質の製造法

Also Published As

Publication number Publication date
ES8800361A1 (es) 1987-10-16
ES551424A0 (es) 1987-10-16
JPS61177996A (ja) 1986-08-09
EP0190686A2 (en) 1986-08-13
EP0190686A3 (en) 1988-01-07

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