KR860003339A - 고정된 효소를 함유하는 다공성 물질의 제조방법 - Google Patents

고정된 효소를 함유하는 다공성 물질의 제조방법 Download PDF

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KR860003339A
KR860003339A KR1019850007922A KR850007922A KR860003339A KR 860003339 A KR860003339 A KR 860003339A KR 1019850007922 A KR1019850007922 A KR 1019850007922A KR 850007922 A KR850007922 A KR 850007922A KR 860003339 A KR860003339 A KR 860003339A
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enzyme
inorganic
cells
mixture
suspension
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KR1019850007922A
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KR920009499B1 (ko
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후미히로 이시무라 (외 3)
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다까다 데쯔오 (외 1)
도요 요조 가부시끼 가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

내용 없음

Description

고정된 효소를 함유하는 다공성 물질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (17)

  1. 비누화도가 95몰% 이상이고, 평균 중합도가 1,000 이상인 폴리비닐 알콜 수용액, 효소함유 물질 및 활성탄 분말을 혼합하고, 혼합물을 목적하는 형태의 용기에 붓고, 이 혼합물을 탄수율이 50% 이상 되도록 탈수시켜 겔화 및 성형시키고 수득된 성형물을 물과 접촉시켜 침지 물질을 수득하고, 침지 물질을 건조시킴을 특징으로 하여,고정된 효소를 함유하는 단단한 다공성 물질을 제조하는 방법.
  2. 제1항에 있어서, 폴리비닐 알콜 수용액의 농도가 5 내지 30W/W% 범위인 방법.
  3. 제1항에 있어서, 효소 함유 물질이 효소제제이거나 효소를 생성하는 미생물 세포인 방법.
  4. 제3항에 있어서, 효소 제제가 (a)효소, (b)효소 및 무기 또는 유기 담체의 혼합물, 또는 (c)효소 및 무기 또는 유기 담체의 복합 물질인 방법.
  5. 제3항에 있어서, 효소 제제가 상기(b) 또는 (c)의 현탁액인 방법.
  6. 제3항에 있어서, 효소 제제가 (d)효소액, (e)효소액 및 무기 또는 유기 담체의 혼합물, 또는 (f)효소액 및 무기 또는 유기 담체의 복합물인 방법.
  7. 제3항에 있어서, 효소 제제가 상기 (e) 또는 (f)의 현탁액인 방법.
  8. 제3항에 있어서, 효소를 생성하는 미생물 세포가 생세포, 건조세포체, 파괴세포체, (g) 이러한 세포체 및 무기 또는 유기 담체의 혼합물, 또는 (h)이러한 세포 및 무기 또는 유기 담체의 복합물질인 방법.
  9. 제3항에 있어서, 효소 생성 세포가 생세포, 건조세포 또는 파괴 세포의 현탁액이거나 상기 (g) 또는 (h)의 현탁액인 방법.
  10. 제1항에 있어서,활성탄을 폴리비닐알콜 중량에 대해 8 내지 40중량% 범위로 사용하는 방법.
  11. 제1항에 있어서, 용기가 과립·봉·판 형태인 방법.
  12. 제1항에 있어서, 자연건조 또는 공기 건조시켜 탈수시키는 방법.
  13. 제1항에 있어서, 물에 침지시키거나 물을 분무하여 물과 접촉시키는 방법.
  14. 제13항에 있어서, 효소 활성을 감소시키기 않는 온도 범위내의 조건하에서 물과 접촉시키는 방법.
  15. 제1항에 있어서, 침지 물질이 봉 또는 판 형태인 경우 침지물질을 미세과립화시킬 수 있는 방법.
  16. 제1항에 있어서, 자연 건조 또는 공기 건조로써 건조시키는 방법.
  17. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019850007922A 1984-10-26 1985-10-26 Pva-겔에 의해 고정된 효소를 함유하는 다공성 물질의 제조방법 KR920009499B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP59223829A JPS61104787A (ja) 1984-10-26 1984-10-26 Ｐｖａゲルによる多孔性固定化酵素含有物の製法
JP223829/84 1984-10-26

Publications (2)

Publication Number Publication Date
KR860003339A true KR860003339A (ko) 1986-05-23
KR920009499B1 KR920009499B1 (ko) 1992-10-17

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KR1019850007922A KR920009499B1 (ko) 1984-10-26 1985-10-26 Pva-겔에 의해 고정된 효소를 함유하는 다공성 물질의 제조방법

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US (1) US4727030A (ko)
JP (1) JPS61104787A (ko)
KR (1) KR920009499B1 (ko)

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JP2639112B2 (ja) * 1989-06-30 1997-08-06 キヤノン株式会社 マクロ機構付テレビレンズ
DE4027218C1 (ko) * 1990-08-24 1991-09-19 Vorlop, Klaus-Dieter, Dr., 3300 Braunschweig, De
US5288491A (en) * 1992-09-24 1994-02-22 Herbert Moniz Noni (Morinda Citrifolia) as a pharmaceutical product
WO1998015620A1 (en) * 1996-10-10 1998-04-16 Biotechnology Research And Development Corporation Polymer-protein composites and methods for their preparation and use
US6291582B1 (en) 1996-10-10 2001-09-18 Biotechnology Research & Development Corp. Polymer-protein composites and methods for their preparation and use
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WO2003106655A2 (en) * 2002-06-18 2003-12-24 The University Of Akron Fibrous protein-immobilization systems
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JP4432050B2 (ja) * 2005-06-06 2010-03-17 株式会社日立プラントテクノロジー 包括固定化担体の製造方法及び装置
US8222015B2 (en) * 2007-05-11 2012-07-17 Toyota Motor Engineering & Manufacturing North America, Inc. Heat resistant bioactive composition
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AU2018444217B2 (en) * 2018-10-03 2022-04-21 Nippon Steel Engineering Co., Ltd. Production method for briquette
CN111333200B (zh) * 2020-03-18 2022-07-05 运城学院 一种包埋固定化微生物颗粒、制备方法及污水处理方法

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US4727030A (en) 1988-02-23
KR920009499B1 (ko) 1992-10-17
JPH0234B2 (ko) 1990-01-05
JPS61104787A (ja) 1986-05-23

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