JPS57141291A - Immobilization of live microbial cell - Google Patents

Immobilization of live microbial cell

Info

Publication number
JPS57141291A
JPS57141291A JP2688781A JP2688781A JPS57141291A JP S57141291 A JPS57141291 A JP S57141291A JP 2688781 A JP2688781 A JP 2688781A JP 2688781 A JP2688781 A JP 2688781A JP S57141291 A JPS57141291 A JP S57141291A
Authority
JP
Japan
Prior art keywords
microbial cells
polyvinyl alcohol
clay mineral
suspension
article
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2688781A
Other languages
Japanese (ja)
Inventor
Masao Nanbu
Sadao Sakayanagi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eneos Corp
Original Assignee
Nippon Oil Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Oil Corp filed Critical Nippon Oil Corp
Priority to JP2688781A priority Critical patent/JPS57141291A/en
Priority to CA000396893A priority patent/CA1180670A/en
Priority to EP82300914A priority patent/EP0060052A1/en
Priority to AU80764/82A priority patent/AU8076482A/en
Priority to BR8201032A priority patent/BR8201032A/en
Publication of JPS57141291A publication Critical patent/JPS57141291A/en
Pending legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PURPOSE: To immobilize microbial cells without using reaction reagents, etc., by preparing an aqueous suspension of a clay mineral having lamellar structure and live microbial cells dispersed in an aqueous solution of a polyvinyl alcohol having specific saponification degree and viscosity-average polymerization degree, casting the suspension in a mold, followed by freezing, forming, and drying to a definite water content.
CONSTITUTION: A clay mineral having lamellar structure consisting of a ternary composite layer as the basic unit, e.g. bentonits, is added to an aqueous solution of a polyvinyl alcohol having a saponification degree of ≥95mol% and a viscosity-average polymerization degree of ≥1,500. The weight ratio of the clay mineral to the polyvinyl alcohol is ≤5. After adjusting the pH of the suspension to the optimum level for the microbial cells to be used, live microbial cells are added thereto, and the mixture is poured into a vessel having desired shape or into a forming mold, and frozen and formed at ≤-6°C. The formed article is dried to a dehydration ratio of ≥5wt% in vacuum without melting the article. The dried article can be reconstituted to immobilized microbial cells by immersing in water at need.
COPYRIGHT: (C)1982,JPO&Japio
JP2688781A 1981-02-27 1981-02-27 Immobilization of live microbial cell Pending JPS57141291A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2688781A JPS57141291A (en) 1981-02-27 1981-02-27 Immobilization of live microbial cell
CA000396893A CA1180670A (en) 1981-02-27 1982-02-23 Method of immobilizing live microorganisms
EP82300914A EP0060052A1 (en) 1981-02-27 1982-02-23 Method of immobilizing live microorganisms
AU80764/82A AU8076482A (en) 1981-02-27 1982-02-24 Immobilising live microorganisms
BR8201032A BR8201032A (en) 1981-02-27 1982-03-01 PROCESS FOR IMMOBILIZATION OF LIVE MICROORGANISMS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2688781A JPS57141291A (en) 1981-02-27 1981-02-27 Immobilization of live microbial cell

Publications (1)

Publication Number Publication Date
JPS57141291A true JPS57141291A (en) 1982-09-01

Family

ID=12205768

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2688781A Pending JPS57141291A (en) 1981-02-27 1981-02-27 Immobilization of live microbial cell

Country Status (1)

Country Link
JP (1) JPS57141291A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61158786A (en) * 1984-12-28 1986-07-18 Susumu Hashimoto Preparation of immobilized microorganism embedded in carrier and group of immobilized microorganism embedded in carrier

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61158786A (en) * 1984-12-28 1986-07-18 Susumu Hashimoto Preparation of immobilized microorganism embedded in carrier and group of immobilized microorganism embedded in carrier
JPS6329997B2 (en) * 1984-12-28 1988-06-16 Susumu Hashimoto

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